The effects of various substances on adenosine triphosphatase activity in the erythrocyte membrane

Mircevová, L; Simonová, A

doi:10.1135/cccc19664145

Cited by 6 publications

(3 citation statements)

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“…After digitonin treatment only the Mg++ dependent ATPase [15] is stimulated, lysolecithin and saponin treat ment stimulates both ATPase components, the Mg++ dependent and Mg++-|-Na+-|-K+ dependent ATPase. The increase of ATPase activity due to the above-mentioned hemolysins may be due neither to changes in the protein nor to the free cholesterol level, as no relation to the ATPase activity was found.…”

Section: Discussionmentioning

confidence: 99%

“…As it was found in our previous paper [15] that after the treatment with a certain concentration of saponin or digitonin the ATPase activity of the ghosts increases and as a higher activity was established in sa ponin-treated ghosts in hereditary spherocytosis [14] i.e. in a disease where the erythrocytes have an altered globular shape, a certain connection between the shape of the ghosts and the ATPase activity was considered.…”

Section: Introductionmentioning

confidence: 99%

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The Influence of Hemolysins on the ATPase Activity and the Composition of the Erythrocyte Membrane

Mircevová¹,

Simonová²,

Michalec³

et al. 1968

Enzymol Biol Clin

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Summary. The influence of low concentrations of some hemolysins on the erythrocyte membrane was investigated. Lysolecithin stimulates Mg^++ as well as Mg^++ + Na^+ + K^+ dependent ATPase in ghosts. Lecithin slightly stimulates ATPase only in saponin-treated ghosts. After saponin and digitonin treatment in concentrations enhancing the ATPase activity less phospholipids were eluted from ghosts than after water; saponin does not significantly change the level of free cholesterol, digitonin decreases it, while that of cholesterol esters remains unaltered. The protein content of ghosts was determined after saponin, digitonin, lysolecithin and lecithin treatment. The significant change, namely decrease, was found after treatment with saponin in physiological saline and with digitonin both in distilled water and physiological saline; after lecithin treatment somewhat less protein was washed from ghosts' than after distilled water. The obtained results are discussed with respect to the shape of the erythrocytes and the ATPase activity.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The Influence of Hemolysins on the ATPase Activity and the Composition of the Erythrocyte Membrane

Mircevová¹,

Simonová²,

Michalec³

et al. 1968

Enzymol Biol Clin

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“…Its function and role in the membrane has not yet been clarified, nor was its relation to Mg2+, Ca2+-dependent ATPase. It is to be found there in a latent form [11] and can be activated, for instance, by a certain disruption of the membrane [4,5,12]. Wins and Schoffeniels [14] found out that erythrocyte Mg2+, Ca2+-dependent ATPase can be influenced by substances interfering with the activity of flavoenzymes and suggest that ATPase activity can be controlled by the redox state of an electron carrier associated to it.…”

Section: Introductionmentioning

confidence: 99%

The Effect of Bathophenanthroline and Hydroxylamine

Simonová¹,

Mircevová²

1974

Enzyme

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Bathophenanthroline has no effect on the activity of latent Mg^2+-dependent ATPase in human erythrocytes, but inhibits that of saponin-stimulated enzyme (K(i) = 1.5 x 10^-2 mol/l). Hydroxylamine takes a different action. Enzyme activity increases with increasing concentration of hydroxylamine, reaching its peak at approximately 10 mmol/l; a further increase in concentration results in gradual decrease of activity. Saponin-stimulated activity of Mg^2+-dependent ATPase is inhibited; a half maximum activity is reached at approx. 60 mmol/l hydroxylamine.

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The composition of red cell lipids and the activity of two enzymes of the erythrocyte membrane (ATPase and acetylcholinesterase) in primary refractory anemias

Palek¹,

Brabec²,

Vopatová³

et al. 1969

Clinica Chimica Acta

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The effects of various substances on adenosine triphosphatase activity in the erythrocyte membrane

Cited by 6 publications

References 0 publications

The Influence of Hemolysins on the ATPase Activity and the Composition of the Erythrocyte Membrane

The Influence of Hemolysins on the ATPase Activity and the Composition of the Erythrocyte Membrane

The Effect of Bathophenanthroline and Hydroxylamine

The composition of red cell lipids and the activity of two enzymes of the erythrocyte membrane (ATPase and acetylcholinesterase) in primary refractory anemias

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