The flagella of F18ab Escherichia coli is a virulence factor that contributes to infection in a IPEC-J2 cell model in vitro

Duan, Qiangde; Zhu, Guoqiang; Zhu, Xiaofang; Bao, Wenbin; Wu, Shenglong; Ruan, Xiaosai; Zhang, Weiping; Yang, Yang; Zhu, Jun; Zhu, Guoqiang

doi:10.1016/j.vetmic.2012.05.015

Cited by 54 publications

(37 citation statements)

References 31 publications

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“…Defects in a host's defense mechanisms, e.g., urinary or biliary obstruction, can result in the persistence of E. coli isolates within the urinary and biliary tract and induce serious or recurrent infections. E. coli isolates from UTI and BTI exhibiting lower motility but more virulence genes in this study suggests a different role [7,13,23,24]. In E. coli and Pseudomonas aeruginosa, flagellin and/or the distally located flagellar cap protein have been reported to function as adhesins [25].…”

Section: Discussionmentioning

confidence: 68%

The complex interplay among bacterial motility and virulence factors in different Escherichia coli infections

Kao

Lin

Tseng

et al. 2014

Eur J Clin Microbiol Infect Dis

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Motility mediated by the flagella of Escherichia coli is important for the bacteria to move toward host cells. Here, we present the relationship among bacterial motility, virulence factors, antimicrobial susceptibility, and types of infection. A total of 231 clinical E. coli isolates from different infections were collected and analyzed. Higher-motility strains (motility diameter ≥6.6 mm) were more common in spontaneous bacterial peritonitis (SBP) (SBP 59 %, colonization 32 %, urinary tract infection 16 %, urosepsis 34 %, and biliary tract infection 29 %; p < 0.0001). Compared with the higher-motility group, there was a higher prevalence of afa and ompT genes (p = 0.0160 and p = 0.0497, respectively) in E. coli strains with lower motility. E. coli isolates with higher and lower motility were in different phylogenetic groups (p = 0.018), with a lower prevalence of A and B1 subgroups in higher-motility strains. Also, the patterns of virulence factors and antibiotic susceptibility of E. coli isolates derived from various infections were significantly different. This study demonstrates that the prevalence of higher-motility strains was greater in E. coli isolates from SBP compared to other types of infection. Various types of E. coli infection were associated with differences in bacterial motility, virulence factors, and antibiotic susceptibility. More bacterial virulence factors may be necessary for the development of extraintestinal infections caused by E. coli isolates with lower motility.

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Section: Discussionmentioning

confidence: 68%

The complex interplay among bacterial motility and virulence factors in different Escherichia coli infections

Kao

Lin

Tseng

et al. 2014

Eur J Clin Microbiol Infect Dis

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“…Strains were seeded into biofilm-inducing medium in either glass test tubes or in 96-well plates as described previously (Pratt and Kolter 1998;Li et al 2008;Duan et al 2012b). OD 600 values of each well were read to measure the amount of biofilm through attached crystal violet.…”

Section: Crystal Violet Methods For Quantification Of Biofilm Formationmentioning

confidence: 99%

“…After Triton X-110 treatment, the number of bacteria that invaded IPEC-J2 cells was enumerated (Duan et al 2012b(Duan et al , 2013.…”

Section: Bacterial Invasion Assaysmentioning

confidence: 99%

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Quorum-sensing gene luxS regulates flagella expression and Shiga-like toxin production in F18ab Escherichia coli

et al. 2014

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To investigate the effect of the luxS gene on the expression of virulence factors in Shiga-like toxin producing and verotoxin-producing Escherichia coli, the luxS gene from E. coli 107/86 (wild type, O139:H1:F18ab, Stx2e) was deleted. The successful deletion of luxS was confirmed by bioluminescence assays. The luxS deletion mutant exhibited changed flagella-related phenotypes, like impaired expression of flagella, decreased flagella motility, reduced biofilm formation, and reduced ability to induce pro-immunity response in host cells, which were restored after complementation with the intact luxS gene. The mutant strain also displayed attenuated production of Stx2e. This study provides new information to the crucial function of luxS in regulating Shiga-like toxin producing E. coli virulence.

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“…The flagella of ETEC and Shiga-like toxin-producing E. coli (SLTEC) have accessory functions as both adhesins and invasins (Duan et al 2013a, b) and contribute to biofilm formation (Duan et al 2012;Zhou et al 2014). Flagellin, the major subunit of this bacterial surface organelle, plays an important role in mediating bacterial interactions with the host cell membrane.…”

Section: Introductionmentioning

confidence: 99%

Membrane cholesterol plays an important role in enteropathogen adhesion and the activation of innate immunity via flagellin–TLR5 signaling

Zhu

Duan

et al. 2015

Arch Microbiol

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Lipid rafts are cholesterol- and sphingolipid-rich ordered microdomains distributed in the plasma membrane that participates in mammalian signal transduction pathways. To determine the role of lipid rafts in mediating interactions between enteropathogens and intestinal epithelial cells, membrane cholesterol was depleted from Caco-2 and IPEC-J2 cells using methyl-β-cyclodextrin. Cholesterol depletion significantly reduced Escherichia coli and Salmonella enteritidis adhesion and invasion into intestinal epithelial cells. Complementation with exogenous cholesterol restored bacterial adhesion to basal levels. We also evaluated the role of lipid rafts in the activation of Toll-like receptor 5 signaling by bacterial flagellin. Depleting membrane cholesterol reduced the ability of purified recombinant E. coli flagellin to activate TLR5 signaling in intestinal cells. These data suggest that both membrane cholesterol and lipid rafts play important roles in enteropathogen adhesion and contribute to the activation of innate immunity via flagellin-TLR5 signaling.

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The flagella of F18ab Escherichia coli is a virulence factor that contributes to infection in a IPEC-J2 cell model in vitro

Cited by 54 publications

References 31 publications

The complex interplay among bacterial motility and virulence factors in different Escherichia coli infections

The complex interplay among bacterial motility and virulence factors in different Escherichia coli infections

Quorum-sensing gene luxS regulates flagella expression and Shiga-like toxin production in F18ab Escherichia coli

Membrane cholesterol plays an important role in enteropathogen adhesion and the activation of innate immunity via flagellin–TLR5 signaling

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