The current study aims to assess the conditions that are relevant for an appropriate method of determination of precaecal P digestibility in broilers by reviewing literature and performing several experiments. The focus of the literature review is on phytate degradation. It's extent depends on various factors, which are assessed in this review. Moreover, four experiments were conducted to determine the effects of phytate level of the basal diet, as well as the level and source of supplemented P on phytate degradation and precaecal P digestibility, as determined according to the WPSA protocol. Summary WPSA has published (2013) a standard protocol for determination of availability of phosphorus (P) in feedstuffs for broilers. It is based on precaecal P digestibility (pcdP). The current study aims to assess the conditions that are relevant for an appropriate method of determination of precaecal P digestibility in broilers by reviewing literature and performing several experiments. The focus of the literature review is on phytate degradation. Although it was assumed that monogastrics are lacking endogenous phytase production, it's known from literature that broilers are able to degrade phytate. The extent of phytate degradation depends on various factors, which are assessed in this review. In the current study, four experiments were conducted to address four different research questions.1. Effect of non-phytate P supplementation to a phytate-low basal diet on phytate degradation and precaecal P digestibility.2. Effect of non-phytate P supplementation to a phytate-rich basal diet on phytate degradation and precaecal P digestibility.3. Effect of phytate P supplementation to a phytate-rich basal diet on phytate degradation, precaecal and faecal P digestibility.4. Effect of dietary structure to a phytate-rich basal diet on phytate degradation and precaecal P digestibility.Experimental diets were fed as pellets to Ross 308 male broilers housed in 6 replicate cages, 10-12 birds per cage from 14 to 24 or 20 to 28 d of age. SBM was the only phytate source and MCP was used as non-phytate P source. Dietary Ca/P ratio was kept at 1.35 and TiO2 was used as inert marker.Feed and water were freely available.Based on findings of the present experiment, the following conclusions can be drawn:• Literature shows that the dietary Ca, P, and Vitamin D content, the Ca:aP ratio and dietary structure affects phytate degradation in broilers.• In phytate-low broiler diets, pcdP-% was constant over a large aP-range.• Phytate degradation showed a large variation among studies. Phytate degradation linearly decreased with increasing dietary non-phytate phosphorus content.• The pcdP-% of SBM determined according to the WPSA protocol does not differ from the faecal P digestibility according to the CVB protocol.• Dietary structure did not affect phytate degradation and pdcP-% of the diet, but increased precaecal Ca digestibility.
RecommendationsIt can be recommended to use phytate-low diets for determining pcdP-% of feed phosphates. Under this co...