The Evaluation of the Possibilities of Using PLGA Co-Polymer and Its Composites with Carbon Fibers or Hydroxyapatite in the Bone Tissue Regeneration Process – in Vitro and in Vivo Examinations

Cieślik, M.; Mertas, Anna; Morawska-Chochół, A.; Sabat, D.; Orlicki, R; Owczarek, Aleksander; Król, Wojciech; Cieślik, T.

doi:10.3390/ijms10073224

Cited by 30 publications

(27 citation statements)

References 26 publications

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“…2a). The bioactive activated carbon fibers are biocompatible and have been used to create 3D scaffolds for cell growth, proliferation, and adhesion in tissue engineering [20]. Figure 4 shows the morphology of RSF cells attached to the surface and interior pore space of activated carbon fibers at different times during cell culturing.…”

Section: Discussionmentioning

confidence: 99%

“…They are also used for the purification of various biofluids, including the cleansing blood of inflammatory mediators under conditions such as sepsis or remove cytokines interleukin-6 (IL-6), interleukin-8 (IL-8), and tumour necrosis factoralpha (TNF-a) [17][18][19]. Additionally, activated carbon fibers are biocompatible and bioactive, making them ideal for 3D scaffolds for cell growth, proliferation, or tissue engineering [20,21].…”

Section: Introductionmentioning

confidence: 99%

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Development of fibroblast culture in three-dimensional activated carbon fiber-based scaffold for wound healing

Huang

Yeh

Lin³

et al. 2012

J Mater Sci: Mater Med

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This work developed a novel bi-layer wound dressing composed of 3D activated carbon fibers that allows facilitates fibroblast cell growth and migration to a wound site for tissue reconstruction, and the gentamicin is incorporated into a poly(γ-glutamic acid)/gelatin membrane to prevent bacterial infection. In an in vitro, field emission scanning electron microscopy shows that rat skin fibroblasts appeared and spread on the surface of activated carbon fibers, and penetrated the interior and exterior of the 3D activated carbon fiber construct to a depth of roughly 200 μm. An in vivo analysis shows that fibroblast cells containing the proposed 3D scaffold had the potential of a biologically functionalized dressing to accelerate wound closure. Additionally, fibroblasts migrated to the wound site in a bi-layer wound dressing containing fibroblasts, enhancing fibronectin and type I collagen expression, resulting in faster skin regeneration than that achieved with a Tegaderm™ hydrocolloid dressing or gauze.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Development of fibroblast culture in three-dimensional activated carbon fiber-based scaffold for wound healing

Huang

Yeh

Lin³

et al. 2012

J Mater Sci: Mater Med

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“…The use of sequentially combined cell viability parameters during a cytotoxicity assay increases the chances of the detection of adverse effects and contributes to the refinement of the understanding of the mechanisms involved on toxicity (16). In this context, other studies of PLGA/HA composites found similar good cell viability by employing other non-metabolic assays, such as the LDH test with human hFOb 1.19 osteoblast-like cells (17), or through fluorescence-based LIVE-DEAD tests with RAW 264.7 murine macrophages (18). Moreover, the in vitro evaluation of biocompatibility of the PLGA/HA composite scaffolds Usually, during the development of novel biomaterials, in vitro results pointing to strong cytotoxic effects, such as those identified for the ReOss putty configuration (Fig.…”

Section: In Vitro and In Vivo Biocompatibility Of Reoss® Hydroxyapatitementioning

confidence: 97%

“…In the literature, favorable in vivo results were reported for the biological response for PLGA:HA composites as bone substitutes (13,17,20), as authors observed osteogenesis, and no inflammatory persistent reactions or osteolysis in implant sites. A PLGA/HA nanostructured composite, manufactured by baryosynthesis route, the same route described for the ReOss ® , resulted in a composite with enhanced biocompatibility and osteogenic properties compared to a similar composite fabricated by the solvent casting/particulate leaching process (3).…”

