The feasibility of sexing bovine morula stage embryos prior to embryo transfer

Singh, Elizabeth L.; Hare, W.C.D.

doi:10.1016/0093-691x(80)90053-9

Cited by 38 publications

(18 citation statements)

References 10 publications

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“…Small biopsies of trophoectoderm or half of bisected embryos can be used for chromosomal analysis in cattle. However, only about two thirds of embryos can be successfully sexed in this way, due to a low mitotic index of biopsied cells (Singh and Hare, 1980) and to difficulty in preparing analyzable metaphase spreads from more than about that percentage of the embryos.…”

Section: Discussionmentioning

confidence: 99%

Embryo sex selection by a rat male‐specific antibody and the cytogenetic and developmental confirmation in cattle embryos

Utsumi

Hayashi

Takakura

et al. 1993

Molecular Reproduction Devel

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Embryos of mouse, rabbit, goat, sheep, and cattle were separated into 2 groups on the basis of their morphology when incubated with a male-specific antibody (qualified here as the H-Y antibody) prepared from newborn rat testis. When morula-stage embryos were cultured in the presence of this H-Y antibody, the development of roughly one half of the embryos was arrested at that stage, whereas the other half continued to develop to the blastocyst stage. The developmentally arrested group of embryos resumed their development into blastocysts when cultured in antibody-free medium. Eighty to 90% of cattle embryos whose development was unaffected by the antibody were shown to possess a female karyotype (XX), and close to 80% of those embryos whose development was arrested possessed a male karyotype (XY). Cattle embryos whose sex had been presumptively identified by development in the presence of the H-Y antibody were cryopreserved and transferred, and the sex of the calves was examined. The overt sex of the young born from sexed embryos was found to be the same as that determined by chromosomal analysis.

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Section: Discussionmentioning

confidence: 99%

Embryo sex selection by a rat male‐specific antibody and the cytogenetic and developmental confirmation in cattle embryos

Utsumi

Hayashi

Takakura

et al. 1993

Molecular Reproduction Devel

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“…In most other species, including farm animals, this method cannot be applied so simply due to the dense, non transparent cytoplasm. Likewise, cytogenetic methods based on the morphology of X and Y chromosomes (Singh and Hare, 1980;Rall and Leibo, 1987), did not live up to expectations. The same holds true for efforts directed at the immunological determination of specific sex antigen (White et al, 1982;Booman et al, 1989) and the detection of metabolic differences between male and female embryos (Williams, 1986).…”

mentioning

confidence: 99%

Lower quality bovine embryos may be successfully used for sex determination

Lopatářová¹,

Čech²,

Krontorád³

et al. 2007

Vet. Med. - Czech

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AbstrAct:The aim of this study was to evaluate the effect of sex determination procedures in Day 7 to Day 8 bovine embryos of various quality. For the purposes of comparison we used high quality (HQ) as well as lower quality (LQ) embryos obtained from superovulated donors. The healthy embryonic cells isolated from HQ embryos and blastomeres protruding into the perivitelline space of LQ embryos, were analysed by polymerase chain reactions (PCR) using primers specific for the Y chromosome determinant. After microsurgical intervention and completion of sex determination, the female embryos were then transferred (ET) to synchronized recipients. A total of 310 embryos of HQ were used and gender was safely determined in 275 cases (88.7%). PCR analysis of extruded cells isolated from 170 LQ embryos was carried out with certainty only in 111 embryos (65.3%, P < 0.01). After ET of 122 HQ sex defined embryos, pregnancy was established in 69 recipients (56.6%). A similar conception rate 51.9% (27/52) was found after the ET of sex defined embryos designated as LQ. The accuracy of analysis was confirmed after calving and revealed that designated female sex coincided with 95.5% and 96.2% of calves when HQ and LQ embryos were transferred, respectively. Our results clearly show that a microsurgical technique in combination with PCR method represents a rapid and reliable approach for sex determination in HQ as well as in LQ preimplantation bovine embryos and can be used in field conditions for the regulation of the sex of progeny in selected herds.

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“…However, in a subsequent report by Singh and Hare (1980), in which they attempted to sex morula stage cattle embryos, the findings of Moustafa ef a/. (1978) could not be confirmed and the Canadian workers concluded that the very low success rate in sexing week-old cattle embryos did not warrant consideration of freezing sexed embryos at that stage.…”

Section: Sexing the Week-old Embryomentioning

confidence: 97%

Embryo Transfer in Cattle

Gordon

1983

Controlled Breeding in Farm Animals

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The feasibility of sexing bovine morula stage embryos prior to embryo transfer

Cited by 38 publications

References 10 publications

Embryo sex selection by a rat male‐specific antibody and the cytogenetic and developmental confirmation in cattle embryos

Embryo sex selection by a rat male‐specific antibody and the cytogenetic and developmental confirmation in cattle embryos

Lower quality bovine embryos may be successfully used for sex determination

Embryo Transfer in Cattle

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