The effects of environmental history and thermal stress on coral physiology and immunity

Abstract

Wall et al., (2018) The effects of environmental history and thermal stress on coral physiology and immunity. Marine Biology (in press) Files These include files for making maps, and plotting the environmental and biological data. All code to generate figures and perform analyses are found in the R-markdown file (.Rmd) R markdown Mydlarz-Gates_Lab_exp_2014.Rmd This is the R.markdown file containing script for data analysis and figure generation All files in repository are used in this annotated markdown file; open in R Studio. Mydlarz-Gates_Lab_exp_2014.html This is the .html output for the R.markdown file. Environmental In situ data coast_n83_shp.zip This zip file contains 6 files. These shape files are used to generate the map of Kāne'ohe Bay. To use these files, see R markdown file; place these files in a folder names 'coast_n83.shp' in directory. KBaymapdata.csv This is a file with buoys, reef names, and their respective lat/long (°) Columns: Origin is the buoy/reef name, lat is latitude, and long is longitude CRIMP1_pCO2.csv This is the pCO 2 data from CRIMP1 buoy, downloaded from NOAA PMEL Columns: Site (CRIMP.1), Time of data collection in mm/dd/yy hh:mm, pCO2.water (µatm) is partial pressure of CO 2 (carbon dioxide) in seawater, Temperature (°C), and pCO2.air (µatm) is partial pressure of CO 2 in air.

Divergent symbiont communities determine the physiology and nutrition of a reef coral across a light-availability gradient

“…Ash-free dry weight (AFDW) (i.e., total host + symbiont biomass) of holobiont tissues was quantified as the difference between dried (60°C, 24 h) and combusted masses (450°C, 4 h). All physiological metrics (cell densities, chlorophyll concentrations, total biomass) were standardized to coral surface area, determined using the wax-dipping technique [57]; chlorophylls were additionally normalized to symbiont cell abundance [58].…”

Unique quantitative Symbiodiniaceae signature of coral colonies revealed through spatio-temporal survey in Moorea

“…The coral holobiont’s resilience to environmental stressors is in large part based on various genotypes and biological traits 19 . This includes the complex interaction and differential physiological responses of symbiotic Symbiodiniaceae populations that may be composed of one single genus or multiple genera and species 20,21 and the host 22–25 . A plethora of in situ and ex situ studies have reported highly dynamic coral-Symbiodiniaceae associations in response to environmental changes, contrasts or extremes 26–31 .…”

Nutrient Pollution and Predation Differentially Affect Innate Immune Pathways in the Coral Porites porites

“…While much remains to be understood, our knowledge of the diverse temporal and species-specific responses of phenoloxidase pathways in corals has grown in recent years to reflect the complexity of these pathways. Tyrosinase-type phenoloxidases have been shown to respond to heat stress (Mydlarz et al, 2009;Palmer et al, 2011a,b, Wall et al, 2018, disease (Mydlarz et al, 2008;Palmer et al, 2011a;Kelly et al, 2016), pathogen elicitors (Palmer et al, 2011b;Fuess et al, 2016), pathogens (van de Water, et al, 2018), tissue growth (D'Angelo et al, 2012), sedimentation (Sheridan et al, 2014), seasons (van de Water et al, 2015b(van de Water et al, , 2016, and damage (van de Water et al, 2015a,b). Tyrosinase-type POs have also been positively correlated with both disease and bleaching resistance variability between different coral species (Palmer et al, 2010(Palmer et al, , 2012a.…”