Identification and characterization of two arginine kinases from the parasitic insect Ctenocephalides felis

Evidence that the amino acid residue Ile121 is involved in arginine kinase activity and structural stability

“…Due to its assumed prominent role in energy metabolism and its specific occurrence in invertebrates, AK has been proposed as a novel target structure for innovative insecticides to combat insect pest species [12][13][14][15]. So, investigation of the amino acids that play important role in keeping AK activity and structural stability will be useful in developing agents to control the insect population and its destructive role in agriculture.…”

Cooperativity in the two-domain arginine kinase from the sea anemone Anthopleura japonicus. II. Evidence from site-directed mutagenesis studies

“…AKs typically function as monomeric enzymes, each with a molecular mass of 40 kDa (singledomain AK). Members of the phosphagen (guanidino) kinase enzyme family, such as AKs and creatine kinases (CKs), play key roles in the ATP buffering systems in animal cells that have high and variable rates of ATP turnover [1][2][3][4][5][6][7][8][9][10]. AK is the most widely distributed among invertebrates and genes encoding AK have been identified in protozoa and bacteria [11][12][13], suggesting its ancient origin [14,15].…”

Characterization of Seafood Proteins Causing Allergic Diseases

“…The most common seafood allergens and the used method(s) for the characterization and quantification are summarized in Table 2. , 2010b;Huang et al, 2010;Kunimoto et al, 2009;Leung & Chu, 1998;Liang et al, 2008;Motoyama et al, 2007;Shibahara et al, 2009;Werner et al, 2007;Yu et al, 2010) Crab DeWitt et al, 2004;Leung et al, 1996;Leung & Chu, 1998;Motoyama et al, 2007;Mykles et al, 1998;Shibahara et al, 2009;Werr et al, 2009) To characterize seafood allergens, it is necessary to profile the complex crude extracts by separating the protein content by gel electrophoresis. This technique plays a central role in seafood allergen research, wherein it provides a powerful separation and semi-quantification information.…”