The Evaluation of Antioxidant Capacity of Different Fractions of Myrtus communis L. Leaves

Benchikh, Fatima; Amira, Smain; Benabdallah, Hassiba

doi:10.9734/arrb/2018/39217

Cited by 19 publications

(14 citation statements)

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“…(IC50= 0.73 and 0.82 mg/mL, respectively). This activity could be related to the richness of these extracts in tannins as proved in our previous study (Benchikh et al, 2018). EAE and CHE extracts showed weak chelating activity (IC50=1.14 and 1.43 mg/mL, respectively).…”

Section: Discussionsupporting

confidence: 68%

Free Radical Scavenging, Metal chelating and Antiperoxidative Activities of M. communis Berries Methanol extract and its Fractions

Benchikh

Benabdallah

Amira

et al. 2022

Turkish JAF Sci.Tech.

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Oxidative stress resulted from free radicals and reactive oxygen species (ROS) are associated with many diseases. Phytotherapy has known a great evolution all the world and some medicinal plants are important remedies of some diseases. Myrtle (Mrytus communis L.) is a plant of Myrtaceae family which is common in the Mediterranean region, with flowering, always green leaves and fruit. This study aims to investigate in vitro antioxidant capacity of Myrtus communis berries methanol extract and its three fractions using five assays: ABTS scavenging radicals, metal chelating, hydrogen peroxide and inhibition of lipid peroxidation assays. EAE extract possessed the highest antioxidant activity in ABTS (EAE (IC50=2.5 µg/ml ) and lipid peroxidation models (90.17%). Whereas, AqE is the most active extract in metal chelating activity (IC50=0.73±0.03 mg/ml) and H2O2 assay. These results support the traditional use of this plant in healthcare and it could be a new source of antioxidant natural drugs.

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Section: Discussionsupporting

confidence: 68%

Free Radical Scavenging, Metal chelating and Antiperoxidative Activities of M. communis Berries Methanol extract and its Fractions

Benchikh

Benabdallah

Amira

et al. 2022

Turkish JAF Sci.Tech.

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“…The antioxidant function prevents the formation of peroxide due to the presence of reductones that have the power to break the chain of free radicals by donating a hydrogen atom, or by reacting with certain peroxide precursors [ 86 , 87 ]. The free radical of linoleic acid is attacked by β-carotene so that it undergoes rapid bleaching as it loses the double bonds, and therefore, its orange color [ 88 ]; the existence of a molecule that plays the role of an antioxidant can inhibit or delay the destruction of the β-carotene molecule by neutralizing the radicals of the linoleic acid formed.…”

Section: Resultsmentioning

confidence: 99%

Artemisia absinthium L. Aqueous and Ethyl Acetate Extracts: Antioxidant Effect and Potential Activity In Vitro and In Vivo against Pancreatic α-Amylase and Intestinal α-Glucosidase

et al. 2022

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Artemisia absinthium L. is one of the plants which has been used in folk medicine for many diseases over many centuries. This study aims to analyze the chemical composition of the Artemisia absinthium ethyl acetate and its aqueous extracts and to evaluate their effect on the pancreatic α-amylase enzyme and the intestinal α-glucosidase enzyme. In this study, the total contents of phenolic compounds, flavonoids, and condensed tannins in ethyl acetate and the aqueous extracts of Artemisia absinthium leaves were determined by using spectrophotometric techniques, then the antioxidant capacity of these extracts was examined using three methods, namely, the DPPH (2, 2-diphenyl-1picrylhydrazyl) free radical scavenging method, the iron reduction method FRAP, and the β-carotene bleaching method. The determination of the chemical composition of the extracts was carried out using high-performance liquid chromatography—the photodiode array detector (HPLC-DAD). These extracts were also evaluated for their ability to inhibit the activity of the pancreatic α-amylase enzyme, as well as the intestinal α-glucosidase enzyme, in vitro and in vivo, thus causing the reduction of blood glucose. The results of this study showed that high polyphenol and flavonoid contents were obtained in ethyl acetate extract with values of 60.34 ± 0.43 mg GAE/g and 25.842 ± 0.241 mg QE/g, respectively, compared to the aqueous extract. The results indicated that the aqueous extract had a higher condensed tannin content (3.070 ± 0.022 mg EC/g) than the ethyl acetate extract (0.987 ± 0.078 mg EC/g). Ethyl acetate extract showed good DPPH radical scavenging and iron reduction FRAP activity, with an IC50 of 0.167 ± 0.004 mg/mL and 0.923 ± 0.0283 mg/mL, respectively. The β-carotene test indicated that the aqueous and ethyl acetate extracts were able to delay the decoloration of β-carotene with an inhibition of 48.7% and 48.3%, respectively, which may mean that the extracts have antioxidant activity. HPLC analysis revealed the presence of naringenin and caffeic acid as major products in AQE and EAE, respectively. Indeed, this study showed that the aqueous and ethyl acetate extracts significantly inhibited the pancreatic α-amylase and intestinal α-glucosidase, in vitro. To confirm this result, the inhibitory effect of these plant extracts on the enzymes has been evaluated in vivo. Oral intake of the aqueous extract significantly attenuated starch- and sucrose-induced hyperglycemia in normal rats, and evidently, in STZ-diabetic rats as well. The ethyl acetate extract had no inhibitory activity against the intestinal α-glucosidase enzyme in vivo. The antioxidant and the enzyme inhibitory effects may be related to the presence of naringenin and caffeic acid or their synergistic effect with the other compounds in the extracts.

