The epimastigote stage-specific gene expression of CESP is tightly regulated by its 3′ UTR

Suganuma, Keisuke; Yamasaki, Shino; Asada, Masahito; Kawazu, Shin-ichiro; Inoue, Noboru

doi:10.1016/j.molbiopara.2012.09.010

Cited by 4 publications

(1 citation statement)

References 20 publications

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“…Accumulating evidence indicates that the regulation of gene expression in trypanosomatids is determined at the posttranscriptional level, and several studies have demonstrated that 3'-UTRs play major roles in the control of mRNA stability and the protein translation rate (Abanades, et al, 2009, Blattner and Clayton, 1995, De Gaudenzi, et al, 2013, Pérez-Díaz, et al, 2013, Suganuma, et al, 2012. Fewer reports are available that indicate that 5'-UTR elements and in some cases intergenic regions (IR) also play roles in posttranscriptional mechanisms of gene expression regulation (Jaeger andBrandao, 2011, Mahmood andRay, 1998).…”

Section: Majormentioning

confidence: 99%

Leishmania major phosphoglycerate kinase transcript and protein stability contributes to differences in isoform expression levels

Azevedo

Toledo

Pedrosa

et al. 2015

Experimental Parasitology

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Leishmania contains two phosphoglycerate kinase (PGK) genes, PGKB and PGKC, which code for the cytosolic and glycosomal isoforms of the enzyme, respectively. Although differences in PGKB and PGKC transcript and protein levels and isoform activities have been well documented, the mechanisms of control of both transcript and protein abundance have not been described to date. To better understand the regulation of Leishmania PGK expression, we investigated the stabilities of both PGK transcripts using reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR) in combination with transcription and trans-splicing inhibitors. Cells were treated with sinefungin and actinomycin D, and RNA decay kinetics were assessed. In addition, immunoblotting and protein synthesis inhibition by cycloheximide were employed to evaluate protein steady states and degradation. We observed increased stabilities of both PGKB mRNA and protein compared with the glycosomal isoform (PGKC). Our results indicate that both post-transcriptional and post-translational events contribute to the distinct expression levels of the PGKB and PGKC isoforms in Leishmania major.

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