The Ehrlich Pathway for Fusel Alcohol Production: a Century of Research on
            <i>Saccharomyces cerevisiae</i>
            Metabolism

Hazelwood, L.A.; Daran, Jean‐Marc; Maris, Antonius J. A. van; Pronk, Jack T.; Dickinson, J. Richard

doi:10.1128/aem.00934-08

Cited by 338 publications

(481 citation statements)

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“…Inui et al [16] [12] also reconstructed the butanol biosynthetic pathway in E. coli. Unlike Hanai et al, the authors expressed the complete butanol biosynthetic pathway from C. acetobutylicum in a single vector.…”

Section: Heterologous Expression Of the Clostridialmentioning

confidence: 99%

“…Briefly, the amino acid biosynthetic pathway generates a number of keto acid intermediates. The yeast S. cerevisiae converts the keto acids in the leucine, valine, isoleucine, phenylalanine, tryptophan, and methionine pathways into "fusel" alcohols as a byproduct of fermentation [16].Yeast produces these alcohols using the "Ehrlich pathway,"…”

Section: Re-routing Of the Amino Acid Biosynthetic Pathway For Producmentioning

confidence: 99%

“…by sequential keto acid decarboxylation to the aldehyde followed by a reduction of the aldehyde to the alcohol [16]. Via the Ehrlich pathway it may be possible to overproduce: propanol and 2-methyl-1-butanol (2MB) from 2-ketobutyrate and 2-keto-3-methylvalerate (KMV), respectively, which are intermediates in the biosynthesis of isoleucine; isobutanol from 2-ketoisovalerate, an intermediate in biosynthesis of valine; 3-methyl-1-butanol (3MB) from 2-keto-4-methylpentanoate, an intermediate in the biosynthesis of leucine; 2-phenylethanol from phenylpyruvate, an intermediate in the biosynthesis of phenylalanine; and 1-butanol from 2-ketovalerate, a precursor in the norvaline biosynthetic pathway.…”

Section: Re-routing Of the Amino Acid Biosynthetic Pathway For Producmentioning

confidence: 99%

“…In eukaryotes, the four enzymes necessary for fatty acid elongation are contained in a multidomain protein (type 1 fatty acid synthase), while in E. coli each enzymatic activity in the elongation step is performed by a monofunctional enzyme (type 2 fatty acid synthase). Once the fatty acid has reached a certain carbon length (e.g., C 16 ), in eukaryotes a thioesterase hydrolyzes the fatty acid-ACP to generate free fatty acid. In prokaryotes, the fatty acid-ACP is directly transferred to glycerol-3-phosphate without releasing a free fatty acid.…”

Section: Metabolic Engineering Of the Fatty Acid Biosynthetic Pathwaymentioning

confidence: 99%

“…Finally, jet fuel, the fuel for gas turbines, is a mixture of C 8 -C 16 hydrocarbons. Jet fuel has a maximum of 25% aromatic compounds in the fuel mixture.…”

Section: Introductionmentioning

confidence: 99%

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Advanced biofuel production in microbes

Peralta‐Yahya

Keasling

2010

Biotechnology Journal

349

226

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The cost-effective production of biofuels from renewable materials will begin to address energy security and climate change concerns. Ethanol, naturally produced by microorganisms, is currently the major biofuel in the transportation sector. However, its low energy content and incompatibility with existing fuel distribution and storage infrastructure limits its economic use in the future. Advanced biofuels, such as long chain alcohols and isoprenoid-and fatty acid-based biofuels, have physical properties that more closely resemble petroleum-derived fuels, and as such are an attractive alternative for the future supplementation or replacement of petroleum-derived fuels. Here, we review recent developments in the engineering of metabolic pathways for the production of known and potential advanced biofuels by microorganisms. We concentrate on the metabolic engineering of genetically tractable organisms such as Escherichia coli and Saccharomyces cerevisiae for the production of these advanced biofuels.

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Section: Heterologous Expression Of the Clostridialmentioning

confidence: 99%

Section: Re-routing Of the Amino Acid Biosynthetic Pathway For Producmentioning

confidence: 99%

Section: Re-routing Of the Amino Acid Biosynthetic Pathway For Producmentioning

confidence: 99%

Section: Metabolic Engineering Of the Fatty Acid Biosynthetic Pathwaymentioning

confidence: 99%

“…Finally, jet fuel, the fuel for gas turbines, is a mixture of C 8 -C 16 hydrocarbons. Jet fuel has a maximum of 25% aromatic compounds in the fuel mixture.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Advanced biofuel production in microbes

Peralta‐Yahya

Keasling

2010

Biotechnology Journal

349

226

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Recent advances in the microbial production of C4 alcohols by metabolically engineered microorganisms

Yoo

Sohn

Son

et al. 2021

Biotechnology Journal

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Background:The heavy global dependence on petroleum-based industries has led to serious environmental problems, including climate change and global warming. As a result, there have been calls for a paradigm shift towards the use of biorefineries, which employ natural and engineered microorganisms that can utilize various carbon sources from renewable resources as host strains for the carbon-neutral production of target products.Purpose and Scope: C4 alcohols are versatile chemicals that can be used directly as biofuels and bulk chemicals and in the production of value-added materials such as plastics, cosmetics, and pharmaceuticals. C4 alcohols can be effectively produced by microorganisms using DCEO biotechnology (tools to design, construct, evaluate, and optimize) and metabolic engineering strategies. Summary of New Synthesis and Conclusions:In this review, we summarize the production strategies and various synthetic tools available for the production of C4 alcohols and discuss the potential development of microbial cell factories, including the optimization of fermentation processes, that offer cost competitiveness and potential industrial commercialization.

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Metabolic engineering of Saccharomyces cerevisiae for the production of 2‐phenylethanol via Ehrlich pathway

Kim

Cho

Hahn³

2013

Biotech & Bioengineering

129

109

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2-Phenylethanol (2-PE), a fragrance compound with a rose-like odor, is widely used in perfumery and cosmetics. Here, we report the first metabolic engineering approach for 2-PE production in Saccharomyces cerevisiae. 2-PE can be produced from the catabolism of L-phenylalanine via Ehrlich pathway, consisting of transamination to phenylpyruvate by Aro9, decarboxylation to phenylacetaldehyde by Aro10, and reduction to 2-PE by alcohol dehydrogenases. We demonstrated that Ald3 is mainly responsible for phenylacetaldehyde oxidation, competing with 2-PE production. ALD3 deletion strain overexpressing ARO9 and ARO10 both by episomal overexpression and by induction of the endogenous genes through overexpression of Aro80 transcription factor, produced 4.8 g/L 2-PE in a medium containing 10 g/L L-phenylalanine as a sole nitrogen source. Considering the cytotoxicity of 2-PE, this production titer is almost the upper limit that can be reached in batch cultures, suggesting the great potential of this yeast strain for 2-PE production. 2-PE production was further increased by applying two-phase fermentation method with polypropylene glycol 1200 as an extractant, reaching 6.1 g/L 2-PE in organic phase with the molar yield of 82.5%, which is about ninefold increase compared with wild type.

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The Ehrlich Pathway for Fusel Alcohol Production: a Century of Research on Saccharomyces cerevisiae Metabolism

Cited by 338 publications

References 0 publications

Advanced biofuel production in microbes

Advanced biofuel production in microbes

Recent advances in the microbial production of C4 alcohols by metabolically engineered microorganisms

Metabolic engineering of Saccharomyces cerevisiae for the production of 2‐phenylethanol via Ehrlich pathway

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