The effects of illumination, temperature and 6-benzylaminoprine on asymbiotic seed germination and protocorm development in vitro in the achlorophyllous orchid Gastrodia pubilabiata Sawa

Godo, Toshinari; Hashimoto, Toshimasa; Nakata, Masashi; Miyoshi, K.

doi:10.1007/s11627-020-10061-4

Cited by 6 publications

(3 citation statements)

References 23 publications

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“…Aseptic propagation via an embryogenic callus was demonstrated in Gastrodia elata , and the regenerated tubers continued to grow after inoculation of Armillaria isolates (Yeh et al 2017 ). Seeds of Gastrodia pubilabiata successfully germinated without symbionts, and their subsequent development was controlled by illumination (Godo et al 2020 ).…”

Section: Culturing Sap-mhpsmentioning

confidence: 99%

Evolutionary histories and mycorrhizal associations of mycoheterotrophic plants dependent on saprotrophic fungi

2021

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Mycoheterotrophic plants (MHPs) are leafless, achlorophyllous, and completely dependent on mycorrhizal fungi for their carbon supply. Mycorrhizal symbiosis is a mutualistic association with fungi that is undertaken by the majority of land plants, but mycoheterotrophy represents a breakdown of this mutualism in that plants parasitize fungi. Most MHPs are associated with fungi that are mycorrhizal with autotrophic plants, such as arbuscular mycorrhizal (AM) or ectomycorrhizal (ECM) fungi. Although these MHPs gain carbon via the common mycorrhizal network that links the surrounding autotrophic plants, some mycoheterotrophic lineages are associated with saprotrophic (SAP) fungi, which are free-living and decompose leaf litter and wood materials. Such MHPs are dependent on the forest carbon cycle, which involves the decomposition of wood debris and leaf litter, and have a unique biology and evolutionary history. MHPs associated with SAP fungi (SAP-MHPs) have to date been found only in the Orchidaceae and likely evolved independently at least nine times within that family. Phylogenetically divergent SAP Basidiomycota, mostly Agaricales but also Hymenochaetales, Polyporales, and others, are involved in mycoheterotrophy. The fungal specificity of SAP-MHPs varies from a highly specific association with a single fungal species to a broad range of interactions with multiple fungal orders. Establishment of symbiotic culture systems is indispensable for understanding the mechanisms underlying plant–fungus interactions and the conservation of MHPs. Symbiotic culture systems have been established for many SAP-MHP species as a pure culture of free-living SAP fungi is easier than that of biotrophic AM or ECM fungi. Culturable SAP-MHPs are useful research materials and will contribute to the advancement of plant science.

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Section: Culturing Sap-mhpsmentioning

confidence: 99%

Evolutionary histories and mycorrhizal associations of mycoheterotrophic plants dependent on saprotrophic fungi

2021

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“…Terrestrial orchids are quite different than epiphytic orchids in terms of asymbiotic seed germination protocol (De Pauw et al, 1995). There are numerous previous studies about asymbiotic germination using different media formulations on several terrestrial orchids genera such as Cypripedium (Chu and Mudge, 1996;De Pauw et al, 1996;Szendrak, 1997;Yan et al, 2006;Bae and Choi, 2008;Klavina et al, 2009;Huh et al, 2016;Huh et al, 2019), Dactylorhiza (Laurent et al, 2014;Gümüş et al, 2017), Serapias (Gümüş and Ellialtioglu, 2012;Bektas and Sokmen, 2016;Calevo et al, 2017;Acemi and Ozen, 2019), Cephalanthera (Szendrak, 1997;Hemrova et al, 2019), Paphiopedilum (Lee, 2007;Zeng et al, 2012), Chloraea (Pereira et al, 2017;Quiroz et al, 2017), Bletia (Dutra et al, 2008), Geodorum (Bhadra and Hossain, 2003), Habenaria (Stewart and Kane, 2006), Peristylus (Thakur and Dongarwar, 2017), Anacamptis (Magrini et al, 2019), Bipinnula (Pereira et al, 2015), Pecteilis (Kim et al, 2019), Epipactis (Hemrova et al, 2019), Himantoglossum (Szendrak, 1997;Dulic et al, 2019), Spathoglottis (Barrientos and Fang, 2019), Anoectochilus and Haemaria (Chou and Chang, 2004), Calopogon and Socoila (Kauth, 2005), Gastrodia (Godo et al, 2020), Calanthe (Bae and Kim, 2015), Spiranthes (Dulic et al, 2019), Ophrys, Barlia, and Platanthera (Szendrak, 1997;…”

