The Effect of Inflammation on the Hexuronate-Containing Polysaccharides of Human Plasma1

Kerby, Grace P.

doi:10.1172/jci103691

Cited by 24 publications

(6 citation statements)

References 5 publications

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“…The factor (or factors) which restored the dialyzed plasma to its nondialyzed state in regard to S1 precipitability was dialyzable, resisted heating to 100°C for 15 minutes, but was destroyed by ashing. Addition of certain known dialyzable plasma components and other substances to the buffered balanced tions were found to be increased in the inflammatory groups II and III, as expected from previous studies (1). The euglobulin data are summarized in Figure 3, which plots protein content of the fraction against its cholesterol content.…”

Section: Methodssupporting

confidence: 77%

“…In surveying patients, oxalated blood for plasma was obtained from three groups of individuals, as previously defined in detail (1). Briefly, group I showed no evidence of inflammatory disease and had negative C-reactive protein tests; group II had significant inflammatory disease unrelated to rheumatoid or other primary connective tissue disease; and group III had clinically unequivocal active rheumatoid arthritis, in no instance being treated with hormones.…”

Section: Methodsmentioning

confidence: 99%

“…Results were expressed as milligrams per 100 ml of original plasma. A standard curve of 10, 25, 50, 100 jug of puri- 1 Ten ml of stock solution (7.5 Table I illustrates the striking increase in protein available for precipitation in the S1 fraction after dialysis of the original plasma. This was a consistent finding with all plasmas.…”

Section: Methodsmentioning

confidence: 99%

“…Acid mucopolysaccharides of human plasma have been observed ( 1 ) to vary as do acute phase reactants, i.e., to increase nonspecifically in any inflammatory state. The euglobulin fraction itself with which the acid mucopolysaccharides were associated increased also in such circumstances.…”

mentioning

confidence: 99%

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A Lipid-Protein Fraction in Rheumatoid Plasma Precipitable With Chondroitin Sulfate After Euglobulin Removal*

Kerby¹,

Taylor²,

Langley³

1961

J. Clin. Invest.

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Acid mucopolysaccharides of human plasma have been observed ( 1 ) to vary as do acute phase reactants, i.e., to increase nonspecifically in any inflammatory state. The euglobulin fraction itself with which the acid mucopolysaccharides were associated increased also in such circumstances. On later comparing levels of acid mucopolysaccharides in samples of serum and plasma drawn simultaneously, it was noted that the acid mucopolysaccharides measurable in the euglobulin fraction were derived at least in part from the formed elements of blood during blood clotting (2). It seemed possible, therefore, that the amount of plasma protein precipitable as euglobulins by dilution at pH 5.8 might increase in inflammation as a result of the release of acid mucopolysaccharides into the blood, causing alteration of isoelectric point of plasma proteins already present. Precedent for such a possibility has been well established by the earlier observations of other investigators on the interactions of fibrinogen and ,8-lipoproteins and polyanions (3, 4). The possibility as it relates to rheumatoid plasma was mentioned in effect also bv Badin, Schubert and Vouras (5).To explore this possibility, studies were carried out on the interaction of chondroitin sulfate with the proteins remaining in plasma after isoelectric precipitation of spontaneously occurring euglobulins by dilution with mineral acid to pH 5.8. The additional protein available for precipitation under identical conditions of dilution and pH on addition of chondroitin sulfate (the fraction being called Sj) was strikingly increased by prior dialysis of the plasma against balanced bufferred salt solution. This increase was prevented if a heat-resistant, organic, dialyzable factor present in a pressure dialy-sate of plasma was restored

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Section: Methodssupporting

confidence: 77%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

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A Lipid-Protein Fraction in Rheumatoid Plasma Precipitable With Chondroitin Sulfate After Euglobulin Removal*

Kerby¹,

Taylor²,

Langley³

1961

J. Clin. Invest.

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“…As described in this paper, all urinary mucopolysaccharides are also increased when exophthalmos is present. In this condition mucopolysaccharides containing sulfoamino groups (heparin and/or heparitin sulfate) may be increased to a proportionately greater amount than other AMPS.2 Greater than normal amounts of plasma mucopolysaccharides have been found in patients with rheumatoid arthritis, and in those with inflammatory conditions secondary to bacterial infection or traumatic injuries (27,28). The increase of serum AMPS in exophthalmos described in this communication is greater than the increase thus far described in other diseases.…”

Section: Resultsmentioning

confidence: 63%