The effect of rutin on arginine kinase: Inhibition kinetics and thermodynamics merging with docking simulation

Modulation of Transcriptome and Metabolome of Tobacco by Arabidopsis Transcription Factor, AtMYB12, Leads to Insect Resistance

“…Rutin is an environmentally friendly insecticide. It interacts as a noncompetitive inhibitor with Arg kinase, a key enzyme in the cellular energy metabolism of insects (Wu et al, 2009), which may lead to the insect resistance in AtMYB12-expressing transgenic tobacco lines. Engineering crop plants to develop transgenic seeds with endogenous resistance to insect pests has been one of the major commercial successes of plant biotechnology.…”

Mechanism of inhibition of arginine kinase by flavonoids consistent with thermodynamics of docking simulation

“…Arginine kinase (AK; ATP: l-arginine Nphosphotransferase; EC 2.7.3.3) is widely existed in invertebrates. Inhibitors of AK have been studied widely as potential insecticides because phosphagen kinase in vertebrates is creatine kinase, different from AK in invertebrates [3][4][5]. For instance, after purifying cockroach AK (Periplaneta americana) and investigating the mechanism and models of inhibition of the enzyme, Brown and his colleagues suggested that AK could be a useful chemotherapeutic Abbreviations: AK, arginine kinase; Trp, tryptophan; IC50, the inhibitor concentration leading to 50% activity lost; QU, quencetin; LU, luteolin.…”

Identification and characterization of two arginine kinases from the parasitic insect Ctenocephalides felis

“…Arginine kinase has been proposed as a target for innovative pesticidal compounds Wang et al, 2009;Wu et al, 2009) due to its assumed prominent role in energy metabolism and its absence in vertebrates. However, there are several challenges ahead: the role of AK in insects has not yet been clearly defined and is likely to be distinct in different insects, organs and developmental stages.…”

“…Due to its assumed prominent role in energy metabolism and its specific occurrence in invertebrates, arginine kinase has been proposed as a novel target structure for innovative insecticides to combat insect pest species Wang et al, 2009;Wu et al, 2009), and this enzyme has also attracted our interest as a potential chemotherapeutic target for treatment of ectoparasite infection in veterinary animal species.…”