﻿The effectiveness of the use of polypeptide drugs and their effect on the metabolic parameters of oral fluid in patients with generalized periodontitis in depending on blood type

Bandrivsky, Yurii; Bandrivska, Orysia; Malko, Nataliia; Посоленик, Л. Я.; Vydoinyk, Oksana; Iskiv, Maryana

doi:10.3897/pharmacia.69.e82421

Cited by 8 publications

(3 citation statements)

References 18 publications

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“…The amount of absorbed dye (as a percentage) was calculated from the difference between the optical density of the initial dye solution and the dye solution after incubation with erythrocytes. [16] The results were statistically analyzed by means of non-parametric indices methods using the STATISTICA 10.0 software (Statsoft, USA). The reliability of the differences in values between independent quantitative values was determined with a normal distribution according to the Mann-Whitney U criterion.…”

Section: Methodsmentioning

confidence: 99%

Endogenous intoxication in development of experimental periodontitis of bacterial-immune genesis

Demkovych¹

2023

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Introduction: Excessive lipoperoxidation is accompanied by accumulation of peroxidation products and depletion of antioxidant re-serves, which cause hyperenzymemia and the accumulation of toxic substances. The level of endotoxicosis is determined by the content of hydrophilic and hydrophobic products in the blood.  Aim: To define the pathogenetic role of endogenous intoxication in the dynamics of development of experimental periodontitis of bacterial-immune genesis.  Materials and methods: The experiment was conducted on rats. The animals were divided into three groups: group 1 – control group/intact; group 2 – model periodontitis at 7 days; group 3 – model periodontitis at 30 days. The experimental bacterial-immune periodon-titis was induced by injection into the tissue of the periodontal complex of the microorganisms’ mixture diluted with egg’s albumin. For the study, we selected the blood serum in which the content of middle molecular weight molecules and erythrocyte intoxication index were determined. The results were statistically analyzed by means of non-parametric indices methods.  Results: The development of generalized periodontitis is characterized by the occurrence of oxidative stress, which leads to violation of the metabolism in the mucous membrane of the oral cavity resulting in the accumulation of toxic products and the development of endogenous intoxication. The results show that the content of middle molecular weight molecules (aromatic amino and chain amino acids) determined on day 7 of the experiment was 1.11 times higher than that of the control group (p<0.01) and by 1.16 times (p<0.01), respectively. Comparing the levels of the above hydrophilic components of endogenous intoxication at 30 days of experimental peri-odontitis, we found a probable increase in these indicators compared with those at 7 days of the experiment. Studying the level of eryth-rocyte intoxication index, we found that at 7 days of experimental periodontitis this index was 1.28 times higher (p<0.01) than that of the intact group and continued to increase at 30 days. Conclusions: The dynamics of experimental periodontitis of bacterial-immune genesis show that the highest rates of endogenous intoxication are found in the late stages of the dynamics of the inflammatory process in the periodontium, namely at 30 days of the experiment, which may indicate chronic inflammation.

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Section: Methodsmentioning

confidence: 99%

Endogenous intoxication in development of experimental periodontitis of bacterial-immune genesis

Demkovych¹

2023

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“…Today, one of the current areas for therapeutic and prophylactic effects on periodontal tissues is the study of the mechanism of action of peptide bioregulators of multicellular systems, in particular, short low molecular weight synthesized peptides of selective action. The unique sequence of amino acids in peptides is a certain information stimulus-carrier for the damaged cell, which regulates metabolic processes, enhances protein synthesis in the periodontium, increases antioxidant activity, improves blood microcirculation and accelerates the recovery of periodontal tissues and its functions [18,19].…”

Section: R E C O O P H S T a S S O C I A T I O N R E C O O P H S T A ...mentioning

confidence: 99%

Changes in the activity of phosphatases, calcium and phosphorus in rats with the different courses of gingivitis under correction by anti-inflammatory gel

Avdeev¹,

Drevnitska²,

Gevkaliuk³

et al. 2022

Ukr.Biochem.J.

