The Effect of Recovery Medium on the Isolation of Staphylococcus aureus after Heat Treatment and after the Storage of Frozen or Dried Cells

Baird‐Parker, A. C.; Davenport, E.J.

doi:10.1111/j.1365-2672.1965.tb02169.x

Cited by 112 publications

(48 citation statements)

References 37 publications

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“…It has been suggested that fish are often contaminated with S. aureus in this state. Sodium pyruvate has been reported to recover VBNC cells with damaged metabolic pathways [1]. Introducing an enrichment culture method remarkably increased the S. aureus detection rate in agar (Table 2), which is consistent with previous reports [32,33,38].…”

Section: Discussionsupporting

confidence: 82%

Isolation of Staphylococcus aureus from Raw Fish in Relation to Culture Methods

Saito

Yoshida

Kawano

et al. 2011

The Journal of Veterinary Medical Science

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ABSTRACT. Five hundred and fifty fish samples from various stages in the course of distribution in Hyogo Prefecture (209 retailed in super markets, 173 obtained from fishery cooperatives at a harbor, 91 caught by trawling and 77 caught by rod fishing) were examined for contamination with Staphylococcus aureus (S. aureus). S. aureus was detected in 41 (19.6%) of the retail fish samples and 46 (26.6%) of the samples from the fishery cooperatives. No S. aureus was isolated from the live fish (91 trawled and 77 fished by rod). With regard to the retail fish, the contamination rate of processed fish (26.0%) was significantly higher than that of unprocessed fish (14.2%). For 88 samples, the efficacy of the selective medium was compared using Baird-Parker agar and mannitol salt agar supplemented with egg yolk (MSEY agar) by the direct plate and enrichment culture methods. Using the direct culture method, the S. aureus positive rate with the Baird-Parker agar (30.7%) was significantly higher (P<0.01) than that with the MSEY agar (6.8%). The enrichment culture method remarkably raised the S. aureus detection rate. Seventy-eight (85.7%) of 91 isolates belonged to the human ecovar. Sixty-two (68.1%) of the 91 isolates had some enterotoxin genes, including 44 (48.4%) with the sea gene. These data showed that the fish were contaminated with S. aureus after landing and that Baird-Parker agar had an advantage in detecting S. aureus with a direct plate culture.

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Section: Discussionsupporting

confidence: 82%

Isolation of Staphylococcus aureus from Raw Fish in Relation to Culture Methods

Saito

Yoshida

Kawano

et al. 2011

The Journal of Veterinary Medical Science

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“…Most note increased sensitivity to salt and pH following stress from sub lethal heating, drying or storage. Baird-Parker & Davenport (1965) noted that recovery of stressed cells was best on media which supply catalase or otherwise neutralize peroxides. However, in our experiments, catalase deficiency alone would not appear to be the cause of low recovery as both blood agar and BPA contain agents to neutralize effects of peroxides.…”

Section: Discussionmentioning

confidence: 99%

Studies on the growth and survival ofStaphylococcus aureusin corned beef

Mansfield¹,

Farkas²,

Wieneke

et al. 1983

J. Hyg.

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SUMMARYThe growth of an enterotoxin A producing strain of Staphylococcus aureus in corned beef was investigated. In the inoculated 6 lb. canned product the bacteria spread throughout the meat and attained high numbers. The rate of spread of the organisms was related to the temperature and length of storage of the cans and the numbers of bacteria inoculated. Cans which had been stored for more than four months showed high counts of the bacteria throughout the meat. It was noted that with the long term contaminated product counts of S. aureus on some selective media may give falsely low results.Numbers of S. aureus on meat inoculated by handling after removal from the can were initially extremely variable. More uniform distribution and higher counts were attained only if the meat was exposed for some hours at ambient temperature or above. The significance of the results to the investigation of outbreaks of food poisoning suspected of being associated with canned corned beef is discussed.

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“…Reconstitution of frozen stocks was done by streaking the cultures onto BHI or LB agar followed by incubation at 37°C. Recovery of survivors following stress treatments was carried out on tryptic soy agar supplemented with 0.6% yeast extract (TSA-YE) and 1% sodium pyruvate (4,11). Survival studies for oxidative stress, acid stress, tolerance to ethanol, and osmotic stress were performed in BHI broth augmented according to the methods listed below.…”

Section: Methodsmentioning

confidence: 99%

Increased Thermal and Osmotic Stress Resistance in Listeria monocytogenes 568 Grown in the Presence of Trehalose Due to Inactivation of the Phosphotrehalase-Encoding Gene treA

Ells

Hansen

2011

Appl Environ Microbiol

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The food-borne pathogen Listeria monocytogenes is a problem for food processors and consumers alike, as the organism is resistant to harsh environmental conditions and inimical barriers implemented to prevent the survival and/or growth of harmful bacteria. One mechanism by which listeriae mediate survival is through the accumulation of compatible solutes, such as proline, betaine and carnitine. In other bacteria, including Escherichia coli, the synthesis and accumulation of another compatible solute, trehalose, are known to aid in the survival of stressed cells. The objective of this research was to investigate trehalose metabolism in L. monocytogenes, where the sugar is thought to be transferred across the cytoplasmic membrane via a specific phosphoenolpyruvate phosphotransferase system and phosphorylation to trehalose-6-phosphate (T6P). The latter is subsequently broken down into glucose and glucose-6-phosphate by ␣,␣-(1,1) phosphotrehalase, the putative product of the treA gene. Here we report on an isogenic treA mutant of L. monocytogenes 568 (568:⌬TreA) which, relative to the wild-type strain, displays increased tolerances to multiple stressors, including heat, high osmolarity, and desiccation. This is the first study to examine the putative trehalose operon in L. monocytogenes, and we demonstrate that lmo1254 (treA) in L. monocytogenes 568 indeed encodes a phosphotrehalase required for the hydrolysis of T6P. Disruption of the treA gene results in the accumulation of T6P which is subsequently dephosphorylated to trehalose in the cytosol, thereby contributing to the stress hardiness observed in the treA mutant. This study highlights the importance of compatible solutes for microbial survival in adverse environments.

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The Effect of Recovery Medium on the Isolation of Staphylococcus aureus after Heat Treatment and after the Storage of Frozen or Dried Cells

Cited by 112 publications

References 37 publications

Isolation of <i>Staphylococcus aureus</i> from Raw Fish in Relation to Culture Methods

Isolation of <i>Staphylococcus aureus</i> from Raw Fish in Relation to Culture Methods

Studies on the growth and survival ofStaphylococcus aureusin corned beef

Increased Thermal and Osmotic Stress Resistance in Listeria monocytogenes 568 Grown in the Presence of Trehalose Due to Inactivation of the Phosphotrehalase-Encoding Gene treA

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