The aim of the study was to compare sulfate fertilizers and mixtures of elemental sulfur (S0) and sulfate in terms of yield and nitrogen (N) and sulfur (S) status in perennial ryegrass. Mixtures of sulfate and S0 can reduce the consumption of sulfate alone. The plants were grown in soil cultures. The plants were supplemented with S0, K2SO4, MgSO4, and (NH4)SO4 or a mixture of these salts with So. Two sulfur doses were applied and the ryegrass was harvested three times. Fresh and dry weights of each swath, the N and S content, and their uptake were determined. The total fresh yield of sulfur-fertilized plants was 25 to 94% higher compared to unfertilized plants. The increases in dry matter were even more significant. Fertilizers, being a mixture of S0 and sulfate, showed the same efficiency as those containing sulfate alone. Sulfur fertilization resulted in a higher S content and its uptake, lowered N concentration in second and third swatch, and a decrease in total N uptake. In conclusion, to achieve high crop yields, soil sulfur deficiency should be corrected and fertilizers that are the mixture of elemental sulfur and sulfate are a beneficial and effective approach.