Section: Introductionmentioning

confidence: 99%

Asymbiotic Germination and Seedling Development of Terrestrial Orchid Bletilla striata Using in vitro and ex vitro Cultures

Kahraman

Cullum²

2021

Horticultural Studies

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Procedures for asymbiotic germination and seedling development under in vitro and ex vitro conditions were investigated for Bletilla striata. Five different asymbiotic germination media (½ P6668 ¬Phytamax Orchid Maintenance Media, ½ P6668 ¬Phytamax Orchid Maintenance Media with coconut water, P723 PhytoTechnology Orchid Seed Sowing Media, P723 PhytoTechnology Orchid Seed Sowing Media with coconut water, and ½ MS media) for in vitro culture and sphagnum moss for ex vitro culture were examined for their effectiveness for 8 weeks on seed germination and seedling development of B. striata. Germination occurred in all media, however, the best germination rate was obtained in P723 medium with coconut water while the lowest frequency was obtained in sphagnum moss. Moreover, the highest leaf parameters of the seedlings of B. striata developed in ½ MS medium when the seedlings in sphagnum moss showed the lowest results. Furthermore, all germinated protocorms showed rhizoid formation in all media.

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“…Hence, there is a need to consider the type of explants used. Potential explants include the use of seeds (Utami & Hariyanto, 2019) as previously studied by Adhikari & Pant (2019), Dulić et al (2019), Godo et al (2020), and Manokari et al (2021). However, this study differs from previous studies, we provided C. rochussenii orchid seeds taken directly from the wildlife reserve area of Bukit Rimbang and Baling wildlife reserve areas.…”

Section: Introductionmentioning

confidence: 99%

Ex-situ conservation of the native orchid Coelogyne rochusseni de Vriese from the Bukit Rimbang and Bukit Baling Wildlife Reserve Areas

Heriansyah¹,

Marlina²

2021

bio

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The native orchid Coelogyne rochussenii de Vriese is under threat of extinction, so conservation measures need to be taken. In-situ conservation is constrained by time, resources and costs, and the appropriate conservation must be ex-situ. Therefore, This study aims to determine the most effective method for ex-situ conservation, especially basal medium and activated charcoal, in order to determine the fastest germination. The method used in this research was a factorial completely randomized design, consisting of four different basal medium treatments, Knudson C, Vacin and Went, Murashige and Skoog, and Hyponex + Vitamin medium. This treatment was combined with the application of activated charcoal at 4 different levels, with concentrations of 0.1,2.3 g/L-1, the parameters observed were germination day (day), germination percentage (%), and percentage of contamination (%). The results of this study concluded that the conservation of the Native orchid Coelogyne Rochussenii de Vriese using various growing mediums and activated charcoal had a significant effect on the growth of the embryo culture native orchid. Hyponex + Vitamin medium with 3 g/L-1 is able to accelerate germination days to 29.33 days, increase germination percentage to 92.06%, and reduce contamination to 0%.

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The effects of illumination, temperature and 6-benzylaminoprine on asymbiotic seed germination and protocorm development in vitro in the achlorophyllous orchid Gastrodia pubilabiata Sawa

Cited by 6 publications

References 23 publications

Evolutionary histories and mycorrhizal associations of mycoheterotrophic plants dependent on saprotrophic fungi

Evolutionary histories and mycorrhizal associations of mycoheterotrophic plants dependent on saprotrophic fungi

Asymbiotic Germination and Seedling Development of Terrestrial Orchid Bletilla striata Using in vitro and ex vitro Cultures

Ex-situ conservation of the native orchid Coelogyne rochusseni de Vriese from the Bukit Rimbang and Bukit Baling Wildlife Reserve Areas

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