The establishment of a sensitive method in determining different neurotransmitters simultaneously in rat brains by using liquid chromatography–electrospray tandem mass spectrometry

Validated methods for determination of neurotransmitters and metabolites in rodent brain tissue and extracellular fluid by reversed phase UHPLC–MS/MS

“…However, many of the methods for determination of NTs and metabolites include time consuming derivatization steps [14,18,19] or freeze drying [20], the use of ion pair chromatography which is unfavorable using MS [11,12,28] or relatively long analysis times (10-20 min) [7,[14][15][16][17]. When comparing the amount of brain tissue needed for NT determination, the method in the present study requires very small amounts of tissue (150 g/analysis) as compared to other methods (500-5000 g) [12,15,16,19]. This is advantageous when studying a number of different brain areas from the same animal.…”

“…Previously reported methods for determination of NTs and NT metabolites have displayed long analysis times (10-19.5 min) [7,[14][15][16][17] or time consuming sample preparation including derivatization [14,18,19] or freeze drying [20]. However, methods employing derivatization and freeze drying have been reported to give advantages such as low limits of quantification [18,20].…”

FTY720 Attenuates 6-OHDA-Associated Dopaminergic Degeneration in Cellular and Mouse Parkinsonian Models

“…The striatal dopamine (DA) and its metabolites, dihydroxyphnylacetic acid (DOPAC) and homovanillic acid (HVA) were measured using a highly sensitive High-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method as previously described [12].…”

Schisandrin B shows neuroprotective effect in 6-OHDA-induced Parkinson’s disease via inhibiting the negative modulation of miR-34a on Nrf2 pathway

“…Briefly, the striata of mice were removed at 2 weeks following 6-OHDA administration, then individually weighed and homogenized in ice-cold 0.5 M formic acid at 5 ml/g tissue. Lysates were centrifuged at 15,000 Â g for 30 min at 4 C. The supernatant was separated and analyzed according to established protocols [22].…”