The fate of poly(2-dimethyl amino ethyl)methacrylate-based polyplexes after intravenous administration

Characterization of Tailor-Made Copolymers of Oligo(ethylene glycol) Methyl Ether Methacrylate and N,N-Dimethylaminoethyl Methacrylate as Nonviral Gene Transfer Agents: Influence of Macromolecular Structure on Gene Vector Particle Properties and Transfection Efficiency

“…13,14 In fact, it has been previously reported that tissue distribution of cationic gene vectors, especially the high transfection efficiency of P(D-MAEMA) or PEI in the lungs, can be ascribed to the formation of aggregates formed with blood cells and plasma proteins after intravenous administration followed by enhanced deposition in the capillary bed of the lungs (physical trapping). 4,[15][16][17] On the other hand, a positive charge and a small size are critical for cell surface binding and internalization, which most likely takes place via nonspecific adsorptive endocytosis. 18,19 Moreover, relatively high concentrations of pDNA are indispensable for in vivo gene vector application.…”

Characterization and in vivo performance of dextran–spermine polyplexes and DOTAP/cholesterol lipoplexes administered locally and systemically

“…However, these non-viral gene delivery systems have several major drawbacks including lack of specificity, cell toxicity, low biodegradability, stability, and especially, low transfection efficiency. In addition, their rapid clearance from the blood circulation limits potential transfection sites to first-pass organs, especially the lungs [2][3][4].…”

Self-assembling Pluronic®-modified polycations in gene delivery

“…Polycations bind to nucleic acids via formation of cooperative systems of salt bonds between the cationic groups of the polycation and phosphate groups of the nucleotides. Polycations such as linear and cross-linked poly(ethylene imine) (PEI) [5,6] or cationic methacrylates [7][8][9] have been shown, using luciferase and other encoding reporter genes, and some therapeutic genes, to be efficient in vivo transfection agents. Polycationic vectors of sufficient colloidal stability with favorable pharmacokinetics, significant circulation time of their complexes with DNA in the bloodstream, and organ distribution profiles have been demonstrated [10 • ].…”