The expression of myosin genes in developing skeletal muscle in the mouse embryo.

Lyons, Gary E.; Ontell, Marcia; Cox, Roger; Sassoon, David; Buckingham, Margaret

doi:10.1083/jcb.111.4.1465

Cited by 260 publications

(220 citation statements)

References 36 publications

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“…However, Dusterhoft and Pette (1993) used satellite cells isolated from the slow Soleus muscle that are more likely to express slow MHC isoforms (MHC1 and MHC2a) in culture, as we also showed here for clones isolated from Soleus muscle. The expression of the MHC1 isoform observed in all our cultured cell lines might reflect a natural process occurring in vivo during myogenesis: in chick (Sacks et al, 2003) as in mouse (Lyons et al, 1990), the slow MHC isoform is expressed together with developmental MHC isoforms in the embryonic muscles. The separation between fast and slow myofibers appears to occur later, in secondary fibers (Lyons et al, 1990).…”

Section: Defined Conditions For Optimal Differentiation Of the Neonatmentioning

confidence: 87%

“…The expression of the MHC1 isoform observed in all our cultured cell lines might reflect a natural process occurring in vivo during myogenesis: in chick (Sacks et al, 2003) as in mouse (Lyons et al, 1990), the slow MHC isoform is expressed together with developmental MHC isoforms in the embryonic muscles. The separation between fast and slow myofibers appears to occur later, in secondary fibers (Lyons et al, 1990).…”

Section: Defined Conditions For Optimal Differentiation Of the Neonatmentioning

confidence: 87%

“…As myotube formation and differentiation is not completely synchronous, this variation in expression levels of MHC isoforms may represent myotubes at slightly different stages of differentiation. In vivo expression of several adult MHC isoforms in the so-called hybrid fibers has been reported as indicative of a change in phenotype occurring in adult striated muscle (Staron and Pette, 1993;Caiozzo et al, 2003), and during embryogenesis as well (Lyons et al, 1990).…”

Section: Double Labeling Immunocytochemical Analysis Of Wtt Myofibersmentioning

confidence: 99%

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Novel murine clonal cell lines either express slow or mixed (fast and slow) muscle markers following differentiation in vitro

Peltzer

Colman

Cebrián

et al. 2008

Developmental Dynamics

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We have investigated whether the phenotype of myogenic clones derived from satellite cells of different muscles from the transgenic immortomouse depended on muscle type origin. Clones derived from neonatal, or 6-to 12-week-old fast and slow muscles, were analyzed for myosin and enolase isoforms as phenotypic markers. All clones derived from slow-oxidative muscles differentiated into myotubes with a preferentially slow contractile phenotype, whereas some clones derived from rapid-glycolytic or neonatal muscles expressed both fast and slow myosin isoforms. Thus, muscle origin appears to bias myosin isoform expression in myotubes. The neonatal clone (WTt) was cultivated in various medium and substrate conditions, allowing us to determine optimized conditions for their differentiation. Matrigel allowed expressions of adult myosin isoforms, and an isozymic switch from embryonic ␣-toward muscle-specific ␤-enolase, never previously observed in vitro. These cells will be a useful model for in vitro studies of muscle fiber maturation and plasticity.

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Section: Defined Conditions For Optimal Differentiation Of the Neonatmentioning

confidence: 87%

Section: Defined Conditions For Optimal Differentiation Of the Neonatmentioning

confidence: 87%

Section: Double Labeling Immunocytochemical Analysis Of Wtt Myofibersmentioning

confidence: 99%

See 1 more Smart Citation

Novel murine clonal cell lines either express slow or mixed (fast and slow) muscle markers following differentiation in vitro

Peltzer

Colman

Cebrián

et al. 2008

Developmental Dynamics

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“…The procedure for in situ hybridisation used was similar to that reported by Lyons et al [27] with slight modifications. Briefly, frozen cross-sections of muscle and liver tissues were made using a 2800 Frigocut microtome (Reichert-Jung, Lahntechnik, Germany) and mounted on gelatincoated (0.2 % w/v gelatin) slides.…”

Section: In Situ Hybridisationmentioning

confidence: 99%

Growth hormone receptor gene expression in the skeletal muscle of normal and double-muscled bovines during foetal development

Listrat

Hocquette²,

Picard³

et al. 2005

Reprod. Nutr. Dev.

