The effects of local and intraperitoneal zinc treatments on maxillofacial fracture healing in rabbits

Azgın, İsa; Arbağ, Hamdi; Eryılmaz, Mehmet; Çelik, Zeliha Esin

doi:10.1016/j.jcms.2020.01.013

Cited by 4 publications

(6 citation statements)

References 29 publications

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“…This aligns with findings from Murray and Messer that zinc released from bone is re‐absorbed quickly 71 . In addition to the local injection used in this study, other delivery methods have been explored, including oral 55 and intraperitoneal supplementation 61 and local delivery to the site of injury using carriers to control zinc release 22 . Sustained release of zinc may produce better results in other contexts, such as segmental defects, spinal fusions, or complex fractures.…”

Section: Discussionsupporting

confidence: 83%

“…60 microplates coated with zinc to accelerate healing in a rabbit maxillofacial fracture model. 61 Another study found that zinc with IGF-1 supplementation improved osteoblastic proliferation in a hydroxyapatite implant study. 62 Zinc is the sixth most abundant metal in the human body.…”

Section: Discussionmentioning

confidence: 99%

“…Callus Zn levels were significantly greater than contralateral diaphysis values at 1 (p = 0.008) and 3 dpf (p = 0.002) as indicated (*). delivery methods have been explored, including oral 55 and intraperitoneal supplementation 61 and local delivery to the site of injury using carriers to control zinc release. 22 Sustained release of zinc may produce better results in other contexts, such as segmental defects, spinal fusions, or complex fractures.…”

Section: Discussionmentioning

confidence: 99%

“…In this same model, use of zinc oxide nanoparticles improved radiographic scoring 60 . Azgin et al used titanium microplates coated with zinc to accelerate healing in a rabbit maxillofacial fracture model 61 . Another study found that zinc with IGF‐1 supplementation improved osteoblastic proliferation in a hydroxyapatite implant study 62 …”

Section: Discussionmentioning

confidence: 99%

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Local zinc treatment enhances fracture callus properties in diabetic rats

Innella

Levidy

Kadkoy

et al. 2022

Journal Orthopaedic Research

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The effects of locally applied zinc chloride (ZnCl2) on early and late‐stage parameters of fracture healing were evaluated in a diabetic rat model. Type 1 Diabetes has been shown to negatively impact mechanical parameters of bone as well as biologic markers associated with bone healing. Zinc treatments have been shown to reverse those outcomes in tests of nondiabetic and diabetic animals. This study is the first to assess the efficacy of a noncarrier mediated ZnCl2 on bony healing in diabetic animals. This is a promising basic science approach which may lead to benefits for diabetic patients in the future. Treatment and healing were assessed through quantification of callus zinc, radiographic scoring, microcomputed tomography (µCT), histomorphometry, and mechanical testing. Local ZnCl2 treatment increased callus zinc levels at 1 and 3 days after fracture (p ≤ 0.025). Femur fractures treated with ZnCl2 showed increased mechanical properties after 4 and 6 weeks of healing. Histomorphometry of the ZnCl2‐treated fractures found increased callus cartilage area at Day 7 (p = 0.033) and increased callus bone area at Day 10 (p = 0.038). In contrast, callus cartilage area was decreased (p < 0.01) after 14 days in the ZnCl2‐treated rats. µCT analysis showed increased bone volume in the fracture callus of ZnCl2‐treated rats at 6 weeks (p = 0.0012) with an associated increase in the proportion of µCT voxel axial projections (Z‐rays) spanning the fracture site. The results suggest that local ZnCl2 administration improves callus chondrogenesis leading to greater callus bone formation and improved fracture healing in diabetic rats.

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Section: Discussionsupporting

confidence: 83%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Local zinc treatment enhances fracture callus properties in diabetic rats

Innella

Levidy

Kadkoy

et al. 2022

Journal Orthopaedic Research

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“…Its use has also expanded over the years for several dermatological conditions, including infections inflammatory dermatoses, pigmentary disorders and neoplasia. Therapeutically, Zn 2+ can be used both topically and in systemic form; however, systemic Zn 2+ therapy needs further experimental and clinical evidences [ 28 , 29 ]. Zn 2+ plays an important physiological function throughout the stages of wound healing, associated with inflammation and immune response [ 4 , 30 , 31 ].…”

Section: Introductionmentioning

confidence: 99%

Zinc Chloride: Time-Dependent Cytotoxicity, Proliferation and Promotion of Glycoprotein Synthesis and Antioxidant Gene Expression in Human Keratinocytes

Salesa

Serra

Serrano‐Aroca

2021

Biology

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The use of ionic metals such as zinc (Zn2+) is providing promising results in regenerative medicine. In this study, human keratinocytes (HaCaT cells) were treated with different concentrations of zinc chloride (ZnCl2), ranging from 1 to 800 µg/mL, for 3, 12 and 24 h. The results showed a time–concentration dependence with three non-cytotoxic concentrations (10, 5 and 1 µg/mL) and a median effective concentration value of 13.5 µg/mL at a cell exposure to ZnCl2 of 24 h. However, the zinc treatment with 5 or 1 µg/mL had no effect on cell proliferation in HaCaT cells in relation to the control sample at 72 h. The effects of the Zn2+ treatment on the expression of several genes related to glycoprotein synthesis, oxidative stress, proliferation and differentiation were assessed at the two lowest non-cytotoxic concentrations after 24 h of treatment. Out of 13 analyzed genes (superoxide dismutase 1 (SOD1), catalase (CAT), matrix metallopeptidase 1 (MMP1), transforming growth factor beta 1 (TGFB1), glutathione peroxidase 1 (GPX1), fibronectin 1 (FN1), hyaluronan synthase 2 (HAS2), laminin subunit beta 1 (LAMB1), lumican (LUM), cadherin 1 (CDH1), collagen type IV alpha (COL4A1), fibrillin (FBN) and versican (VCAN)), Zn2+ was able to upregulate SOD1, CAT, TGFB1, GPX1, LUM, CDH1, FBN and VCAN, with relative expression levels of at least 1.9-fold with respect to controls. We found that ZnCl2 promoted glycoprotein synthesis and antioxidant gene expression, thus confirming its great potential in biomedicine.

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