The Effect of Zbxz23ir-21 NANO(nanomaterials) Delivery Vector on Apoptosis and PTEN(phosphatase and tensin homolog deleted on chromosome ten)/PI3K(Intracellular phosphatidylinositol kinase)/AKT(related to the A and C kinase) in Children with CHOLESTEATOMA in Middle Ear

Abstract

Cholesteatoma of the middle ear is a kind of cystic disease with clear boundary formed by the abnormal growth of keratosquamous epithelium in temporal bone. Cholesteatoma otitis caused by it is a common disease in otorhinolaryngology. The EPR effect promotes the selective distribution of macromolecular substances in tumor tissues, which can increase drug efficacy. The purpose of this paper is to prepare and deliver the mir34a small molecule regulator, rubine, by nanotechnology, and to deliver it to the cells successfully. It can passively target tumor tissue through EPR effect, and play its regulatory role on miR-34a, thus inhibiting the growth of cholesteatoma cells. The effects of nano delivery on apoptosis and PIEN/P13K/AKt of children with middle ear choledochoma were tested in this paper. The experimental results were conducted on cholesteatoma cells as cell lines and balb/c nude mice as experimental objects. The expression of PTEN/PI3K/AKT in experimental group and control group was detected by immunohistochemistry. Apoptosis was discussed by cell activity detection. The physical and chemical properties, encapsulation efficiency, drug release ability in vitro and antitumor activity of nanoparticles in vitro and in vivo were studied. The results of cell level experiments in vitro showed that free RUBINE caused about 15% apoptosis, which was not different from RC NPs. The results showed that the nanoparticles could improve the expression of miR-34 in the cells, and then regulate the expression of Bcl-2, Cdk6 and CyclinD1, and play the inhibitory effect of miR-34a on the proliferation and migration of tumor cells.

Micro RNAs and Circular RNAs in Different Forms of Otitis Media

“…Overexpression of miR-34a can promote apoptosis and prevent cell proliferation and migration. The study thus confirmed that the expression of miR-34 may be enhanced by nanoparticles and thereby influence CyclinD1, Bcl-2, and Cdk6 [ 30 ].…”

“…An experimental study using nanotechnology conducted by Zheng et al represents an important step forward in the practical use of miRNAs in treatment [ 30 ]. Based on AMEC tissue, the authors prepared and delivered rubine, a regulator of miR-34a, into cells.…”

Expression and Regulatory Mechanisms of MicroRNA in Cholesteatoma: A Systematic Review

“…In total, 118 articles were retrieved through the database search. After applying the search strategy described above, a total of 18 articles were identified that met all inclusion criteria [30][31][32][33][34][35][36][37][38][39][40][41][42][43][44][45][46][47]. A flow diagram of the detailed study selection process is presented in Figure 1.…”

Research Progress on Non-coding RNAs in Cholesteatoma of the Middle Ear

“…In cholesteatoma cells and a BALB/C nude mouse model, Zheng et al [ 28 ] found lower miR34a expression in cholesteatoma cells than in external ear canal skin tissue using quantitative real-time polymerase chain reaction (qRT-PCR). They also successfully prepared and delivered the mir34a small molecule regulator, rubine, by nanotechnology, into the cells.…”

“…For example, Tay et al [ 27 ] found that vesicle-associated membrane protein-associated protein A ( VAPA ) and CCR4-NOT transcription complex subunit 6-like ( CNOT6L ) mRNAs have the same MREs as PTEN and can act as ceRNA to competitively bind miRNAs, thereby indirectly altering PTEN (phosphatase and tensin homolog deleted on chromosome 10) abundance and suppressing tumorigenesis. This article reviews the research on the roles of these three types of ncRNAs in cholesteatoma, in accordance with the ceRNA hypothesis ( Table 1 ) [ 28 – 42 ].…”