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-The expression of the growth hormone receptor (GHR) gene was investigated in semitendinosus muscle during bovine foetal development in both normal and double-muscled Charolais foetuses which differ with respect to muscle development. Northern-blot analysis of foetal muscle RNA preparations with a GHR cDNA probe identified the 4.5 kb GHR mRNA as early as 130 days post-conception. In double-muscled animals, the expression of GHR mRNA increased from 130 to 210 days of gestation while it stayed stable in normal ones. It was significantly higher (P < 0.05) in double-muscled foetuses compared to normal ones from the second third of gestation. Northern-blot analysis of foetal muscle RNA preparations from both genotypes with a β-actin cDNA probe, revealed lower β-actin gene expression in double-muscled foetuses than in normal ones, suggesting a delay in the differentiation of muscle cells. In situ hybridisation revealed the localisation of specific GHR mRNA in muscle cells at all gestation stages analysed (130, 170, 210 days postconception) but not in connective tissue surrounding the muscle cells. At the adult stage, the hybridisation signal was also very high and observed in muscle cells only. These results show the ontogeny of GHR mRNA in bovine muscle and demonstrate a difference between normal and doublemuscled animals. muscle / bovine / receptor / growth hormone / in situ hybridisation

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“…Recently, the localization of slow and fast myosin isoforms in the myotome was determined indicating the spatial relationship of older myotome fibers (Sacks et al, 2003). The localized transcription of the muscle determination factor genes in the somite has been studied extensively (Sassoon et al, 1989;de la Brousse and Emerson, 1990;Lyons et al, 1990;Ott et al, 1991;Pownall and Emerson, 1992;Kiefer and Hauschka, 2001;Pownall et al, 2002;Summerbell et al, 2002) and the localization of their cognate proteins has been documented by immunolocalization (Cusella-De Angelis et al, 1992;Smith et al, 1994;Venters et al, 1999). No previous study, however, provided a systematic correlation between the localization of contractile and regulatory proteins and the birth and progressive maturation of nascent myoblasts during the expansion of the epaxial primary myotome.…”

Section: Introductionmentioning

confidence: 99%

The pattern of MyoD and contractile protein localization in primary epaxial myotome reflects the dynamic progression of nascent myoblast differentiation

Yang

Ordahl

2005

Developmental Dynamics

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The localization of contractile and regulatory proteins in early stages of epaxial primary myotome development was analyzed by immunofluorescence microscopy. Contractile proteins that appear in an ordered sequence in the rostro-caudal axis of somite development were found to reiterate that sequence in the dorso-medial-to-ventro-lateral axis of primary epaxial myotome development. Pair-wise localization of MyoD-titin, desmin-titin, and desmin-myosin defined three zones extending from the dermomyotome dorso-medial lip (DML) into the primary myotome layer. Zones M1 and M2, which were positive for MyoD ؉ titin and MyoD ؉ titin ؉ desmin, respectively, were restricted to the dorso-medial-most extremity of the myotome layer and did not expand during the course of myotome development. Zone M3 was positive for MyoD, desmin, titin, myosin, and cardiac troponin T and was the only zone that expanded during primary myotome development. Myotome fibers in zone M3 were unit-length, spanning the full rostro-caudal axis of the myotome while fibers in zones M1 and M2 were shorter than unit length. Anti-myoD immunofluorescence, when detected in cells lacking contractile-protein-positive cytoplasm, was restricted to the DML and nascent myotome cells immediately subjacent to the DML. These results demonstrate a dynamic spatiotemporal sequence in the differentiation program of nascent myotome cells as they emerge from the DML; zones M1 and M2 reflect standing waves of sequential contractile protein activation during the maturation of nascent myotomal myoblasts, while the expanding zone M3 reflects the accumulation of mature myotome fibers expressing a full cohort contractile proteins. Developmental Dynamics 235:382-394, 2006.

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The expression of myosin genes in developing skeletal muscle in the mouse embryo.

Abstract: Abstract. Using in situ hybridization, we have investigated the temporal sequence of myosin gene expression in the developing skeletal muscle masses of mouse embryos. The probes used were isoformspecific,

Cited by 260 publications

References 36 publications

Novel murine clonal cell lines either express slow or mixed (fast and slow) muscle markers following differentiation in vitro

Novel murine clonal cell lines either express slow or mixed (fast and slow) muscle markers following differentiation in vitro

Growth hormone receptor gene expression in the skeletal muscle of normal and double-muscled bovines during foetal development

The pattern of MyoD and contractile protein localization in primary epaxial myotome reflects the dynamic progression of nascent myoblast differentiation

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