The effectiveness of three multi-component binding models in describing the binary competitive equilibrium adsorption of two cytochrome b5 mutants

Cano, Tony; Offringa, Natalie D.; Willson, Richard C.

doi:10.1016/j.chroma.2007.01.059

Cited by 10 publications

(2 citation statements)

References 19 publications

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“…This may indicate that different components have different retention times on HZ820 during the desorption process due to the different physical and thermodynamic characteristics of each component (Gano et al, 2007).…”

Section: Dynamic Desorption Curve On Hz820 Resinmentioning

confidence: 99%

Enrichment and Purification of Bitespiramycin Using Macroporous Resin

Zhu

Chen

et al. 2012

Chemical Engineering Communications

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Bitespiramycin was purified by selective adsorption. The adsorption and desorption properties of bitespiramycin on six different macroporous resins (HZ806, HZ816, HZ820, HZ830, XAD16, and SP207) were compared systematically. According to the adsorption capacity and selectivity towards 4 00 -O-isovalerylspiramycin, HZ820 was chosen as the most suitable resin for bitespiramycin purification. The equilibrium data on HZ820 in a batch system were well described by Langmuir and Freundlich models. The film and pore diffusion model was successfully measured in batch adsorption kinetics. Dynamic adsorption and desorption experiments were also performed using a packed column of HZ820 to optimize the separation process of 4 00 -O-isovalerylspiramycin from aqueous solution. After being treated with HZ820, the 4 00 -O-isovalerylspiramycin content increased from 80.4% to 91.7%, and the 4 00 -O-isovalerylspiramycin III content increased from 41.7% to 64.4%.

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Section: Dynamic Desorption Curve On Hz820 Resinmentioning

confidence: 99%

Enrichment and Purification of Bitespiramycin Using Macroporous Resin

Zhu

Chen

et al. 2012

Chemical Engineering Communications

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“…Proteins that are adsorbed to a surface can denature, and may change in α-helix content, β-sheet content, and structural rigidity [15, 16]. In addition, studies on the kinetics of protein adsorption in a two-component system are rare, dependent on many parameters (including diffusion, components, competitive adsorption, pH, temperature, and others) and are ineffective at accurately predicting adsorption in multi-component systems [17–19]. Thus, it is difficult to predict the concentration and conformation of adsorbed proteins in a cell culture environment.…”

Section: Biological Functionalizationmentioning

confidence: 99%

Surface functionalization and dynamics of polymeric cell culture substrates

Krutty

Schmitt

Gopalan

et al. 2016

Current Opinion in Biotechnology

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The promise of growing tissues to replace or improve the function of failing ones, a practice often referred to as regenerative medicine, has been driven in recent years by the development of stem cells and cell lines. Stem cells are typically cultured outside the body to increase cell number or differentiate the cells into mature cell types. In order to maximize the regenerative potential of these cells, there is a need to understand cell-material interactions that direct cell behavior and cell-material dynamics. Most synthetic surfaces used for growth and differentiation of cells in the lab are impractical and cost prohibitive in clinical labs. This review focuses on the modification of low cost polymer substrates that are already widely used for cell culture so that they may be used to control and understand cell-material interactions. In addition, we discuss the ability of cells to exert dynamic control over the microenvironment leading to a more complex, less controlled surface.

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Single and binary adsorption of proteins on ion‐exchange adsorbent: The effectiveness of isothermal models

Liang

Fieg

Shi

et al. 2012

J of Separation Science

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Simultaneous and sequential adsorption equilibria of single and binary adsorption of bovine serum albumin and bovine hemoglobin on Q Sepharose FF were investigated in different buffer constituents and initial conditions. The results in simultaneous adsorption showed that both proteins underwent competitive adsorption onto the adsorbent following greatly by protein-surface interaction. Preferentially adsorbed albumin complied with the universal rule of ion-exchange adsorption whereas buffer had no marked influence on hemoglobin adsorption. Moreover, an increase in initial ratios of proteins was benefit to a growth of adsorption density. In sequential adsorption, hemoglobin had the same adsorption densities as single-component adsorption. It was attributed to the displacement of preadsorbed albumin and multiple layer adsorption of hemoglobin. Three isothermal models (i.e. extended Langmuir, steric mass-action, and statistical thermodynamic (ST) models) were introduced to describe the ion-exchange adsorption of albumin and hemoglobin mixtures. The results suggested that extended Langmuir model gave the lowest deviation in describing preferential adsorption of albumin at a given salt concentration while steric mass-action model could very well describe the salt effect in albumin adsorption. For weaker adsorbed hemoglobin, ST model was the preferred choice. In concert with breakthrough data, the research further revealed the complexity in ion-exchange adsorption of proteins.

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The effectiveness of three multi-component binding models in describing the binary competitive equilibrium adsorption of two cytochrome b5 mutants

Cited by 10 publications

References 19 publications

Enrichment and Purification of Bitespiramycin Using Macroporous Resin

Enrichment and Purification of Bitespiramycin Using Macroporous Resin

Surface functionalization and dynamics of polymeric cell culture substrates

Single and binary adsorption of proteins on ion‐exchange adsorbent: The effectiveness of isothermal models

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