The Effects of Buckwheat Leaf and Flower Extracts on Antioxidant Status in Mouse Organs

Sadauskienė, Ilona; Liekis, Arūnas; Bernotienė, Rasa; Šulinskienė, Jurgita; Kašauskas, Artūras; Žekonis, Gediminas

doi:10.1155/2018/6712407

Cited by 15 publications

(16 citation statements)

References 25 publications

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“…Cannabis sativa L. produces multiple chemical compounds of different biogenetic classes [ 15 ], and many studies have demonstrated the antioxidant activity of Cannabis in vitro [ 19 , 22 ]. The phenolic compounds are mainly responsible for the antioxidant activity, while hemp seeds due to their active compounds, have health benefits such as lowering the cholesterol level in the blood or alleviating constipation and are also useful in the treatment of cardiovascular diseases [ 11 , 23 ].…”

Section: Discussionmentioning

confidence: 99%

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The Effects of Cannabis sativa L. Extract on Oxidative Stress Markers In Vivo

Kubilienė

Mickutė

Baranauskaitė

et al. 2021

Life

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In recent decades, a lot of attention has been paid to Cannabis sativa L. due to its useful applications, including in fibers, oil, food for humans and animals, and therapeutics. The present study aimed to determine antioxidant activity of cannabinoids in Cannabis sativa L. in vivo, evaluating the possible antioxidative effect of Cannabis sativa L. extract (CE) on malondialdehyde (MDA) and glutathione (GSH) concentrations as well as on catalase (CAT) activity in BALB/c mice. In total, 40 mice were divided into five equal groups: the aluminum group (7.5 mg AlCl3/kg/d (0.15 LD50), the saline group, the 10% ethanol group (an appropriate amount of the solution for mouse weight), the CE group (1.6 mg CE/g/day), and the aluminum-CE group (7.5 mg AlCl3 plus 1.6 mg CE/g/day). The results of the study showed that CE significantly decreased (by 26.81%, p < 0.05) the concentration of GSH in blood of the mice and the concentration of MDA in the brain (by 82.12%) and liver (by 53.5%) of the mice compared to the respective concentrations in the AlCl3 group. CE significantly (p < 0.05) increased CAT activity in the brain (by 64.79%) and liver (by 72.37%) of the mice after the AlCl3-induced prooxidant effect. The results showed the antioxidant activity of cannabidiolic acid (CBDA) in vitro. The findings in vivo indicate that Cannabis sativa L. is a good source of natural antioxidants and can be used in the management of oxidative stress.

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Section: Discussionmentioning

confidence: 99%

“…It can form a complex with thiobarbituric acid (TBA), which can be detected via spectrophotometry. The results are expressed as nmol/g of the wet weight of the tissue [ 22 ]. During the evaluation, 3 mL of 1% phosphoric acid were mixed with 0.5 mL of brain or liver homogenate and 1 mL of 0.6% TBA solution.…”

Section: Methodsmentioning

confidence: 99%

The Effects of Cannabis sativa L. Extract on Oxidative Stress Markers In Vivo

Kubilienė

Mickutė

Baranauskaitė

et al. 2021

Life

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“…Protective effects of rutin against liver injury induced by biliary obstruction and type 2 diabetes were also demonstrated in animal models [ 46 , 47 ]. Recently, Sadauskiene et al also demonstrated that the administration of buckwheat flower and leaf extracts had a significant effect on the antioxidant enzyme activity in the brain and liver of mice [ 48 ]. Furthermore, the serum antioxidant activity of buckwheat honey was reported in healthy humans [ 49 ].…”

Section: Resultsmentioning

confidence: 99%

Pharmacokinetics and Protective Effects of Tartary Buckwheat Flour Extracts against Ethanol-Induced Liver Injury in Rats

2020

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The grains of Tartary buckwheat (Fagopyrum esculentum) are traditionally consumed on a daily basis and are used in the preparation of diverse processed foods owing to the high concentration of rutin, an antioxidant compound. However, rutin is highly concentrated in hull and bran, but not in edible flour fractions. Rutin-enriched TB flour extracts (TBFEs) were obtained by hydrothermal treatment (autoclaving, boiling, or steaming) and their pharmacokinetic profiles were evaluated following a single-dose oral administration in rats. The antioxidant and protective activities of the extracts against alcoholic liver disease (ALD) were investigated after repetitive oral administration of TBFEs for 28 days prior to ethanol ingestion. The results demonstrated that rutin-enriched TBFEs had better oral absorption and was retained longer in the bloodstream than native TBFE or standard rutin. The activities of antioxidant enzymes and intracellular antioxidant levels increased in ALD rats following TBFE treatments, especially following the administration of rutin-enriched TBFEs. The antioxidant activity of TBFEs consequently contributed toward protecting the liver against injury caused by repetitive ethanol administration, as confirmed by analyzing relative liver weight, liver injury markers, lipid peroxidation, and calcium permeability. These results suggest the promising potential of TBFEs as antioxidant-enriched functional foods for human health.

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“…Compared to common buckwheat ( Fagopyrum esculentum ) , it has a characteristically bitter flavor. In addition to its use in food, the ethanol extract of tartary buckwheat (TBE) is utilized as a component of traditional Chinese medicine [ 8 ]. The administration of TBE to mouse brain and liver was shown to reduce antioxidant enzyme levels [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

“…In addition to its use in food, the ethanol extract of tartary buckwheat (TBE) is utilized as a component of traditional Chinese medicine [ 8 ]. The administration of TBE to mouse brain and liver was shown to reduce antioxidant enzyme levels [ 8 ]. It also exerted an antibacterial effect against Propionibacterium and Staphylococci species [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

Quercetin in Tartary Buckwheat Induces Autophagy against Protein Aggregations

Ikari

Liu³

et al. 2021

Antioxidants

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Tartary buckwheat is used as an ingredient in flour and tea, as well as in traditional Chinese medicine for its antioxidant effects. Here, we found that an ethanol extract of tartary buckwheat (TBE) potently induced autophagy flux in HeLa cells by suppressing mTORC1 activity, as revealed by dephosphorylation of the mTORC1 substrates Ulk1, S6K, and 4EBP, as well as by the nuclear translocation of transcriptional factor EB. In addition to non-selective bulk autophagy, TBE also induced aggrephagy, which is defined as autophagy against aggregated proteins. Quercetin is a flavonol found at high levels in TBE. We showed that quercetin induced both non-selective bulk autophagy and aggrephagy. These effects were also observed in Huh-7 cells derived from hepatocytes. Thus, aggrephagy induction by TBE and quercetin may relieve alcoholic hepatitis, which is closely linked to the accumulation of protein aggregations called Mallory–Denk bodies.

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The Effects of Buckwheat Leaf and Flower Extracts on Antioxidant Status in Mouse Organs

Cited by 15 publications

References 25 publications

The Effects of Cannabis sativa L. Extract on Oxidative Stress Markers In Vivo

The Effects of Cannabis sativa L. Extract on Oxidative Stress Markers In Vivo

Pharmacokinetics and Protective Effects of Tartary Buckwheat Flour Extracts against Ethanol-Induced Liver Injury in Rats

Quercetin in Tartary Buckwheat Induces Autophagy against Protein Aggregations

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