The effects of protein kinase A catalytic subunit on sperm motility regulation in Pacific abaloneHaliotis discus hannai

Abstract

Sperm motility, the ability of sperm to move towards the egg, determines the fertilization rate, which is considered as an important indicator of the quality and fertilizing capacity of semen (Mizrahi & Breitbart, 2014;Turner, 2003). It has been documented that sperm motility is regulated by the signalling events that control sperm ion milieu, energy production and classical second messenger-dependent phosphorylation cascades (Xie, Eddy, & Conti, 2013). cAMP signalling pathway is the best characterized event, which plays a central role in the initiation and modulation of sperm motility from echinoderms to mammals (Nomura, Beltran, Darszon, & Vacquier, 2005).Protein kinase A (PKA) is one of the major downstream effectors of cAMP signals, which regulates sperm motility by phosphorylating serine/threonine residues on neighbouring proteins (Pereira, Sã, Barros, & Sousa, 2017). The inactive PKA is a tetrameric enzyme

Effects of Cryopreservation on Gene Expression and Post Thaw Sperm Quality of Pacific Abalone, Haliotis discus hannai

“…Cryopreserved sperm of Atlantic salmon also shows reduced PKA activity than fresh sperm (Lee-Estevez et al, 2019). Kong et al (2020) have reported that PKA-C can regulate sperm motility of Pacific abalone. Considering the above facts, reduced motility of post thaw sperm might be responsible for the lower mRNA expression of PKA-C gene found in the present study.…”

“…Pacific abalone, Haliotis discus hannai, is known as "soft gold" of the ocean due to its high commercial importance (Kong et al, 2020). It is a high-priced seafood item in South Korea, Taiwan, Japan, and China because it contains bioactive molecules with anticancer, antioxidant, anti-inflammatory, antimicrobial, and antithrombotic activities (Suleria et al, 2017;Sharker et al, 2020b).…”

“…CatSper channels are crucial for motility and fertility of sperm (Mannowetz et al, 2017). Protein kinase A (PKA-C) is a sperm motility regulating gene in Pacific abalone (Kong et al, 2020) and other aquatic fish (Zilli et al, 2017) and shellfish (Nomura et al, 2005;Vacquier et al, 2014). The freeze-thaw process of cryopreservation can reduce the motility and viability of post thaw sperm.…”

Molecular Cloning and Functional Characterization of Catalase in Stress Physiology, Innate Immunity, Testicular Development, Metamorphosis, and Cryopreserved Sperm of Pacific Abalone

“…Of various abalone species, Pacific abalone Haliotis discus hannai is the most demanded seafood in Korea, Taiwan, China, and Japan, because it contains bioactive molecules [40]. It is also known as the "emperor of shellfish" in Korea [41] and recognized worldwide as the "soft gold" of the Ocean [42,43]. Among abalone, to date, CAT has been reported in disk abalone, H. discus discus [3].…”

Antioxidant Activity and Oxidative Stress-Oriented Apoptosis Pathway in Saccharides Supplemented Cryopreserved Sperm of Pacific Abalone, Haliotis discus hannai

“…Pacific abalone, Haliotis discus hannai is high-demandable and high-priced seafood because it contains antioxidant- and anticancer-activated bioactive molecules [ 1 , 2 ]. In South Korea, it is known as the “emperor of shellfish” [ 3 ], and worldwide known as “soft gold” of the Ocean [ 4 ]. Commercial fertilization of Pacific abalone depends on broodstock conditioning through effective accumulating temperature (EAT).…”