The emerging contaminant 3,3′-dichlorobiphenyl (PCB-11) impedes Ahr activation and Cyp1a activity to modify embryotoxicity of Ahr ligands in the zebrafish embryo model (Danio rerio)

Roy, Monika A.; Sant, Karilyn E.; Venezia, Olivia; Shipman, Alix B.; McCormick, Stephen D.; Saktrakulkla, Panithi; Hornbuckle, Keri C.; Timme‐Laragy, Alicia R.

doi:10.1016/j.envpol.2019.113027

Cited by 22 publications

(9 citation statements)

References 62 publications

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“…CYP1A is commonly associated with the biotransformation of environmental contaminants, especially the DLCs (Cardoso et al, 2019;Licata et al, 2019). Many DLCs including pesticides belong to the substrates of CYP1A enzyme biotransformation, which are usually converted to more hydrophilic metabolites for excretion (Roy et al, 2019). Besides, CYP1A induction can also produce toxic effects by promoting the production of active metabolites (Voelker et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

Mepanipyrim and cyprodinil induce divergent temporal patterns of AhR-mediated responses in zebrafish (Danio rerio) embryos and larvae

Shen

Tang

Zhu

et al. 2021

Preprint

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Mepanipyrim and cyprodinil are widely used to control and/or prevent fungal diseases in fruit culture. They are widely detected in aquatic environment and numerous food commodities including fruit and fruit products. Different from TCDD, mepanipyrim and cyprodinil are more easily degraded and metabolized in the environment. However, the in vivo analysis of their metabolic dynamics is unclear and need to be further confirmed. In this study, zebrafish embryos were constantly exposed to 100 µg/L mepanipyrim or cyprodinil for 7 days. The temporal pattern of CYP1A and AhR2 expression and EROD enzyme activity at different time frames during embryonic and larval development of zebrafish were investigated. Our results showed that mepanipyrim and cyprodinil tend to accumulate in zebrafish during early embryonic developmental stages. Meanwhile, mepanipyrim and cyprodinil exposure could increase the expression level of cyp1a and ahr2 genes and EROD activity by a dynamic pattern in different developmental stages of zebrafish. Besides, their metabolites, which may accumulate in the zebrafish larvae, have strong AhR agonistic activity and showed strong AhR binding ability. Importantly, the risk of exposure to pesticides in embryo stage is huge, and should be paid more attention.

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Section: Discussionmentioning

confidence: 99%

Mepanipyrim and cyprodinil induce divergent temporal patterns of AhR-mediated responses in zebrafish (Danio rerio) embryos and larvae

Shen

Tang

Zhu

et al. 2021

Preprint

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“…The seemingly ubiquitous presence of PCB11, a byproduct in the production of yellow paint pigments, occurs in terrestrial and aquatic ecosystems including the HR and its presence has raised concerns for its bioaccumulative properties and toxicity (Rodenburg et al 2015). Although PCB11 toxicity is only beginning to be addressed, initial studies in rodent models suggest that it is neurotoxic (Sethi et al 2018) and studies in zebra sh found it to be a partial agonist/antagonist of the AHR pathway that may modify the toxicity of co-occurring coplanar congeners (Roy et al 2019). PCB11 was detectable in livers of both HR sturgeons and it was ve-fold higher in shortnose sturgeon (mean 398 pg/g ww) than Atlantic sturgeon (mean 59.9 pg/g ww).…”

Section: Inadvertent Pcbs In Hudson River Sturgeonsmentioning

confidence: 99%

“…In addition, recent studies indicate the presence in some environmental samples of several PCB congeners that are not part of legacy Aroclor mixtures but are byproducts during manufacturing of other products such as certain paint pigments and are called 'inadvertent PCBs' (Vorkamp 2016). The toxicities of inadvertent PCBs are only beginning to be evaluated (Roy et al 2019).…”

Section: Introductionmentioning

confidence: 99%

Hepatic Burdens of PCB and PCDD/F Congeners in Federally Endangered Shortnose Sturgeon and Atlantic Sturgeon from the Hudson River, New York, USA: Burden Patterns and Potential Consequences in Offspring

