“…In principle, individual taxa should be represented in the eDNA pool in proportion to their biomass if DNA sloughing and degradation rates are similar across taxa. Because eDNA supply, transport, and degradation dynamics are poorly understood (Andruszkiewicz, Sassoubre, & Boehm, ; De Souza, Godwin, Renshaw, & Larson, ; Deiner & Altermatt, ; Dell'Anno & Corinaldesi, ; Jane et al, ; Jerde et al, ; Jo et al, ; Kelly, Gallego, & Jacobs‐Palmer, ; Pilliod, Goldberg, Arkle, & Waits, ; Shogren et al, ; Turner, Uy, & Everhart, ), researchers have focused on the development and critical assessment of a variety of eDNA methods that have different strengths and weaknesses. At one end of the analytical spectrum is quantitative PCR (qPCR), a highly precise and sensitive way to monitor template eDNA abundance in real‐time PCR reactions (Murray, Coghlan, & Bunce, ; Taberlet et al, ).…”