Section: In Vitro and In Vivo Biocompatibility Of Reoss® Hydroxyapatitementioning

confidence: 99%

In Vitro and In Vivo Biocompatibility Of ReOss® in Powder and Putty Configurations

et al. 2018

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This study evaluated comparatively two configurations (powder and putty) of a composite biomaterial based on PLGA (Poly(lactide-co-glycolide)/nanoescale hydroxyapatite (ReOss®, Intra-Lock International) through microscopic morphology, in vitro cytotoxicity, biocompatibility and in vivo response as a bone substitute. SEM and EDS characterized the biomaterials before/after grafting. Cytocompatibility was assessed with murine pre-osteoblasts. Osteoconductivity and biocompatibility were evaluated in White New Zealand rabbits. Both configurations were implanted in the calvaria of eighteen animals in non-critical size defects, with blood clot as the control group. After 30, 60 and 90 days, the animals were euthanized and the fragments containing the biomaterials and controls were harvested. Bone blocks were embedded in paraffin (n=15) aiming at histological and histomorphometric analysis, and in resin (n=3) aiming at SEM and EDS. Before implantation, the putty configuration showed both a porous and a fibrous morphological phase. Powder revealed porous particles with variable granulometry. EDS showed calcium, carbon, and oxygen in putty configuration, while powder also showed phosphorus. After implantation EDS revealed calcium, carbon, and oxygen in both configurations. The materials were considered cytotoxic by the XTT test. Histological analysis showed new bone formation and no inflammatory reaction at implant sites. However, the histomorphometric analysis indicated that the amount of newly formed bone was not statistically different between experimental groups. Although both materials presented in vitro cytotoxicity, they were biocompatible and osteoconductive. The configuration of ReOss® affected morphological characteristics and the in vitro cytocompatibility but did not impact on the in vivo biological response, as measured by the present model.

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“…Moreover, in their previous papers, the authors proved a stimulating effect of carbon fibres on the bone growth processes [17][18][19]. Moreover, in their previous papers, the authors proved a stimulating effect of carbon fibres on the bone growth processes [17][18][19].…”

Section: Introductionmentioning

confidence: 99%

Magnesium alloy wires as reinforcement in composite intramedullary nails

Morawska-Chochół

Chłopek

Domalik-Pyzik

et al. 2014

Bio-Medical Materials and Engineering

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A promising group of biomaterials assigned for the production of intramedullary nails are composites with a polylactide (PLA) matrix, reinforced with wires made of magnesium alloys and carbon fibres. The paper describes the effect of the composition of magnesium alloy wires, their number and orientation in the composite, as well as their connection with differently directed long carbon fibres, on the mechanical properties and the degradation rate of the obtained intramedullary nails. Among the tested implant prototypes, the best mechanical characteristics and a gradual and uniform course of magnesium alloy wires were exhibited by the PLA + CF1D + MgI composite nails (with a unidirectional orientation of carbon fibres and an axially oriented single Mg alloy wire). The strength of these nails became gradually decreased with the incubation time, which should allow for a gradual loading of the bone. In the case of the PLA with only magnesium alloy wires (without carbon fibres), the increase of the number of wires, on the one hand, stimulates the improvement of the nails' strength, yet on the other hand, a higher content of magnesium alloys in the PLA matrix affects the nails' faster resorption.

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The Evaluation of the Possibilities of Using PLGA Co-Polymer and Its Composites with Carbon Fibers or Hydroxyapatite in the Bone Tissue Regeneration Process – in Vitro and in Vivo Examinations

Cited by 30 publications

References 26 publications

Development of fibroblast culture in three-dimensional activated carbon fiber-based scaffold for wound healing

Development of fibroblast culture in three-dimensional activated carbon fiber-based scaffold for wound healing

In Vitro and In Vivo Biocompatibility Of ReOss® in Powder and Putty Configurations

Magnesium alloy wires as reinforcement in composite intramedullary nails

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