The examination of skin lipid model systems stressed by ultraviolet irradiation in the presence of transition metal ions

Trommer, Hagen; Wagner, Jens; Graener, H.; Neubert, Reinhard H.H.

doi:10.1016/s0939-6411(01)00140-0

Cited by 28 publications

(20 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Recently, we have shown by means of the thiobarbituric acid assay (detecting malondialdehyde as a classic secondary product of lipid peroxidation) that the encapsulation of fatty acids into liposomes made fatty acids more sensitive to oxidative damage [25]. These results made by quantification of the lipid peroxidation amount could be confirmed in this study in a qualitative manner by means of MS. Figure 6 shows the negative ion mode mass spectrum of LLA exposed for 120 min to UV irradiation without iron ions ( fig.…”

Section: Liposomal Systemssupporting

confidence: 76%

“…The pseudomolecular [M-H] -ion of LLA was still visible, but the intensity of the LLA peroxidation products was increased. An explanation could be the close arrangement of the lipids allowing a chain reaction to propagate a different absorption behaviour of the encapsulated fatty acid with an absorbance shift to a higher wavelength as discussed in Trommer et al [25].…”

Section: Liposomal Systemsmentioning

confidence: 99%

See 1 more Smart Citation

Mass Spectrometric Examinations of Stratum corneum Lipid Models Exposed to Ultraviolet Irradiation

Trommer

Plätzer

Wolf

et al. 2003

Skin Pharmacol Physiol

View full text Add to dashboard Cite

Lipid model systems consisting of the major components of the stratum corneum intercellular lipid matrix were studied to investigate the ultraviolet-radiation-mediated damage of these biomolecules. Pure lipids and liposomes were irradiated using a lamp emitting a solar radiation spectrum. The influences of the irradiation and the effects of added iron ions were studied by electrospray ionization mass spectrometry (MS) with an ion trap analyser. Exact mass measurements were carried out using a time-of-flight mass spectrometer. Only linolenic acid and cholesterol were found to be subject to oxidative changes caused by UV irradiation whereas the other lipids examined (dipalmitoylphosphatidylcholine, ceramide III and cholesterol sulphate) were stable to oxidative stress. Several lipid adducts were observed upon analysis of the liposomes. The composition of these adducts was identified by MS/MS experiments.

show abstract

Section: Liposomal Systemssupporting

confidence: 76%

Section: Liposomal Systemsmentioning

confidence: 99%

Mass Spectrometric Examinations of Stratum corneum Lipid Models Exposed to Ultraviolet Irradiation

Trommer

Plätzer

Wolf

et al. 2003

Skin Pharmacol Physiol

View full text Add to dashboard Cite

show abstract

“…Gruber and co‐authors reported that the UVA‐1‐induced photooxidation of PAPC (1‐palmitoyl‐2‐arachidonoyl‐ sn ‐glycero‐3‐phosphocholine) leads to the formation of long chain oxidation products, including hydroperoxides, a trace amount of hydroxides and isoprostane‐like modifications as the epoxyisoprostane‐phosphatidylcholine derivative . Trommer and co‐authors investigated the effects of UVA radiation on lipid peroxidation of different systems including fatty acids and phosphatidylcholine, in the presence of ionized iron using ESI‐MS and identified hydro‐ and endoperoxides oxidation products . Gugiu and co‐authors reported the in vitro oxidation of phosphatidylethanolamine (PE), using different oxidation systems including UV, with formation of truncated PEs.…”

Section: Introductionmentioning

confidence: 99%

Photooxidation of glycated and non‐glycated phosphatidylethanolamines monitored by mass spectrometry

et al. 2013

View full text Add to dashboard Cite

Phosphatidylethanolamines (PE) are one of the major components of cells membranes, namely in skin and in retina, that are continuously exposed to solar UV radiation being major targets of photooxidation damage. In addition, due to the presence of the free amine group, PE can also undergo glycation, in hyperglycemic conditions which may increase the susceptibility to oxidation. The aim of this study is to develop a model, based on mass spectrometry (MS) analysis, to identify photooxidative degradation of selected PE (POPE: PE 16:0/18:1, PLPE: PE 16:0/18:2, PAPE: PE 16:0/20:4) and glycated PEs due to UV irradiation. Photooxidation products were analysed by electrospray ionization MS (ESI-MS) and tandem MS (ESI-MS/MS) in positive and negative mode. Emphasis is placed in the influence of glycation in the generation of distinct photooxidation products. ESI-MS spectra of PE after UV photo-irradiation showed mainly hydroperoxy derivatives, due to oxidation of unsaturated fatty acyl chains. Glycated PE gave rise to several new photooxidation products formed due to oxidative cleavages of the glucose moiety, namely between C1 and C2, C2 and C3, and C5 and C6 of this sugar unit. These new products were identified by ESI-MS/MS in positive mode showing distinct neutral loss depending on the different structure of the polar head group. These new identified advanced glycated photooxidation products may have a deleterious role in the etiology of diabetic retinopathy and in diabetic retinal microvascular complications.

show abstract

“…Fatty acids are essential components of natural lipids, which determine the physiological structure and function of the human skin (3). They are present in the epidermis, especially in the stratum corneum, the outermost layer, and cell membranes (4).…”

mentioning

confidence: 99%

Skin Aging and Photoaging Alter Fatty Acids Composition, Including 11,14,17-eicosatrienoic Acid, in the Epidermis of Human Skin

Kim

Jin

et al. 2010

J Korean Med Sci

View full text Add to dashboard Cite

We investigated the alterations of major fatty acid components in epidermis by natural aging and photoaging processes, and by acute ultraviolet (UV) irradiation in human skin. Interestingly, we found that 11,14,17-eicosatrienoic acid (ETA), which is one of the omega-3 polyunsaturated acids, was significantly increased in photoaged human epidermis in vivo and also in the acutely UV-irradiated human skin in vivo, while it was significantly decreased in intrinsically aged human epidermis. The increased ETA content in the epidermis of photoaged human skin and acute UV-irradiated human skin is associated with enhanced expression of human elongase 1 and calcium-independent phophodiesterase A2. We demonstrated that ETA inhibited matrix metalloproteinase (MMP)-1 expression after UV-irradiation, and that inhibition of ETA synthesis using EPTC and NA-TCA, which are elongase inhibitors, increased MMP-1 expression. Therefore, our results suggest that the UV increases the ETA levels, which may have a photoprotective effect in the human skin.

show abstract

The examination of skin lipid model systems stressed by ultraviolet irradiation in the presence of transition metal ions

Cited by 28 publications

References 24 publications

Mass Spectrometric Examinations of Stratum corneum Lipid Models Exposed to Ultraviolet Irradiation

Mass Spectrometric Examinations of Stratum corneum Lipid Models Exposed to Ultraviolet Irradiation

Photooxidation of glycated and non‐glycated phosphatidylethanolamines monitored by mass spectrometry

Skin Aging and Photoaging Alter Fatty Acids Composition, Including 11,14,17-eicosatrienoic Acid, in the Epidermis of Human Skin

Contact Info

Product

Resources

About