The expression of microRNA 574-3p as a predictor of postoperative outcome in patients with esophageal squamous cell carcinoma

Abstract

BackgroundDespite advances in radical esophagectomies and adjuvant therapy, the postoperative prognosis in esophageal squamous cell carcinoma (ESCC) patients remains poor. The aim of this study was to identify a molecular signature to predict postoperative favorable outcomes in patients with ESCC.MethodsAs a training data set, total RNA was extracted from formalin-fixed paraffin-embedded samples of surgically removed specimens from 19 ESCC patients who underwent curative esophagectomy. The expression of microRNA (miRNA) was detected using a miRNA oligo chip on which 885 genes were mounted. As a validation data set, we obtained frozen samples of surgically resected tumors from 12 independent ESCC patients and the expression of miR-574-3p was detected by quantitative real-time PCR.ResultsOur microarray analysis in the training set patients identified three miRNAs (miR-574-3p, miR-106b, and miR-1303) and five miRNAs (miR-1203, miR-1909, miR-204, miR-371-3p, miR-886-3p) which were differentially expressed between the patients with (n = 14) and without (n = 5) postoperative tumor relapse (p < 0.01 and p < 0.05, respectively). Higher expression of miR-574-3p, which showed the most significant association with non-relapse (p = 0.001), was associated with favorable overall survival (p = 0.016). Real-time PCR experiments on the validation set patients confirmed that higher expression of miR-574-3p was associated with non-tumor relapse (p = 0.029) and better overall survival (p = 0.004).ConclusionsOur results suggest that the aberrant expression of the miRNAs identified in this study plays key roles in the progression of ESCC. miR-574-3p was suggested to have a tumor suppressor effect, and thus, to be a predictor of postoperative outcome in patients with ESCC.Electronic supplementary materialThe online version of this article (doi:10.1186/s12957-016-0985-3) contains supplementary material, which is available to authorized users.

vtRNA2-1/nc886 Produces a Small RNA That Contributes to Its Tumor Suppression Action through the microRNA Pathway in Prostate Cancer

“…In the prostate, the loss of expression of hsa-miR-886-3p was associated with early biochemical relapse (<1 year after radical prostatectomy) [20], but a recent study shows an increased abundance of circulating hsa-miR-886-3p in high-grade compared to low-grade prostate cancer in plasma samples [32]. Notwithstanding, few reports showed an oncogenic action of hsa-miR-886-3p in renal cell carcinoma [33], colorectal carcinoma [34] and esophageal squamous cell carcinoma [35]. A recent finding proposing a nc886/PKR loss mediated doxorubicin cytotoxicity raised a novel view about its specific contribution to chemotherapy response [36].…”

Overexpression of miR‑574‑3p suppresses proliferation and induces apoptosis of chronic myeloid leukemia cells via targeting IL6/JAK/STAT3 pathway

“…These data imply that miR-574-3p may exert a tumor suppressor role in K562 cells via regulating IL6/JAK/STAT3 pathway. In previous studies, miR-574-3p has a tumor-promoting role in human osteosarcoma (18), while exerts a tumor suppressor effect in esophageal squamous cell carcinoma (19), indicating that miR-574-3p may play a dual role in regulating cancer progression. Furthermore, it is reported that aberrantly expression of miR-574-3p may play functional roles in regulating the proliferation and metatasis of gastric cancer (16).…”

Prognostic Value of MicroRNAs in Esophageal Carcinoma: A Meta-Analysis

“…The forest plots, Begg’s funnel plots, and sensitivity analyses are shown in Supplementary Figures 1 – 8 according to the logic sequencing of miRNA names. For the included 96 studies, 52 were excluded because the frequency of them evaluating prognostic value of miRNA was equal to 1 3 , 4 , 16 , 18 – 23 , 26 , 30 , 31 , 39 – 46 , 48 , 53 , 54 , 56 , 60 , 61 , 64 – 74 , 77 – 86 , 92 – 96 . In addition, although one article reported OS results about miR-193a-5p, it was excluded because it had non-dichotomous miRNA expression value 105 .…”