The effectiveness of hydroxyapatite-beta tricalcium phosphate incorporated into stem cells from human exfoliated deciduous teeth for reconstruction of rat calvarial bone defects

Silva, Alexandre Augusto Ferreira da; Rinco, Ugo Guilherme Roque; Jacob, Ricardo Garcia Mureb; Sakai, Vivien Thiemy; Mariano, Ronaldo Célio

doi:10.1007/s00784-021-04038-9

Cited by 9 publications

(6 citation statements)

References 50 publications

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“…SHED did not show more intense inflammatory infiltrate than the control or BGM groups, indicating its low immunogenicity. Another studies also reported that SHED incorporated into poly-(lactic-co-glycolic acid)-bioactive glass composite scaffolds or HA-beta TCP successfully regenerated new bones in rat calvarial bone defects with a mild chronic inflammatory infiltrate that were similar to that of the control groups (Kunwong et al, 2021;da Silva et al, 2022).…”

Section: Discussionmentioning

confidence: 88%

Easily attainable and low immunogenic stem cells from exfoliated deciduous teeth enhanced the in vivo bone regeneration ability of gelatin/bioactive glass microsphere composite scaffolds

Xiong

Zhao

Peng

et al. 2022

Front. Bioeng. Biotechnol.

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Repair of critical-size bone defects remains a considerable challenge in the clinic. The most critical cause for incomplete healing is that osteoprogenitors cannot migrate to the central portion of the defects. Herein, stem cells from exfoliated deciduous teeth (SHED) with the properties of easy attainability and low immunogenicity were loaded into gelatin/bioactive glass (GEL/BGM) scaffolds to construct GEL/BGM + SHED engineering scaffolds. An in vitro study showed that BGM could augment the osteogenic differentiation of SHED by activating the AMPK signaling cascade, as confirmed by the elevated expression of osteogenic-related genes, and enhanced ALP activity and mineralization formation in SHED. After implantation in the critical bone defect model, GEL/BGM + SHED scaffolds exhibited low immunogenicity and significantly enhanced new bone formation in the center of the defect. These results indicated that GEL/BGM + SHED scaffolds present a new promising strategy for critical-size bone healing.

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Section: Discussionmentioning

confidence: 88%

Easily attainable and low immunogenic stem cells from exfoliated deciduous teeth enhanced the in vivo bone regeneration ability of gelatin/bioactive glass microsphere composite scaffolds

Xiong

Zhao

Peng

et al. 2022

Front. Bioeng. Biotechnol.

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“…A different investigation showed that growing SHEDs on scaffolds with osteogenic culture medium for a week in vitro before grafting into rat calvarial CSDs, enhanced bone regeneration. 249 This finding suggests that predifferentiation of SHED cells in vitro before grafting accelerates bone regeneration. In a clinical study, autologous SHEDs grown on Bio-Oss Col scaffolds and implanted into alveolar clefts in children promoted bone growth in defects, allowing the eruption of permanent teeth and causing less postoperative complications compared to clefts treated with BMP-2-loaded Col sponges or iliac crest autografts.…”

Section: Stem Cells As a Source Of Bioactive Molecules For Enhancing ...mentioning

confidence: 93%

Bioactive Scaffolds as a Promising Alternative for Enhancing Critical-Size Bone Defect Regeneration in the Craniomaxillofacial Region

Bello,

Cruz-Lebrón,

Rodríguez-Rivera

et al. 2023

ACS Appl. Bio Mater.

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Reconstruction of critical-size bone defects (CSDs) in the craniomaxillofacial (CMF) region remains challenging. Scaffold-based bone-engineered constructs have been proposed as an alternative to the classical treatments made with autografts and allografts. Scaffolds, a key component of engineered constructs, have been traditionally viewed as biologically passive temporary replacements of deficient bone lacking intrinsic cues to promote osteogenesis. Nowadays, scaffolds are functionalized, giving rise to bioactive scaffolds promoting bone regeneration more effectively than conventional counterparts. This review focuses on the three approaches most used to bioactivate scaffolds: (1) conferring microarchitectural designs or surface nanotopography; (2) loading bioactive molecules; and (3) seeding stem cells on scaffolds, providing relevant examples of in vivo (preclinical and clinical) studies where these methods are employed to enhance CSDs healing in the CMF region. From these, adding bioactive molecules (specifically bone morphogenetic proteins or BMPs) to scaffolds has been the most explored to bioactivate scaffolds. Nevertheless, the downsides of grafting BMP-loaded scaffolds in patients have limited its successful translation into clinics. Despite these drawbacks, scaffolds containing safer, cheaper, and more effective bioactive molecules, combined with stem cells and topographical cues, remain a promising alternative for clinical use to treat CSDs in the CMF complex replacing autografts and allografts.

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“…Titanium prostheses are also widely used in clinical practice . Poly( N -isopropylacrylamide), polyethylene, polypropylene, and polyetherketones , are used as polymer synthetic materials as well as poly(methyl methacrylate) and cross-linked poly(acrylic acid). − Biodegradable materials used for temporary filling of small defects and cavities include polycyanoacrylates, bioceramics and bioglasses, , hydroxyapatite, , polysaccharides, , hyaluronic acid, and biodegradable polyesters. , Synthetic grafts are easy to manufacture, and their composition and properties can be varied, however, their biological performance and mechanical properties are still limited compared to bone grafts. Although the volume of synthetic materials used for bone replacement is increasing at a record pace, allografts and xenomaterials still prevail in modern clinical practice. , …”

Section: Introductionmentioning

confidence: 99%

Gel Based on Hydroxyethyl Starch with Immobilized Amikacin for Coating of Bone Matrices in Experimental Osteomyelitis Treatment

Luss,

Kushnerev,

Vlaskina

et al. 2023

Biomacromolecules

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A polysaccharide gel containing covalently bound amikacin, a broad-spectrum antibiotic, was produced by using epichlorohydrin-activated hydroxyethyl starch (HES). The structure of the polymers was analyzed by 13 C and 1 H nuclear magnetic resonance ( 13 C NMR and 1 H NMR) and matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. The sites of covalent attachment of amikacin to the epoxypropyl substituent and the HES backbone were determined. The antibacterial activity of the polymer was evaluated in vitro using the agar well diffusion method with the Staphylococcus aureus P209 strain. It was demonstrated that the polymer retained activity in the presence of bacterial amylase, which is released upon bacterial attack. The gel was applied for coating pores and surfaces of a biocomposite material based on a xenogenic bovine bone matrix. In vivo experiments showed the effectiveness of utilizing amikacin-containing biocomposite bone-substitute materials in the treatment of experimental osteomyelitis in rats using objective histological control and X-ray tomography.

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The effectiveness of hydroxyapatite-beta tricalcium phosphate incorporated into stem cells from human exfoliated deciduous teeth for reconstruction of rat calvarial bone defects

Cited by 9 publications

References 50 publications

Easily attainable and low immunogenic stem cells from exfoliated deciduous teeth enhanced the in vivo bone regeneration ability of gelatin/bioactive glass microsphere composite scaffolds

Easily attainable and low immunogenic stem cells from exfoliated deciduous teeth enhanced the in vivo bone regeneration ability of gelatin/bioactive glass microsphere composite scaffolds

Bioactive Scaffolds as a Promising Alternative for Enhancing Critical-Size Bone Defect Regeneration in the Craniomaxillofacial Region

Gel Based on Hydroxyethyl Starch with Immobilized Amikacin for Coating of Bone Matrices in Experimental Osteomyelitis Treatment

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