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“…An aqueous extract of Pituranthos scoparis bark precipitated hemoglobin from blood sample hemolysates [ 21 ]. In separate studies, extracts of Phlomis bovei De, Hertia cleirifolia , Cucumis melo , and Myrtus communis precipitated proteins in bovine blood [ 22 – 25 ]. Previous studies have linked plant and animal-derived enzymes (proteases) and phytochemicals of plant origin, especially phenolics, terpenoids, and organonitrades, to protein precipitating ability.…”

Section: Discussionmentioning

confidence: 99%

Hemoglobin Precipitation: An Index of In Vitro Vasoconstrictive Activities of Methanol Leaf Extracts of Croton megalocarpus Hutch and Lantana camara Linn

Muindi

Kibiti

Ngugi

2021

Evidence-Based Complementary and Alternative Medicine

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The function of innate hemostasis aids the body in bleeding control, preventing the loss of excessive amounts of blood following low-degree injuries. However, injuries of a higher degree may require extrinsic intervention to stop life-threatening blood loss. Astringent agents’ actions result in mechanical constriction of small blood vessels and shrinkage of body tissues, thereby stopping blood loss. This enhances the primary phase of hemostasis, where vasoconstriction is the main mechanism at play during the initial response to injury. The effects of plant extracts on protein precipitation have been linked to blood vessel vasoconstriction. Traditionally, the leaves of Croton megalocarpus Hutch and Lantana camara Linn plants are used by communities living in Makueni County, Kenya, for peripheral bleeding control. However, the effects of extracts of both plants on hemoglobin precipitation have not been evaluated scientifically. In the current study, the activities of methanol extracts of C. megalocarpus (H.) and L. camara (L.) on blood protein precipitation were investigated. The leaves were harvested, cleaned, air-dried, milled, and extracted in absolute methanol before being concentrated into dry powders. A qualitative phytochemical screen revealed the presence of terpenoids, steroids, tannins, phenols, flavonoids, reducing sugars, cardiac glycosides, and carbohydrates in the methanol extract of C. megalocarpus (H.). The methanol extracts of L. camara (L.) contained cardiac glycosides, saponins, tannins, phenols, terpenoids, reducing sugars, and carbohydrates. The hemoglobin precipitation ability of various concentrations of extracts using mice samples was presented as relative astringency following the tannic acid external standard method. Methanol extracts C. megalocarpus (H.) and L. camara (L.) had significantly higher relative astringency compared with the normal control, indicating a protein precipitating activity. The relative astringency observed in both plant extracts is linked to the activity of tannins, phenols, flavonoids, and saponins detected during preliminary phytochemical screening.

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The Evaluation of Antioxidant Capacity of Different Fractions of Myrtus communis L. Leaves

Cited by 19 publications

References 0 publications

Free Radical Scavenging, Metal chelating and Antiperoxidative Activities of M. communis Berries Methanol extract and its Fractions

Free Radical Scavenging, Metal chelating and Antiperoxidative Activities of M. communis Berries Methanol extract and its Fractions

Artemisia absinthium L. Aqueous and Ethyl Acetate Extracts: Antioxidant Effect and Potential Activity In Vitro and In Vivo against Pancreatic α-Amylase and Intestinal α-Glucosidase

Hemoglobin Precipitation: An Index of In Vitro Vasoconstrictive Activities of Methanol Leaf Extracts of Croton megalocarpus Hutch and Lantana camara Linn

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