The effects of dietary verbascoside on blood and liver oxidative stress status induced by a high n-6 polyunsaturated fatty acids diet in piglets12

Abstract

Twenty-four weaned female Hypor piglets (10.9 ± 0.1 kg mean BW) were used to evaluate the antioxidant effect of a natural extract, titrated in verbascoside, on blood and liver oxidative status in relation to a high intake of n-6 PUFA, inducing oxidative stress. Piglets were assigned to 1 of 3 experimental groups; the first group was fed a diet with 9% sunflower oil (T1) and the second received the sunflower oil diet supplemented with 5 mg of verbascoside/kg feed from Verbenaceae extract (Lippia spp.; T2). The third group was fed a control diet (CTR), in which an isoenergetic replacement of oil by starch was done. Blood samples were collected at the beginning and the end of the trial (30 d). At the end of the trial, the animals were slaughtered and the liver specimens were collected. Oxidative stress markers, including total antiradical activity, superoxide dismutase (SOD), glutathione peroxidase (GPX), and catalase (CAT) activities, were determined in blood samples. Alanine aminotransferase (ALT), aspartate aminotransferase (AST), and γ-glutamyl transferase (GGT) plasma levels were also evaluated. Immunohistochemistry and western blot analyses were performed in liver to evaluate heat shock protein (Hsp) 70, Hsp90, and Kupffer and Ito cell activation. Liver activities of SOD, GPX, and CAT were also determined. Total antiradical activity in blood and red blood cells were affected (P < 0.01) by dietary treatments. The n-6 PUFA supplementation at a high dosage for 30 d induced oxidative stress, decreasing total antiradical activity in blood and red blood cells (CTR vs. T1 + T2; P < 0.01) and plasma CAT activity (CTR vs. T1 + T2; P = 0.088) and increasing ALT value (CTR vs. T1 + T2; P < 0.01). Also, in liver, the CAT and GPX activities tended to be lower in pigs fed n-6 PUFA diets than pigs fed a control diet (CTR vs. T1 + T2; = 0.090 and = 0.085, respectively). The liver samples presented a normal architecture and no Ito and Kupffer cell activations were observed. In liver, the SOD activity tended to be lower in the T1 group (P = 0.064) than in the CTR and T2 groups. Moreover, the level of Hsp70 was higher (P < 0.01) in the T1 group than the CTR and T2 groups. These data suggest that the dose of dietary verbascoside partially restores the antioxidant status of the liver without affecting the systemic responses to oxidative stress induced by a high-fat diet.

Effects of dietary dehydrated lemon peel on some biochemical markers related to general metabolism, welfare and stress in gilthead seabream (Sparus aurataL.)

“…It is well known that many oxidizing agents cause the induction of Hsp and that the treatment of cells with antioxidants attenuates the response to stress (Padmini et al, ). Our results are consistent with the observations described by Di Giancamillo et al (), that showed the levels of Hsp70 were decreased in pigs fed a diet supplemented with a natural verbascoside extract, compared with pig fed a high‐fat diet. These authors suggested the role of Hsp70 in modulating hepatic oxidative stress (Di Giancamillo et al, ).…”

“…Our results are consistent with the observations described by Di Giancamillo et al (), that showed the levels of Hsp70 were decreased in pigs fed a diet supplemented with a natural verbascoside extract, compared with pig fed a high‐fat diet. These authors suggested the role of Hsp70 in modulating hepatic oxidative stress (Di Giancamillo et al, ).…”

Organic selenium supplementation improves growth parameters, immune and antioxidant status of newly received beef cattle

“…In several studies on animals, the antioxidant status has also been tested using the KRL test CTR SeY . This method is effective in evaluating the antioxidant status of animals in relation to dietary antioxidant supplementation and induced oxidative stress Di Giancamillo et al, 2015). We found that the blood total antiradicalic activity is enhanced by SeY supplementation.…”

Honey Bee Pollen in Meagre (Argyrosomus regius) Juvenile Diets: Effects on Growth, Diet Digestibility, Intestinal Traits, and Biochemical Markers Related to Health and Stress

“…Our previous study on the inclusion of dehydrated lemon peel in the diet for seabream showed a reduction of the HSP70 levels in fish fed on increased levels of bioactive compounds, due to the antioxidant composition of this resources, which is able to guarantee protection against oxidative stress [2]. Accordingly, Di Giancamillo et al [45] described a decrease of HSP70 in pigs fed a diet supplemented with a natural verbascoside extract, compared with pig fed a high-fat diet. These authors suggested a role of HSP70 in modulating hepatic oxidative stress [45].…”

“…Accordingly, Di Giancamillo et al [45] described a decrease of HSP70 in pigs fed a diet supplemented with a natural verbascoside extract, compared with pig fed a high-fat diet. These authors suggested a role of HSP70 in modulating hepatic oxidative stress [45].…”