Wirgin¹,

Chambers

2021

Preprint

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Sturgeon populations worldwide are threatened with extirpation but little is known about their tendency to bioaccumulate contaminants and their sensitivities to environmental burdens of these contaminants. Shortnose sturgeon and Atlantic sturgeon, two species that are federally endangered in the U.S., co-occur in the Hudson River (HR) where high sediment levels of PCBs and PCDD/Fs occur. Previous controlled laboratory studies showed that young life-stages of both species are sensitive to toxicities at low levels of TCDD and PCB126 exposure. The objective here was to measure congener-specific hepatic levels of PCBs and PCDD/Fs in HR specimens in order to determine if in situ bioaccumulation of these compounds was sufficiently high to cause the early life-stage toxicities previously observed. Estimates of hepatic burdens of PCBs and PCDD/Fs were obtained from a small number of specimens of each species collected between 2014 and 2016 and specimens of shortnose sturgeon collected over 30 yr earlier and archived in a museum collection. Several significant patterns emerged. Hepatic levels of legacy PCBs and PCDDs were low in specimens of both species, but typically higher in shortnose than Atlantic sturgeon, a pattern consistent with their habitat use in the HR. Hepatic burdens from archived specimens of shortnose sturgeon tended to be higher than more recently collected ones despite expected reduction in their burdens due to preservation methods. Several inadvertent PCBs congeners were detected, including PCB11, but their possible toxicity to natural populations remains to be determined in future experiments. Levels of select PCDFs congeners, 2,3,7,8-TCDF and 2,3,4,7,8 PeCDF, were elevated in some shortnose sturgeon individuals from the HR. Using Relative Potency (ReP) factors derived from white sturgeon, the observed levels of some hepatic PCDFs in HR shortnose sturgeon may have been sufficiently high to impair recruitment of young life-stages in this ecosystem.

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“…Cytochrome P450 (CYP) enzymes were reported as the biomarkers to estimate the influences of persistent organic pollutants (POPs) on various aquatic organisms [ 17 , 18 , 19 ]. PAHs, which are known agonists for controlling aromatic hydrocarbon receptors [ 20 , 21 ], could induce the expressions of Phase I and Phase II metabolism enzymes including cytochrome P4501A (CYP1A) and glutathione S-transferase (GST).…”

Section: Introductionmentioning

confidence: 99%

Long-Term Exposure to Phenanthrene Induced Gene Expressions and Enzyme Activities of Cyprinus carpio below the Safe Concentration

Kang

Zhao

et al. 2022

IJERPH

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Phenanthrene (PHE) is a typical compound biomagnified in the food chain which endangers human health and generally accumulates from marine life. It has been listed as one of the 16 priority PAHs evaluated in toxicology. In order to evaluate the changes of CYP1A GST mRNA expression and EROD GST enzyme activity in carp exposed to lower than safe concentrations of PHE. Long-term exposure of carp to PHE at lower than safe concentrations for up to 25 days. The mRNA expression level and cytochrome P450 (CYP1A/EROD (7-Ethoxylesorufin O-deethylase)) and glutathione S-transferase (GST) activity were measured in carp liver and brain tissue. The results showed that PHE stress induced low-concentration induction and high-concentration inhibition of CYP1A expression and EROD enzyme activity in the liver and brain of carp. In both two organs, GST enzyme activity was also induced. However, the expression of GST mRNA was first induced and then inhibited, after the 15th day. These results indicate that long-term exposure to PHE at lower than safe concentrations still poses a potential threat to carp’s oxidase system and gene expression.

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The emerging contaminant 3,3′-dichlorobiphenyl (PCB-11) impedes Ahr activation and Cyp1a activity to modify embryotoxicity of Ahr ligands in the zebrafish embryo model (Danio rerio)

Cited by 22 publications

References 62 publications

Mepanipyrim and cyprodinil induce divergent temporal patterns of AhR-mediated responses in zebrafish (Danio rerio) embryos and larvae

Mepanipyrim and cyprodinil induce divergent temporal patterns of AhR-mediated responses in zebrafish (Danio rerio) embryos and larvae

Hepatic Burdens of PCB and PCDD/F Congeners in Federally Endangered Shortnose Sturgeon and Atlantic Sturgeon from the Hudson River, New York, USA: Burden Patterns and Potential Consequences in Offspring

Long-Term Exposure to Phenanthrene Induced Gene Expressions and Enzyme Activities of Cyprinus carpio below the Safe Concentration

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