The effects of ammonium metavanadate on biochemical hormonal, haematological and histopathological parameters of the female wistar rats

Abstract

Summary:The effects of different doses of Ammonium metavanadate on the biochemical, haematological, hormonal and histopathological parameters of stilbesterol treated female Wistar rats were investigated. Ammonium metavanadate in the dose-range 0-6mg/kg caused a bi-phasic and time-dependent response on the acid (total and prostate) phosphatase.. Furthermore ammonium metavanadate caused a dose-dependent inhibition of the serum alkaline phosphatases. The maximal inhibitory response at 5mg/kg of ammonium metavanadate was 40.0 ± 1.69 compared to 65.0 ± 0.94 control values. Ammonium metavanadate also caused a positively correlated biphasic response in the serum female hormonal concentrations with an initial increase, followed by a time-dependent decrease in the serum values of luteinizing (LH), follicle stimulating hormone (FSH), prolactin . Furthermore ammonium metavanadate also caused time-and dose-dependent effects on the haematological parameters. The effects were biphasic-increase within 72 hours and a reduction in the values of haemoglobin and packed cell volume within 7-28 days. The white blood count and lymphocyte counts were also reduced significantly at P ≤ 0.05. However the neutrophil counts were increased dose-and time-dependently. Finally, ammonium metavanadate caused a dose-dependent destruction of the liver and female reproductive organs namely the uterus, ovary and fallopian tubes. These were characterized by necrosis, oedema, eosinophilic deposits and vacuolation. These results may be explained by the oxidative effects caused by the free oxygen (O 2 ) radical generated by the metavanadate ions.

Testicular Mineralization in KK-A^y Mice Treated with an Oxovanadium Complex

“…There are no reports of either mineralization or degeneration/necrosis of the seminiferous tubules in previous studies 2 , 3 , 4 , 5 , 6 . Vanadium concentrations in the testes with slight to severe mineralization were higher than those in the testes without mineralization, and the testes with mineralization also showed higher calcium and iron concentrations.…”

“…The toxic effects of inorganic V 5+ salts in diabetes model rats include severe diarrhea in life. Histopathologically, hepatocellular necrosis, fatty change and vacuolation, necrosis of renal tubules and necrosis of mucosal epithelial cells in the small intestine have been reported as the toxicities 2 , 3 , 4 , 5 . In addition, testicular toxicity that seems to be related to vanadium-induced oxidative stress during spermatogenesis has been reported 6 .…”

Changes in Ovarian and Uterine Morphology and Estrous Cycle in CD-1 Mice After Vanadium Inhalation

“…26 Metals such as chromium, cadmium, and iron can alter the hormonal axis indirectly through the generation of oxidative stress. [27][28][29] Obianime et al 30 observed a decrease in the blood levels of Luteinizing Hormone (LH), Follicle Stimulating Hormone (FSH), and prolactin in rats, following intraperitoneal administration of ammonium metavanadate; this decrease in LH and FSH caused by vanadium could explain the low levels of estrogen and progesterone in the blood of exposed mice. In normal mice, the hormones synthesized in the ovary are regulated by the pulses of LH and FSH produced in the anterior pituitary gland; it is possible that vanadium is also interfering in the liberation of these hormones by this gland; however, this hypothesis needs further research to explore this possible explanation.…”

Cadmium‐induced neurodegeneration and activation of noncanonical sonic hedgehog pathway in rat cerebellum

“…These free radical scavengers protect the body from such damages only to an extent. Cadmium, like all other heavy metals, has been reported to induce oxidative stress . Oxidative stress contributes to DNA damage and impaired DNA repairing .…”