The effect of temperature on cellular reactions of Crassostrea virginica to the injection of avian erythrocytes

Feng, S. Y.; Feng, J. S.

doi:10.1016/0022-2011(74)90068-8

Cited by 40 publications

(11 citation statements)

References 17 publications

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“…Indeed, the complete blood collection of an experimental oyster (10 g of meat) routinely provides 10 6 hemocytes (Rolland et al, 2012), which is a conservative value because the proportion of blood cells infiltrating the oyster tissues is unknown. In contrast, the hemocyte half-life was determined to be 22 days for the related eastern oyster, C. virginica (Feng and Feng, 1974), a value consistent with the 28 days found for another bivalve, Mercenaria mercenaria (McIntosh and Robinson, 1999). Based upon these values, the lower range for the daily loss of hemocytes can be estimated at 22.000 h day −1 for a medium-sized individual.…”

Section: Research Articlesupporting

confidence: 69%

Adult somatic progenitor cells and hematopoiesis in oyster

Jèmaà

Morin

Cavelier

et al. 2014

Journal of Experimental Biology

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Long-lived animals show a non-observable age-related decline in immune defense, which is provided by blood cells that derive from self-renewing stem cells. The oldest living animals are bivalves. Yet, the origin of hemocytes, the cells involved in innate immunity, is unknown in bivalves and current knowledge about mollusk adult somatic stem cells is scarce. Here we identify a population of adult somatic precursor cells and show their differentiation into hemocytes. Oyster gill contains an as yet unreported irregularly folded structure (IFS) with stem-like cells bathing into the hemolymph. BrdU labeling revealed that the stem-like cells in the gill epithelium and in the nearby hemolymph replicate DNA. Proliferation of this cell population was further evidenced by phosphorylated-histone H3 mitotic staining. Finally, these small cells, most abundant in the IFS epithelium, were found to be positive for the stemness marker Sox2. We provide evidence for hematopoiesis by showing that co-expression of Sox2 and Cu/Zn superoxide dismutase, a hemocyte-specific enzyme, does not occur in the gill epithelial cells but rather in the underlying tissues and vessels. We further confirm the hematopoietic features of these cells by the detection of Filamin, a protein specific for a subpopulation of hemocytes, in large BrdU-labeled cells bathing into gill vessels. Altogether, our data show that progenitor cells differentiate into hemocytes in the gill, which suggests that hematopoiesis occurs in oyster gills.

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Section: Research Articlesupporting

confidence: 69%

Adult somatic progenitor cells and hematopoiesis in oyster

Jèmaà

Morin

Cavelier

et al. 2014

Journal of Experimental Biology

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“…For example, an elevated temperature has been reported to increase phagocytosis [31] and haemocyte activity (the ability of haemocyte to adhere the fluorescent beads) in the American oyster Crassostrea virginica [32]. Steenbergen et al [33] reported that phagocytosis of the American lobster Homarus americanus was higher at 20 (C than that at 22 (C. Smith and Chisholm [27] reported that antibacterial activity of the shore crab C. maenas against Planococcus citreus was significantly higher at 6 (C as compared to the crab at 13 and 19 (C, which correlated well with higher THC and phenoloxidase activity.…”

Section: Discussionmentioning

confidence: 99%

Change in water temperature on the immune response of Taiwan abalone Haliotis diversicolor supertexta and its susceptibility to Vibrio parahaemolyticus

Cheng

Hsiao

Hsu

et al. 2004

Fish & Shellfish Immunology

177

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“…For example, elevated temperature has been reported to increase phagocytosis (Feng & Feng 1974) and hemocyte activity (determined as the ability of hemocytes to adhere to fluorescent beads) in the American oyster Crassostrea virginica (Foley & Cheng 1975). Phagocytic activity and clearance efficiency of Vibrio parahaemolyticus decreased in Haliotis diversicolor supertexta following 24 h exposure to 0.96, 2.95, 5.03 and 10.16 mg l -1 nitrite-N.…”

Section: Discussionmentioning

confidence: 99%