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The aim of the study was to evaluate changes in the activity of acid and alkaline phosphatases, calcium and phosphorus levels in rats with different courses of experimental gingivitis upon treatment with anti-inflammatory gel with Neovitin and peptide complexes. The experiment was conducted on 100 white nonlinear male rats aged 5-6 months divided into 10 groups: 1 control and 9 – with different courseі of gingivitis. The activity of alkaline and acid phosphatase (ALP, ACP), the levels of calcium (Ca) and phosphorus (P) in rat blood serum and gingiva supernatant were determined. It was found that upon gingivitis, the activity of ALP in blood serum decreased and in gingiva supernatant increased in all groups of animals compared to the control group. The activity of ACP in the serum decreased in hypoergic and hyperergic animal groups and increased in normergia, and in gingiva supernatant increased in all groups: by 2 times in normoergic and hypoergic animals and by 1.4 times in hyperergic. The treatment with anti-inflammatory gel normalized the activity of ALP in both serum and supernatant and decreased the ACP activity in the serum of animals in hypo- and hyperergic groups. The content of serum Ca increased in all groups, and in the supernatant of the gingiva even exceeded the control value. The content of phosphorus in the supernatant of periodontal tissues decreased. The development of the inflammatory process in the periodontium of rats with gingivitis was accompanied by changes in the activity of ACP, ALP, the content of Ca and P in the blood serum and gingival supernatant. The treatment with gel containing neovitin and peptide complexes had a more pronounced therapeutic effect in rats with unchanged reactivity of the organism. Keywords: acid phosphatase, alkaline phosphatase, anti-inflammatory gel, calcium and phosphorus levels, gingivitis

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“…Modern research has proven the dualism of the effects of thyroid hormones on bone metabolism. Metabolic disorders lead to the fact that all patients with diffuse toxic goiter, even in the early stages of the disease, have inflammatory-dystrophic changes in the periodontium [12,13].…”

Section: Introductionmentioning

confidence: 99%

Submicroscopic changes of periodontal components under experimental periodontitis combined with hypothyroidism

Shcherba,

Demkovych,

Vorobets

et al. 2023

Fiziol Zh

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Endocrine disorders are an extremely urgent medical and social problem of modern society. Changes in the thyroid gland activity cause violations of the regulation of metabolism in periodontal tissues. Expressed clinical signs of periodontal tissues inflammation directly depend on the severity and duration of disorders of the functional state of the thyroid gland. The purpose of this study was to describe the ultrastructural changes of periodontal tissues under the conditions of experimental periodontitis in combination with hypothyroidism. To model periodontitis lipopolysaccharide was injected into the gum tissue of rats. The experimental thyroid gland hypofunction was simulated using intragastrical administration of mercazolyl. To confirm the state of hypothyroidism we determined the content of free thyroxine, free triiodothyronine and thyroid-stimulating hormone. Submicroscopic studies revealed profound destructive-degenerative changes in all components of the periodontium. Changes in periodontal tissues in experimental hypothyroidism are stereotyped: dystrophic, atrophic and destructive changes in parenchymal structures, mucinous edema of the stroma, mild reparative processes. Thus, it can be concluded that during experimental periodontitis in combination with hypothyroidism, there are violations of the functioning of all the components of the vessels of the periodontal microcirculatory channel. This is manifested by damage and deformation of the blood vessel wall with desquamation of the endothelium, edema, thinning of the basement membrane, stasis, thrombosis and spasm. Also, significant destruction and lysis of fibrous structures and components of the connective tissue amorphous substance in the lamina propria of the attached and free part of gums and periodontium are characteristic.

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The effectiveness of the use of polypeptide drugs and their effect on the metabolic parameters of oral fluid in patients with generalized periodontitis in depending on blood type

Cited by 8 publications

References 18 publications

Endogenous intoxication in development of experimental periodontitis of bacterial-immune genesis

Endogenous intoxication in development of experimental periodontitis of bacterial-immune genesis

Changes in the activity of phosphatases, calcium and phosphorus in rats with the different courses of gingivitis under correction by anti-inflammatory gel

Submicroscopic changes of periodontal components under experimental periodontitis combined with hypothyroidism

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﻿The effectiveness of the use of polypeptide drugs and their effect on the metabolic parameters of oral fluid in patients with generalized periodontitis in depending on blood type

Cited by 8 publications

References 18 publications

Endogenous intoxication in development of experimental periodontitis of bacterial-immune genesis

Endogenous intoxication in development of experimental periodontitis of bacterial-immune genesis

Changes in the activity of phosphatases, calcium and phosphorus in rats with the different courses of gingivitis under correction by anti-inflammatory gel

Submicroscopic changes of periodontal components under experimental periodontitis combined with hypothyroidism

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The effectiveness of the use of polypeptide drugs and their effect on the metabolic parameters of oral fluid in patients with generalized periodontitis in depending on blood type