The effect of sulfamethoxazole on oxidative stress indices in zebrafish (<i>Danio rerio</i>)

Tokanová, Nina; Dobšíková, Radka; Doubková, Veronika; Blahová, Jana; Svobodová, Zdeňka; Maršálek, Petr

doi:10.1080/01480545.2018.1560465

Cited by 12 publications

(4 citation statements)

References 29 publications

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“…CMN can cause oxidative stress in zebra sh gills and produce a dose-dependent DNA damage response (Paravani, et al, 2019). The oxidative stress index of zebra sh increased 14 days after exposure to SMZ, and the cell membrane was damaged (Tokanova, et al, 2021). These studies con rm that our evidence is reliable.…”

Section: Discussionsupporting

confidence: 74%

Oxidative stress is involved in the activation of NF-κB signal pathway and immune inflammatory response in grass carp gill induced by cypermethrin and/or sulfamethoxazole

Wang

Zhao

et al. 2021

Environ Sci Pollut Res

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At present, the concentration of environmental pollutants, such as pesticides and antibiotics exposed in environment, especially in aquatic environment is increasing. These contaminants are exposed through aquatic environment to sh and ultimately accumulate in humans. Research on environmental pollutants has exploded in the past two years. However, there are still few studies on the combined effects of pesticides and antibiotics on sh, especially on sh gills. A separate analysis of the toxic effects caused by an environmental pollutant cannot fully show the real situation. In this paper, cypermethrin (CMN) and sulfamethoxazole (SMZ) were analyzed and found that there was a strong correlation between the pathways affected by the rst 30 genes regulated by CMN and SMZ respectively. Therefore, the toxic effects of CMN (0.651 μg/L) and/or SMZ (0.3 μg/L) on grass carp gill were studied in this paper. Histopathology, quantitative real-time PCR and other methods were used to detect the tissue morphology, oxidative stress level, in ammation and apoptosis-related indicators of the sh gills after exposure 42 days. It was found that compared with the single exposure (SMZ/CMN) group, the combined exposure (MIX) group had a more pronounced oxidative stress index imbalance. At the same time, nuclear factor-κB (NF-κB) signal pathway was activated and immuno-in ammatory reaction appeared in MIX group.This study reveals the harm of CMN and SMZ to sh, and provides a reference and basis for the rational use of pesticides and antibiotics.

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Section: Discussionsupporting

confidence: 74%

Oxidative stress is involved in the activation of NF-κB signal pathway and immune inflammatory response in grass carp gill induced by cypermethrin and/or sulfamethoxazole

Wang

Zhao

et al. 2021

Environ Sci Pollut Res

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“…Compared with (fluor)quinolones, sulphonamides mainly suppress microorganism growth and multiplication and act as a competitive inhibitor of the bacterial enzyme dihydropteroate synthetase (DPS), an enzyme involved in folate biosynthesis [ 107 ]. Tokanová et al [ 90 ] have showed that the exposure of zebrafish to sulfamethoxazole (50–500 μg/L) for 14 days has no significant impact on the antioxidant enzyme activity (e.g., glutathione peroxidase, glutathione reductase and glutathione S-transferase) compared to the control group. In the same study, a slight increase in lipid peroxidation has been observed for all tested concentrations.…”

Section: Effects Of Individual Antibiotics In Zebrafishmentioning

confidence: 99%

Zebrafish as a Screening Model to Study the Single and Joint Effects of Antibiotics

et al. 2021

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The overuse of antibiotics combined with the limitation of wastewater facilities has resulted in drug residue accumulation in the natural environment. Thus, in recent years, the presence of antibiotic residues in the environment has raised concerns over the potential harmful effects on ecosystems and human health. The in vivo studies represent an essential step to study the potential impact induced by pharmaceutical exposure. Due to the limitations of traditional vertebrate model systems, zebrafish (Danio rerio) has recently emerged as a promising animal model to study the toxic effects of drugs and their therapeutic efficacy. The present review summarizes the recent advances made on the toxicity of seven representative classes of antibiotics, namely aminoglycosides, β-lactams, macrolides, quinolones, sulfonamides, tetracyclines and polyether antibiotics, in zebrafish, as well as the combined effects of antibiotic mixtures, to date. Despite a significant amount of the literature describing the impact of single antibiotic exposure, little information exists on the effects of antibiotic mixtures using zebrafish as an animal model. Most of the research papers on this topic have focused on antibiotic toxicity in zebrafish across different developmental stages rather than on their efficacy assessment.

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“…A lack of antioxidant defense in response to antibiotic exposure has been reported previously in zebrafish. Exposure to SMX at 50–500 µg/L for 14 days did not alter the activity of antioxidant enzymes in zebrafish [ 37 ]. Another study reported evidence of oxidative stress in the brain of Cyprinus carpio exposed to 200 µg/L of SMX in a 28-day chronic exposure [ 20 ].…”

Section: Discussionmentioning

confidence: 99%

Sulfamethoxazole (SMX) Alters Immune and Apoptotic Endpoints in Developing Zebrafish (Danio rerio)

Iftikhar

Konig

English

et al. 2023

Toxics

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Sulfamethoxazole (SMX) is a broad-range bacteriostatic antibiotic widely used in animal and fish farming and is also employed in human medicine. These antibiotics can ultimately end up in the aquatic ecosystem and affect non-target organisms such as fish. To discern the effect of SMX on developing zebrafish embryos and larvae, we investigated a broad range of sub-lethal toxicity endpoints. Higher concentrations of SMX affected survivability, caused hatch delay, and induced malformations including edema of the yolk sac, pericardial effusion, bent tail, and curved spine in developing embryos. Lower levels of SMX provoked an inflammatory response in larvae at seven days post fertilization (dpf), as noted by up-regulation of interferon (ifn-γ) and interleukin 1β (il-1β). SMX also increased the expression of genes related to apoptosis, including BCL2-Associated Agonist of Cell Death (bad) and BCL2 Associated X, Apoptosis Regulator (bax) at 50 µg/L and decreased caspase 3 (casp3) expression in a dose-dependent manner. SMX induced hyperactivity in larval fish at 500 and 2500 µg/L based upon the light/dark preference test. Collectively, this study revealed that exposure to SMX can disrupt the immune system by altering host defense mechanisms as well as transcripts related to apoptosis. These data improve understanding of antibiotic chemical toxicity in aquatic organisms and serves as a baseline for in-depth environmental risk assessment of SMX and antibiotics.

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The effect of sulfamethoxazole on oxidative stress indices in zebrafish (Danio rerio)

Cited by 12 publications

References 29 publications

Oxidative stress is involved in the activation of NF-κB signal pathway and immune inflammatory response in grass carp gill induced by cypermethrin and/or sulfamethoxazole

Oxidative stress is involved in the activation of NF-κB signal pathway and immune inflammatory response in grass carp gill induced by cypermethrin and/or sulfamethoxazole

Zebrafish as a Screening Model to Study the Single and Joint Effects of Antibiotics

Sulfamethoxazole (SMX) Alters Immune and Apoptotic Endpoints in Developing Zebrafish (Danio rerio)

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