The Effect of Muscle Actions on the Level of Connective Tissue Damage

Nogueira, Antonio de Carvalho; Vale, Rodrigo Gomes de Souza; Gomes, André Luiz Marques; Dantas, Estélio Henrique Martín

doi:10.1080/15438627.2011.608046

Cited by 18 publications

(13 citation statements)

References 20 publications

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“…Previous studies have demonstrated that 6-hydroxynicotinate and 2-heptyl-3-hydroxy 4-quinolone are essentially produced from bacterial metabolism2324 and possibly originated from tissue degradation during sample transportation and storage. Hydroxyproline, a major constituent of collagen, is also used as an indicator of tissue damage or degradation25.…”

Section: Resultsmentioning

confidence: 99%

In vivo microsampling to capture the elusive exposome

Bessonneau

Ings

McMaster

et al. 2017

Sci Rep

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Loss and/or degradation of small molecules during sampling, sample transportation and storage can adversely impact biological interpretation of metabolomics data. In this study, we performed in vivo sampling using solid-phase microextraction (SPME) in combination with non-targeted liquid chromatography and high-resolution tandem mass spectrometry (LC-MS/MS) to capture the fish tissue exposome using molecular networking analysis, and the results were contrasted with molecular differences obtained with ex vivo SPME sampling. Based on 494 MS/MS spectra comparisons, we demonstrated that in vivo SPME sampling provided better extraction and stabilization of highly reactive molecules, such as 1-oleoyl-sn-glycero-3-phosphocholine and 1-palmitoleoyl-glycero-3-phosphocholine, from fish tissue samples. This sampling approach, that minimizes sample handling and preparation, offers the opportunity to perform longitudinal monitoring of the exposome in biological systems and improve the reliability of exposure-measurement in exposome-wide association studies.

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Section: Resultsmentioning

confidence: 99%

In vivo microsampling to capture the elusive exposome

Bessonneau

Ings

McMaster

et al. 2017

Sci Rep

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“…The hydroxyproline is formed via post-translational modification of pro-collagen where residues of proline (and lysine) are hydroxylated to form the final collagen product. Hydroxyproline is primarily found in collagen, and the appearance of it in urine can be used as a measure of collagen turnover [45, 46]. It was thus proposed that females from cluster 1 were susceptible to experiencing higher levels of fatigue and pain if they had higher urinary levels of the collagen components hydroxyproline, hydroxylysine and proline.…”

Section: Discussionmentioning

confidence: 99%

“…The formulation of the amino acid supplement was such that it would have provided a direct source of amino acids capable of reducing the requirement for non-myofibrillar catabolism and therefore it had the potential to relieve fatigue. The higher levels of urinary histidine (cluster 1) and glycine (cluster 2) could have contributed to limitations in haemoglobin production and collagen turnover respectively and provision of both histidine and glycine in the supplement may have led to enhancements in the synthesis of these proteins [33, 34, 38, 45] and a reduction in associated symptoms such as fatigue and pain.…”

Section: Discussionmentioning

confidence: 99%

Diverse characteristics of the urinary excretion of amino acids in humans and the use of amino acid supplementation to reduce fatigue and sub-health in adults

Dunstan

Sparkes

Macdonald

et al. 2017

Nutr J

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BackgroundThe excretion of amino acids in urine represents an important avenue for the loss of key nutrients. Some amino acids such as glycine and histidine are lost in higher abundance than others. These two amino acids perform important physiological functions and are required for the synthesis of key proteins such as haemoglobin and collagen.MethodsStage 1 of this study involved healthy subjects (n = 151) who provided first of the morning urine samples and completed symptom questionnaires. Urine was analysed for amino acid composition by gas chromatography. Stage 2 involved a subset of the initial cohort (n = 37) who completed a 30 day trial of an amino acid supplement and subsequent symptom profile evaluation.ResultsAnalyses of urinary amino acid profiles revealed that three groups could be objectively defined from the 151 participants using k-means clustering. The amino acid profiles were significantly different between each of the clusters (Wilks’ Lambda = 0.13, p < 0.0001). Cluster 1 had the highest loss of amino acids with histidine being the most abundant component. Cluster 2 had glycine present as the most abundant urinary amino acid and cluster 3 had equivalent abundances of glycine and histidine. Strong associations were observed between urinary proline concentrations and fatigue/pain scores (r = .56 to .83) for females in cluster 1, with several other differential sets of associations observed for the other clusters.ConclusionsDifferent phenotypic subsets exist in the population based on amino acid excretion characteristics found in urine. Provision of the supplement resulted in significant improvements in reported fatigue and sleep for 81% of the trial cohort with all females reporting improvements in fatigue.Trial registrationThe study was registered on the 18th April 2011 with the Australian New Zealand Clinical Trials Registry (ACTRN12611000403932).

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“…4-hydroxyproline is a major component of the protein collagen and declining levels of urinary 4-hydroxyproline are indicative of muscle loss [31]. L-glutamine occupies a major proportion of free intracellular amino acids in the total pool of muscle [32], and degradation level of muscle protein is associated with content of free amino acids like L-glutamine.…”

Section: Metabolic Responses To 520 Days' Isolationmentioning

confidence: 99%

Urinary Metabolic Profiling of ‘MARS-500 Project’ with Nuclear Magnetic Resonance Spectroscopy

Chen¹,

Yu²,

Dong³

2015

Metabolomics

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# These authors contributed equally to this work. metabolic profiles through analyzing complex biofluids (e.g., urine and plasm) can provide important diagnostic and prognostic information [8]. Among the biofluids most commonly analyzed in metabolomic studies, urine appears to be particularly useful, because it is abundant, readily available, easily stored and can be collected by simple, noninvasive techniques [9]. Although, persistent alterations induced by dietary or chronic interventions may also be detected from plasma, but it just provides a description of the metabolic system at the time of sampling, and yet some biochemical or pathological changes may not be specific to external stimuli or genetic modification. By contrast, information from urine is time-averaged because of its collection and storage in the bladder [8]. Therefore, urine was selected to conduct metabolic mapping in many publications [10][11][12]. Among analytical techniques used in metabonomics, nuclear magnetic resonance (NMR) [13] and mass spectroscopy (MS) [14] possessed the dominant status. As known, NMR has the advantage of being rapid, high reproducibility, nondestructive to samples, applicable to intact biomaterials and rich in chemical structural information. In addition, unlike GC-MS and certain liquid chromatography-mass spectrometry (LC-MS) methods,

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The Effect of Muscle Actions on the Level of Connective Tissue Damage

Cited by 18 publications

References 20 publications

In vivo microsampling to capture the elusive exposome

In vivo microsampling to capture the elusive exposome

Diverse characteristics of the urinary excretion of amino acids in humans and the use of amino acid supplementation to reduce fatigue and sub-health in adults

Urinary Metabolic Profiling of ‘MARS-500 Project’ with Nuclear Magnetic Resonance Spectroscopy

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