The feasibility of using dielectrophoresis for isolation of glioblastoma subpopulations with increased stemness

Alinezhadbalalami, Nastaran; Douglas, Temple A.; Balani, Nikita; Verbridge, Scott S.; Davalos, Rafael V.

doi:10.1002/elps.201900026

Cited by 14 publications

(12 citation statements)

References 39 publications

(52 reference statements)

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“…Alinezhadbalalami et al. fabricated a cDEP device with an array of insulating posts, which was used to isolate prostate tumor cells with stem‐like properties from other malignancies, and separate aggressive ovarian cancer cells from early stage cells [106]. Additionally, cells from the U251 glioblastoma cell line and its spheroid counterpart, SF‐U251, were differentiated based on their respective dielectric properties.…”

Section: Biological Applicationsmentioning

confidence: 99%

Dielectrophoresis: Developments and applications from 2010 to 2020

et al. 2020

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The 20th century has seen tremendous innovation of dielectrophoresis (DEP) technologies, with applications being developed in areas ranging from industrial processing to micro‐ and nanoscale biotechnology. From 2010 to present day, there have been 981 publications about DEP. Of over 2600 DEP patents held by the United States Patent and Trademark Office, 106 were filed in 2019 alone. This review focuses on DEP‐based technologies and application developments between 2010 and 2020, with an aim to highlight the progress and to identify potential areas for future research. A major trend over the last 10 years has been the use of DEP techniques for biological and clinical applications. It has been used in various forms on a diverse array of biologically derived molecules and particles to manipulate and study them including proteins, exosomes, bacteria, yeast, stem cells, cancer cells, and blood cells. DEP has also been used to manipulate nano‐ and micron‐sized particles in order to fabricate different structures. The next 10 years are likely to see the increase in DEP‐related patent applications begin to result in a greater level of technology commercialization. Also during this time, innovations in DEP technology will likely be leveraged to continue the existing trend to further biological and medical‐focused applications as well as applications in microfabrication. As a tool leveraged by engineering and imaginative scientific design, DEP offers unique capabilities to manipulate small particles in precise ways that can help solve problems and enable scientific inquiry that cannot be addressed using conventional methods.

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Section: Biological Applicationsmentioning

confidence: 99%

Dielectrophoresis: Developments and applications from 2010 to 2020

et al. 2020

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“…Many lab-on-a-chip designs focus on the separation and concentration of target particles into predictable locations. Other designs include implementations of DEP where exosomes, 14,15 stem cells, 21,22 blood cells, 23,24 cancer cells, [25][26][27] bacteria, 28,29 viruses, 30,31 and synthetic NPs 16,32,33 are all collected in predictable locations. Other collection modes include electrophoretic, 34 magnetophoretic, 35,36 and acoustophoretic [37][38][39] forces to concentrate targets from surrounding media to known regions on the chip.…”

Section: Introductionmentioning

confidence: 99%

Automated fluorescence quantification of extracellular vesicles collected from blood plasma using dielectrophoresis

et al. 2021

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“…There have been various reports about glioblastoma cell culture isolation, each with a different technique, and advantages and drawbacks (30)(31)(32)(33). We have developed a simplified protocol for enriched primary glioblastoma culture, derived from neurosurgical patients.…”

Section: Discussionmentioning

confidence: 99%

The Isolation of Human Glioblastoma Cells: An Optimised Protocol

Velnar

Maver

Bošnjak

et al. 2021

ama

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Objective. The aim of this study was to establish an optimised protocol for glioblastoma (GBM) cell isolation from brain resection samples, with a high yield and low risk for contamination.Methods. Human GBM cells can be obtained following cranial tumour operations. In sterile conditions, the fragments of viable tissue removed during surgery were collected. The tissue was cut and mechanically coarsely decomposed. The sediment was harvested after centrifugation, the cells were seeded in suspension, and supplemented with a special medium (Advanced DMEM) containing high level nutrients and antibiotics.Results. In an appropriate environment, the isolated cells retained viability and proliferated quickly. Attachments were observed after ten hours, and proliferation after two days. The time to full confluence was about one week. The cells were stable. Under standard culture conditions, cell proliferation and cluster formation were observed. Cell viability was 95%.Conclusion. The protocol described for isolation is easy, quick and affordable, leading to stable GBM cells. The isolation technique provides sufficient quantities of isolated cells that may be used as an important new tool for in vitro research. The availability of this system will permit the study of cell properties, biochemical aspects, and provides the potential of therapeutic candidates for pathological disorders in a well-controlled environment.

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The feasibility of using dielectrophoresis for isolation of glioblastoma subpopulations with increased stemness

Cited by 14 publications

References 39 publications

Dielectrophoresis: Developments and applications from 2010 to 2020

Dielectrophoresis: Developments and applications from 2010 to 2020

Automated fluorescence quantification of extracellular vesicles collected from blood plasma using dielectrophoresis

The Isolation of Human Glioblastoma Cells: An Optimised Protocol

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