The Electron Transfer System of Skunk Cabbage Mitochondria.

Chance, Britton; Hackett, David P.

doi:10.1104/pp.34.1.33

Cited by 56 publications

(34 citation statements)

References 10 publications

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“…fig 2). It t;herefore seems likely that the extra reduction indtuced by dithionite affects some other component than the different b cytochromes (7,14). In that case, the vailues reported in tables I and HI for the cytochromes b should be lowered by a factor of aboult one-third.…”

Section: Resultsmentioning

confidence: 99%

The Respiratory Chain Components of Higher Plant Mitochondria

1968

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Abstract. Tightly coupled mitochondria have been prepared from a variety of plant sources: white potato (Solanum tuberosum), Jerusalem artichoke (Heliantus tuberosus), cauliflower buds (Brassica oleracea), and mung bean hypocotyls (Phaseolus aureus). Mitochondria with no appreciable coupling were also prepared from skunk cabbage spadices (Symplocarpus foetidus).Room temperature difference spectra show that these mitochondria are very similar in the qualitative and quantitative composition of their electron carriers. The For all kinds of mitochondria, the rates of oxidation of succinate are similar as well as the turnover of cytochrome oxidase sec'1), regardless of the metabolic activities of the tissues. The number of mitochondria per cell appears to be the controlling factor of the intensity of tissue respiration.Higher plant tilssues are characterized by a grea'ter number of components in the mitochondrial respiratory chain than i,s the case wi'th animal tissues (6,7,37,44 (20). However, necessary adjustments had to be made to take into account the fact that the absorption peaks of plant electron carriers are not always the same as those of simitlar carriers of animail mi;tochondria.The identifi'cation of the various cytochromes present in plant mitochondria was best performed at liquid nitrogen temperature (77°K). The oxidized and reduced samples were placed in a 3 mm light path cuvette and cooled in a Dewar flask filled with liquid nitrogen during the scanning of the spectrum (6, 21). Low temperature brings a higher resolution as wdlHl as an enhancement of the absorption peak. By scanning the same sample, first at room temperature and then at low temperature, it is possible to measure the magnitude of the low temperature en'hancement and, by using the same extinction coefficients as before (20) Figure 1 shows a series of room tempera,ture difference spectra obtained with Jerusa,lem artichoke mitoohondria. The tipper spectrum shows a clear identification of the cytochromes after reduotion by dithionite. The a!bsorption peak of cytochrome oxidase (cytochrome a + cytoohrome a3) is located at 602 mju, a few mjm closer to the blue region of the spectrum than cytoc-hrome oxidase from animal mitochondria whose a-band is generally found at 605 mu (52). T.he a-band

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Section: Resultsmentioning

confidence: 99%

The Respiratory Chain Components of Higher Plant Mitochondria

1968

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“…Interest in respiration has centred around the presence, characteristics and role of inhibitor stimulated oxygen uptake a property which is widespread among the algae. Despite numerous studies [2][3][4][5][6][7][8] the mechanism of inhibitor stimulated respiration is unknown.…”

Section: Introductionmentioning

confidence: 99%

A computer analysis of cyanide stimulated oxygen uptake in Chlorella protothecoides

Clore

Chance

1977

FEBS Letters

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“…Many of the electron carriers and catalytic proteins for electron transport from reduced diphosphopyridine nucleotide (DPNH), reduced triphosphopyridine nucleotide (TPNH) or succinate to oxygen have been demonstrated in particulate preparations from potato tubers (7), wheat roots (14), pea seedlings (6,19), avocado fruits (3), silver beet petiole (15), lupine seedlings (12), Aroid spadix (4,5,8,17), and tobacco roots (18).…”

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confidence: 99%