Indonesia has The National Energy Policy based on the principle of fairness, sustainability, and environmentally friendly, and the creation of national energy security. Start from early 2015, mandatory of using biofuel as a substitution to fuel or blend on subvention fuel, nonsubvention fuel, industrial, commercial, and power plant sector is implemented by the Government of Indonesia on Ministerial Energy and Mineral Resources Regulation 12/2015. As a biofuel, bioethanol can be used as a fuel substitution for gasoline. Bioethanol is a natural product and manufactured by the fermentation of plants containing sugar and starch. Characteristic physical and chemical of bioethanol near to gasoline. The objective of this research was to determine the effect of bioethanol-gasoline blended fuels on the characteristic physical and chemical. The experimental test was using gasoline with octane number 88, 92, 98, and 5%, 10%, 15%, 20% bioethanol blend. The effect of the bioethanol-gasoline blend on physical and chemical properties is increased research octane number, oxygen content, vapor pressure, and to decrease the heating value. The one of the important parameter for combustion process on gasoline 88 is the final boiling point temperature, with bioethanol blend of 20% can decrease 7% of the final boiling point value.