The Electrochemical Behavior of Hemoglobin on SWNTs/DDAB Film Modified Glassy Carbon Electrode

The presence of toxic acrylamide in a wide range of food products such as potato crisps, French fries or bread has been confirmed by Swedish scientists from Stockholm University. The neurotoxicity, possible carcinogenicity of this compound and its metabolites compels us to control them by quantitative and qualitative assays. Acrylamide forms adduct with hemoglobin (Hb) as a result of the reaction the -NH2 group of the N-terminal valine with acrylamide. In this work we present the use of glassy carbon electrodes coated with single-walled carbon nanotubes (SWCNTs) and Hb for voltammetric detection of acrylamide in water solutions. The electrodes presented a very low detection limit (1.0×10-9 M). The validation made in the matrix obtained by water extraction of potato crisps showed that the electrodes presented are suitable for the direct determination of acrylamide in food samples.

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“…Many procedures involve gold nanoparticles [22-28], single- and multi-wall carbon nanotubes [21, 29-32], whisker-like carbon composites [33]. …”

Section: Introductionmentioning

confidence: 99%

A Voltammetric Biosensor Based on Glassy Carbon Electrodes Modified with Single-Walled Carbon Nanotubes/Hemoglobin for Detection of Acrylamide in Water Extracts from Potato Crisps

Krajewska

Radecki

Radecka

2008

Sensors

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“…Carbon nanotubes have also been applied for this purpose because of their remarkable electrocatalytic properties. [22] As an excellent electron acceptor, C 60 could be dispersed in matrices, such as carbon nanotubes, to facilitate electron transfer. This indeed has been achieved by embedding fullerenes in didodecyldimethylammonium bromide (DDAB) membranes.…”

mentioning

confidence: 99%

Significantly Accelerated Direct Electron‐Transfer Kinetics of Hemoglobin in a C₆₀‐MWCNT Nanocomposite Film

Zhang

Fan

Yang

2006

Chemistry A European J

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The direct electrochemistry of hemoglobin (Hb) was studied in a novel all-carbon nanocomposite film of C(60)-MWCNT (MWCNT=multiple-walled carbon nanotube) and compared with that in bare MWCNT film. The heterogeneous electron-transfer rate constant k(s) of Hb/C(60)-MWCNT was determined to be 0.39 s(-1), which is more than one order of magnitude greater than that of Hb/MWCNT (0.03 s(-1)). The significantly accelerated electron-transfer kinetics are attributed to the mediator role played by C(60), which is finely dispersed on the MWCNT surfaces. The excellent stability of Hb/C(60)-MWCNT was established and its potential application towards the electrocatalytic reduction of O(2) was demonstrated.

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“…In order to improve the slow rates of electron transfer between hemoglobin and solid electrodes, functionalized electrodes using mediators, promoters or nanomaterials have been reported by several groups [11][12][13][14]23]. Films cast from didodecyldimethylammonium bromide (DDAB) microemulsions containing myoglobin onto pyrolytic graphite electrodes gave good electron transfer properties [24].…”

Section: Enhanced Electron Transfer For Hemoglobin In Hemoglobin-surfmentioning

confidence: 99%

“…The direct electron transfer was greatly enhanced for hemoglobin as well as myoglobin in stable lamellar liquid crystal surfactant films [12][13]. Most recently, the electrochemical studies of hemoglobin have also involved primarily heterogeneous electron transfer based on the nano-structural materials [14][15][16][17]. However, it still remains a big challenge to obtain direct and rapid electron transfer of hemoglobin at a plain solid electrode.…”

Section: Introductionmentioning

confidence: 99%

Comparison of Kinetics of Hemoglobin Electron Transfer in Solution and Immobilized on Electrode Surface

Li¹,

Liu²,

Prabhulkar³

2009

Am. J. Biomed. Sci.

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The electron transfer properties of horse heart hemoglobin are compared using a hydrophilic indium tin oxide electrode and a dilaurydimethylammonium bromide (DDAB) polymer modified PG electrodes. A clear, stable and quasi-reversible redox curve of hemoglobin (Hb) was obtained in the absence of electron transfer mediators or promoters. The formal redox potential of -0.17 vs. Ag/AgCl of Hb was determined. The effects of the sixth axial binding ligands including cyanoand azido binding ligands on the redox properties of Hb were investigated. There are no significant impacts of these ligands on the redox properties of Hb, whereas big effects of the binding ligand on the electrochemical properties of myoglobin were reported. The cyclic voltammetric behavior of hemoglobin was also studied in a DDAB film coated on a pyrolytic graphite electrode. Surface immobilized Hb in DDAB film gave direct, quasi-reversible, nondiffusion-kinetic-controlled electron transfer on the electrode surface. The formal redox potential for Hb in the DDAB film negatively shifted about 140 mV as compared to the diffusion-controlled redox reaction of Hb.

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The Electrochemical Behavior of Hemoglobin on SWNTs/DDAB Film Modified Glassy Carbon Electrode

Cited by 28 publications

References 36 publications

A Voltammetric Biosensor Based on Glassy Carbon Electrodes Modified with Single-Walled Carbon Nanotubes/Hemoglobin for Detection of Acrylamide in Water Extracts from Potato Crisps

A Voltammetric Biosensor Based on Glassy Carbon Electrodes Modified with Single-Walled Carbon Nanotubes/Hemoglobin for Detection of Acrylamide in Water Extracts from Potato Crisps

Significantly Accelerated Direct Electron‐Transfer Kinetics of Hemoglobin in a C₆₀‐MWCNT Nanocomposite Film

Comparison of Kinetics of Hemoglobin Electron Transfer in Solution and Immobilized on Electrode Surface

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The Electrochemical Behavior of Hemoglobin on SWNTs/DDAB Film Modified Glassy Carbon Electrode

Cited by 28 publications

References 36 publications

A Voltammetric Biosensor Based on Glassy Carbon Electrodes Modified with Single-Walled Carbon Nanotubes/Hemoglobin for Detection of Acrylamide in Water Extracts from Potato Crisps

A Voltammetric Biosensor Based on Glassy Carbon Electrodes Modified with Single-Walled Carbon Nanotubes/Hemoglobin for Detection of Acrylamide in Water Extracts from Potato Crisps

Significantly Accelerated Direct Electron‐Transfer Kinetics of Hemoglobin in a C60‐MWCNT Nanocomposite Film

Comparison of Kinetics of Hemoglobin Electron Transfer in Solution and Immobilized on Electrode Surface

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Significantly Accelerated Direct Electron‐Transfer Kinetics of Hemoglobin in a C₆₀‐MWCNT Nanocomposite Film