The effects of inorganic additives to calcium phosphate on in vitro behavior of osteoblasts and osteoclasts

Yang, Liang; Pérez‐Amodio, Soledad; Groot, Florence Y.F. Barrère-de; Everts, Vincent; Blitterswijk, Clemens A. van; Habibović, Pamela

doi:10.1016/j.biomaterials.2010.01.002

Cited by 162 publications

(140 citation statements)

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“…The presence of zinc in b-TCP (up to 0.6 wt.%) has been shown to have a dose-dependent negative effect on the resorptive activity of primary mature rabbit osteoclasts, as indicated by an increase in the number of apopoptic osteoclasts and a decrease in actin ring formation [169,170]. Incorporation of zinc into thin apatite films also led to the decreased resorption by primary osteoclasts, whereas no effect was found on the initial attachment of cells [171].…”

Section: Zincmentioning

confidence: 96%

Bioinorganics and biomaterials: Bone repair

2011

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Section: Zincmentioning

confidence: 96%

Bioinorganics and biomaterials: Bone repair

2011

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“…11 In this manner, strontium ranelate is thought to prevent osteoporotic fractures via an increase in bone hardness due to the Sr 21 ionic substitution. 40,41 To further develop strontium-based treatments, the exact mechanisms of strontium's actions are yet to be fully elucidated.…”

mentioning

confidence: 99%

Human mesenchymal stromal cells response to biomimetic octacalcium phosphate containing strontium

Birgani

Malhotra

Blitterswijk

et al. 2016

J. Biomed. Mater. Res.

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The incorporation of bioinorganics into synthetic biomaterials is a promising approach to improve the biological performance of bone graft substitutes, while still retaining their synthetic nature. Among these bioinorganics, strontium ions (Sr(2+) ) have reported enhanced bone formation, and a reduced risk of bone fractures. While previous results have been encouraging, more detailed studies are needed to further develop specific applications. This study demonstrates the effects of Sr(2+) on the osteogenic differentiation of human mesenchymal stromal cells (hMSCs) when introduced as either a dissolved salt, or incorporated into biomimetic calcium phosphate (CaP) coatings. Upon attachment, hMSCs seeded in the presence of higher Sr(2+) concentrations presented with a more elongated shape as compared to the controls without Sr(2+) . Both Sr(2+) as a dissolved salt in the medium, or incorporated into CaP coatings, positively influenced hMSC alkaline phosphatase (ALP) activity in a dose-dependent manner. At the mRNA level, the expression of osteogenic markers ALP, bone sialoprotein, bone morphogenetic protein 2, osteopontin, and osteoclacin were increased in the presence of Sr(2+) , independent of the delivery method. Overall, this study demonstrates the positive effects of strontium on the osteogenic differentiation of human MSCs, and supports the use of strontium-incorporated CaPs for bone regeneration applications. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 1946-1960, 2016.

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“…The resorptive activity of primary rabbit osteoclasts cultured on calcium phosphate films containing additives significantly decreased and it was concentration-dependent as compared to the control, independent of the element incorporated. The elements in the tested concentrations showed no cytotoxic effect(L. Yang et al, 2010). In another study by Patntirapong et al, calcium phosphate film with Co 2+ incorporation increased both osteoclast differentiation and resorptive function (Patntirapong et al, 2009).…”

Section: Effects Of Biomimetic Apatite Coatings On In Vitro Behavior mentioning

confidence: 90%