The effects of oral ketoconazole and griseofulvin on the fertility of male rabbits

Abstract

Objectives: To investigate possible side effects of ketoconazole and griseofulvin on fertility of clinically healthy male rabbits. Design: Randomized controlled experimental study. Animals: Thirty mature male rabbits. Procedures: Rabbits were randomly allocated into three equal sized groups (10 animals each). The first group served as a control group (received no treatment), the second group received griseofulvin (25 mg/kg B.W) orally for 30 days, and the third group received ketoconazole (50 mg/kg B.W) orally for 30 days. Semen samples were collected after 1 day, 1 week and 2 weeks post-administration to determine sperm motility, % of live and dead sperms, total sperm abnormalities and sperm cell concentration. Two weeks post-administration, testes and epididymides were removed for histopathological examination. Results: Both drugs produced a significant reduction in the serum testosterone level, sperm cell concentration, percent of live sperms and percent of sperm motility. Conclusion and clinical relevance: Ketoconazole and griseofulvin have a negative impact on fertility of male rabbits, and the effect is more pronounced with ketoconazole.

Total Aseptization of Boar Semen, to Increase the Biosecurity of Reproduction in Swine

“…There is little information in the literature about fungal contamination of boar semen and its persistence during storage [ 16 , 17 ]. The usual conditions of storage before AI (liquid state at 17 °C for several days) represent excellent conditions for the development of fungi, and the extenders do not contain antifungal substances.…”

Ameliorating Effect of Linseed Oil Against Fluconazole Induced Adverse Effect on Male Fertility of Cocks

“…Also, Amer et al (2018) reported that testes of fluconazole medicated cocks showed vacuolated spermatocytes with more widening diameter of seminiferous tubule and loss of spermatocytes inside the dilated lumen of seminiferous tubules. Similarly, Mohamed et al (2020) stated that, the testes of ketoconazole treated animals showed marked interstitial fibrosis and edema with severe tubular degeneration and desquamation of germ cells in lumen and arrested spermatogenesis. Testes of linseed oil supplemented group for 2 weeks after fluconazole administration showed widening in luminal diameter of seminiferous tubules, besides, mildly vacuolated spermatocytes in testes of treated cocks.…”

“…Similar finding has reported that administration of fluconazole at both therapeutic and double therapeutic doses induced a significant decrease in serum testosterone level (Hammad et al, 2018). Moreover, Mohamed et al (2020) found that ketoconazole administration induced a prominent decrease in serum testosterone level in treated rabbits. The different small letters in the same column means that there were significant changes at P <0.05.…”

“…Similarly, Amer et al (2018) revealed that, there were a significant decrease in the sperm count in fluconazole medicated cocks. Moreover, Mohamed et al (2020) declared that ketoconazole administration induced a marked decrease in semen volume, sperms concentration, live sperms % and sperm motility % and increase the morphological abnormalities in sperms of treated rabbits.…”

Total Aseptization of Boar Semen, to Increase the Biosecurity of Reproduction in Swine

“…The literature in poor in information about fungal contamination of boar semen and its persistence during storage [11,31]. The usual conditions of storage before AI (liquid state at +17°C for several days) represent excellent conditions for the development of fungi, and the extenders do not contain antifungal substances.…”