The effect of immobilization, drying and storage on the activity of proteinases immobilized on modified cellulose and chitosan

Dosadina, Elina E.; Savelyeva, Elizaveta Evgenyevna; Белов, А.А.

doi:10.1016/j.procbio.2017.10.002

Cited by 13 publications

(3 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…БМ на основе коллагена также являются биодеградируемыми; химические вещества, образующиеся при разрушении коллагеновых белков не токсичны. Имеются данные о том, что БМ на основе коллагена стимулируют восстановительные процессы в тканях [29,36,61].…”

Section: биопластические материалы и репаративные гистогенезыunclassified

“…[2] в эксперименте на крысах показан при лечении гнойно-некротических ран продемонстрирован позитивный эффект применения раневого покрытия в виде высокопористой губки, созданной на основе хитозанколлагенового комплекса с наночастицами серебра. Схожие эффекты использования БМ на основе хитина и хитозана при лечении ран имеются и у других исследователей [25,36,47,57]. Этими авторами показано, что использование хитина либо хитозана в комплексе с коллагеном ускоряет заживление гнойных ран за счет активизации роста и цитодифференцировки клеток соединительной ткани (прежде всего, клеток фибробластического дифферона) и эпителия, при этом коллагеновый каркас служит матрицей для роста соединительной ткани, что затем способствует и ускорению роста эпителия по новой соединительной ткани.…”

Section: биопластические материалы и репаративные гистогенезыunclassified

See 1 more Smart Citation

Features of Reparative Histogenesis in Bioplastic Material Application

Shevlyuk

Gatiatullin

Стадников

2020

Žurnal anatomii i gistopatologii

View full text Add to dashboard Cite

In modern medicine, various biocompatible materials (based on biodegradable natural biopolymers – collagen, hyaluronic acid, chitin, chitosan, etc.) are widely used, primarily for the purposes of reconstructive and plastic surgery. The development of these materials and their introduction into clinical practice is an extremely urgent task of regenerative biology and medicine. One of the most important properties of bioplastic materials is their ability to undergo biodegradation and gradually be replaced by the recipient's proper tissues. In this case, the intermediate and final metabolic products of these materials should be included in the natural biochemical cycles of the body without their systemic and local accumulation, and degradation products should lack the toxicity effect. Bioplastic materials can also serve as carriers of biologically active substances, for example, growth factors and morphogenetic proteins, antibacterial substances, as well as pharmacological agents that affect the rate of regeneration. The designed three-dimensional porous structure of new materials, morphologically similar to the structure of body tissues, allows them to ensure the migration of fibroblastic cells, the growth of blood vessels in the area occupied by this material, that is, they can serve as a skeleton (matrix), a basis for histio- and organotypic regenerates developing in various organs. Many bioplastic materials have the ability to enhance angiogenesis, and are also able to activate proliferation and cytodifferentiation of epithelial cells and fibroblast differentiation cells of the connective tissue, which leads to the formation of young connective tissue in the transplant zone and epithelization of organ damage. Thus, biocompatible and biodegradable polymers are able to stimulate reparative histogenesis, providing optimal conditions for the formation of histio- and organotypic regenerates of various tissues and organs.

show abstract

Section: биопластические материалы и репаративные гистогенезыunclassified

Features of Reparative Histogenesis in Bioplastic Material Application

Shevlyuk

Gatiatullin

Стадников

2020

Žurnal anatomii i gistopatologii

View full text Add to dashboard Cite

show abstract

“…Enzyme immobilization on a solid support provides good distribution of the catalysts with less aggregation. On the other hand, covalent binding between the support and the enzyme results in increased enzymatic stability, which in turn leads to enhanced enzymatic activity [1,20,21].…”

Section: Introductionmentioning

confidence: 99%

Recent Advances of Cellulase Immobilization onto Magnetic Nanoparticles: An Update Review

et al. 2019

View full text Add to dashboard Cite

Cellulosic enzymes, including cellulase, play an important role in biotechnological processes in the fields of food, cosmetics, detergents, pulp, paper, and related industries. Low thermal and storage stability of cellulase, presence of impurities, enzyme leakage, and reusability pose great challenges in all these processes. These challenges can be overcome via enzyme immobilization methods. In recent years, cellulase immobilization onto nanomaterials became the focus of research attention owing to the surface features of these materials. However, the application of these nanomaterials is limited due to the efficacy of their recovery process. The application of magnetic nanoparticles (MNPs) was suggested as a solution to this problem since they can be easily removed from the reaction mixture by applying an external magnet. Recently, MNPs were extensively employed for enzyme immobilization owing to their low toxicity and various practical advantages. In the present review, recent advances in cellulase immobilization onto functionalized MNPs is summarized. Finally, we discuss enhanced enzyme reusability, activity, and stability, as well as improved enzyme recovery. Enzyme immobilization techniques offer promising potential for industrial applications.

show abstract

Kinetic, stability, and activity of the nanoparticles-immobilized enzymes

Slováková¹

2023

Antimicrobial Dressings

View full text Add to dashboard Cite

The effect of immobilization, drying and storage on the activity of proteinases immobilized on modified cellulose and chitosan

Cited by 13 publications

References 20 publications

Features of Reparative Histogenesis in Bioplastic Material Application

Features of Reparative Histogenesis in Bioplastic Material Application

Recent Advances of Cellulase Immobilization onto Magnetic Nanoparticles: An Update Review

Kinetic, stability, and activity of the nanoparticles-immobilized enzymes

Contact Info

Product

Resources

About