The first reported case of Burkholderia contaminans in patients with cystic fibrosis in Ireland: from the Sargasso Sea to Irish Children

Power, Rachel F.; Linnane, Barry; Martin, R.; Power, Noelle; Harnett, Peig; Casserly, Brian; O’Connell, Nuala H.; Dunne, Colum P.

doi:10.1186/s12890-016-0219-z

Cited by 19 publications

(11 citation statements)

References 29 publications

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“…Taxon K now consists of two validly named species, Burkholderia contaminans and Burkholderia lata , and has been isolated from numerous human and environmental sources, including CF sputum, blood, cerebrospinal fluid, river water, sediment, soil, and various plants ( Baldwin et al, 2005 ; Mahenthiralingam et al, 2006 ; Cesarini et al, 2009 ; da Silva et al, 2012 ). B. contaminans can be considered an emerging CF pathogen; it is the most common Bcc species in CF patients in Argentina ( Cipolla et al, 2018 ) and it is increasingly isolated in CF centers in Spain ( Medina-Pascual et al, 2015 ), the United Kingdom ( Kenna et al, 2017 ), Portugal ( Coutinho et al, 2015 ), and Ireland ( Power et al, 2016 ). In addition, these organisms are frequently responsible for outbreaks of healthcare-associated infections due to contamination of pharmaceutical products such as nasal spray ( CDC, 2004 ), dialysis water ( Souza et al, 2004 ), moist washcloths ( Martin et al, 2011 ), mouthwash ( Zurita et al, 2014 ), and liquid docusate laxative ( Glowicz et al, 2018 ).…”

Section: Introductionmentioning

confidence: 99%

Burkholderia cepacia Complex Taxon K: Where to Split?

et al. 2020

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The objective of the present study was to provide an updated classification for Burkholderia cepacia complex (Bcc) taxon K isolates. A representative set of 39 taxon K isolates were analyzed through multilocus sequence typing (MLST) and phylogenomic analyses. MLST analysis revealed the presence of at least six clusters of sequence types (STs) within taxon K, two of which contain the type strains of Burkholderia contaminans (ST-102) and Burkholderia lata (ST-101), and four corresponding to the previously defined taxa Other Bcc groups C, G, H and M. This clustering was largely supported by a phylogenomic tree which revealed three main clades. Isolates of B. contaminans and of Other Bcc groups C, G, and H represented a first clade which generally shared average nucleotide identity (ANI) and average digital DNA-DNA hybridization (dDDH) values at or above the 95-96% ANI and 70% dDDH thresholds for species delineation. A second clade consisted of Other Bcc group M bacteria and of four B. lata isolates and was supported by average ANI and dDDH values of 97.2 and 76.1% within this clade and average ANI and dDDH values of 94.5 and 57.2% toward the remaining B. lata isolates (including the type strain), which represented a third clade. We therefore concluded that isolates known as Other Bcc groups C, G, and H should be classified as B. contaminans, and propose a novel species, Burkholderia aenigmatica sp. nov., to accommodate Other Bcc M and B. lata ST-98, ST-103, and ST-119 isolates. Optimized MALDI-TOF MS databases for the identification of clinical Burkholderia isolates may provide correct species-level identification for some of these bacteria but would identify most of them as B. cepacia complex. MLST facilitates species-level identification of many taxon K strains but some may require comparative genomics for accurate species-level assignment. Finally, the inclusion of Other Bcc groups C, G, and H into B. contaminans affects the phenotype of this species minimally and the proposal to classify Other Bcc group M and B. lata ST-98, ST-103, and ST-119 strains as a novel Burkholderia species is supported by a distinctive phenotype, i.e., growth at 42 • C and lysine decarboxylase activity.

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Section: Introductionmentioning

confidence: 99%

Burkholderia cepacia Complex Taxon K: Where to Split?

et al. 2020

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“…contaminans do not lose the OAg even during long term infections that last for periods of time as extended as 9.6 and 15.2 years, respectively. Among the less frequent species, B. contaminans is currently considered an emerging CF pathogen (Vanlaere et al, 2009; Martina et al, 2013; Coutinho et al, 2015; Medina-Pascual et al, 2015; Nunvar et al, 2016; Power et al, 2016). Concerning the other two rarely found species examined, B .…”

Section: Discussionmentioning

confidence: 99%

Burkholderia cepacia Complex Species Differ in the Frequency of Variation of the Lipopolysaccharide O-Antigen Expression During Cystic Fibrosis Chronic Respiratory Infection

Hassan

Coutinho

Sá‐Correia

2019

Front. Cell. Infect. Microbiol.

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Burkholderia cepacia complex (Bcc) bacteria can adapt to the lung environment of cystic fibrosis (CF) patients resulting in the emergence of a very difficult to eradicate heterogeneous population leading to chronic infections associated with rapid lung function loss and increased mortality. Among the important phenotypic modifications is the variation of the lipopolysaccharide (LPS) structure at level of the O-antigen (OAg) presence, influencing adherence, colonization and the ability to evade the host defense mechanisms. The present study was performed to understand whether the loss of OAg expression during CF infection can be considered a general phenomenon in different Bcc species favoring its chronicity. In fact, it is still not clear why different Bcc species/strains differ in their ability to persist in the CF lung and pathogenic potential. The systematic two-decade-retrospective-longitudinal-screening conducted covered 357 isolates retrieved from 19 chronically infected patients receiving care at a central hospital in Lisbon. The study involved 21 Bcc strains of six/seven Bcc species/lineages, frequently or rarely isolated from CF patients worldwide. Different strains/clonal variants obtained during infection gave rise to characteristic OAg-banding patterns. The two most prevalent and feared species, B. cenocepacia and B. multivorans , showed a tendency to lose the OAg along chronic infection. B . cenocepacia recA lineage IIIA strains known to lead to particularly destructive infections exhibit the most frequent OAg loss, compared with lineage IIIB. The switch frequency increased with the duration of infection and the level of lung function deterioration. For the first time, it is shown that the rarely found B. cepacia and B. contaminans , whose representation in the cohort of patients examined is abnormally high, keep the OAg even during 10- or 15-year infections. Data from co-infections with different Bcc species reinforced these conclusions. Concerning the two other rarely found species examined, B . stabilis exhibited a stable OAg expression phenotype over the infection period while for the single clone of the more distantly related B . dolosa species, the OAg-chain was absent from the beginning of the 5.5-year infection until the patient dead. This work reinforces the relevance attributed to the OAg-expression switch suggesting marked differences in the various Bcc species.

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“…B. contaminans is prevalent in Argentina (7) and Portugal (8) and is being increasingly isolated in CF patients from Spain (9) and Ireland (10). B. contaminans has been linked to outbreaks due to contaminated pharmaceutical products (11, 12).…”

Section: Genome Announcementmentioning

confidence: 99%