The FORMATION OF CHONDROMUCOPROTEIN-FIBRINOGEN AND CHONDROMUCOPROTEIN-β-LIPOPROTEIN COMPLEXES

Aj, Anderson

doi:10.1042/bj0880460

Cited by 45 publications

(10 citation statements)

References 39 publications

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“…It is somewhat easier to inter pret a rise in any one of the sulphated fractions, since it is known that they bind fibrinogen, calcium, low-density lipoproteins and interact with platelets (1,7,8,17,18). DS in particular is known to have a strong affinity for binding very low and low density lipoproteins (8).…”

Section: Discussionmentioning

confidence: 99%

Arterial Glycosaminoglycans in Diabetic Dogs

Sirek¹,

Sirek²,

Cukerman³

1980

J Vasc Res

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The glycosaminoglycan (GAG) composition of a number of large and medium-sized arteries was studied in 6 alloxan-diabetic beagles and was compared with 6 normal, age-matched controls. Diabetic animals were maintained on diet and insulin for 100 days. The aortic arch, thoracic and abdominal segments, external iliac, superior mesenteric, renal, common carotid and coronary arteries were analyzed for hyaluronic acid (HA) and for heparan (HS), dermatan (DS), and chondroitin (CS) sulphates. All diabetic dogs displayed significant alterations. The HA content was reduced in iliac arteries, and together with HS, also in the thoracic aorta. HS or CS were increased in carotid, iliac and renal arteries, DS, a GAG constituent with very high affinity for low density lipoproteins, was significantly increased in coronary arteries alone. 2 additional animals which are excluded from this series did not become diabetic after alloxanization and showed no change in arterial GAG content. Early changes in the chemistry of the arterial ground substance seem to provide a clue to the precocious development of atherosclerotic disease in diabetes.

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Section: Discussionmentioning

confidence: 99%

Arterial Glycosaminoglycans in Diabetic Dogs

Sirek¹,

Sirek²,

Cukerman³

1980

J Vasc Res

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“…The poor precipitation of mucin complexes with fresh serum might alternatively be explained by selective bonding between hyaluronic acid and labile serum proteins with properties favouring formation of stable colloidal micelles. Many possible combinations of acid mucopolysaccharides and proteins, from serum and other sources, can precipitate like mucin complexes in appropriate conditions (Ogston, personal communication; see also Anderson, 1963), but soluble protein: polysaccharide complexes, other than those with pre-existing covalent linkages, are much more difficult to demonstrate (Noguchi, 1956). However, in conditions of physiological pH, hyaluronic acid forms soluble complexes in normal bovine synovial fluid (Ogston and Stanier, 1950) with acid-insoluble a-globulins (Curtain, 1955) and in abnormal human synovial fluid with serum inter-a trypsin inhibitor (Sandson, Hamerman, and Schwick 1965).…”

Section: Discussionmentioning

confidence: 99%

Modification of mucin clots by serum.

Fraser¹,

Harris²

1971

Annals of the Rheumatic Diseases

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“…While hyaluronic acid is chiefly concerned with hydration of tissues [19], sulphated GAG are known to bind low-density lipoproteins, fibrinogen and calcium, and they also interact with platelets [14,20,21,22]. Dermatan sulphate in particular is known to have a strong affinity for binding very low-and lowdensity lipoproteins [14].…”

Section: Discussionmentioning

confidence: 99%

Intermittent hyperinsulinaemia and arterial glycosaminoglycans in dogs

Sirek

Cukerman

1981

Diabetologia

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Summary. The effect of insulin on the glycosaminoglycan content of the arterial ground substance was compared in age-matched alloxan-diabetic dogs, hypophysectomized dogs and normal controls. Hyaluronic acid and the three sulphated components, heparan, dermatan and isomeric chondroitin sulphates were quantitatively determined in the arch, thoracic and abdominal aorta, and in the carotid, coronary, iliac, renal and mesenteric arteries. Diabetic animals were either well or poorly controlled. Six well controlled dogs with a fasting mean plasma glucose level of 8.5 mmol/1 were given around 30 units of porcine insulin/day and showed fluctuations in plasma insulin concentrations between 24 and 175 mU/1. Four poorly controlled dogs with a mean fasting plasma glucose level of 18.5 mmol/1 received an average of 14 units of insulin/day, and the fluctuations ranged from 8 to 113 mU/1. Eight normal, untreated controls showed mean fluctuations between 15 and 22 mU/1. Within 14 weeks of insulin treatment, well controlled animals displayed glycosaminoglycan alterations in five arterial segments, usually involving more than one glycosaminoglycan constituent. In poorly controlled animals the number of segments that showed glycosaminoglycan alterations was the same, but abnormalities were limited to one of the sulphated components only. The coronary arteries displayed identical glycosaminoglycan alterations in the two groups of diabetic dogs, namely a significant rise in dermatan sulphate content (p < 0.01, mean _+ SEM) from the normal value of 1.12 _+ 0.03 mg/g dry defatted tissue to 1.31 + 0.03 mg/g in well controlled animals and to 1.37 _ 0.08 mg/g in poorly controlled animals. In order to ascertain that the abnormalities in the glycosaminoglycan chemistry were related to hyperinsulinaemia rather than hyperglycaemia, six hypophysectomized dogs were treated with 1.5 U/day of porcine protamine zinc insulin, for 3 weeks. Average plasma glucose levels were in the order of 4.3 mmol/1 and plasma insulin levels fluctuated between 9 and 27 mU/1; the latter represented twice the peak value seen in five untreated hypophysectomized dogs. The resulting chemical changes in the glycosaminoglycan content were in line with those encountered in diabetic animals, including the rise in dermatan sulphate content of the coronary arteries. These results indicate that: (1) hyperinsulinaemia produced by injections of insulin causes alterations of the arterial glycosaminoglycan content; (2) not all segments of the arterial tree are equally responsive to insulin and (3) the coronary arteries have a particularly insulin-sensitive dermatan sulphate metabolism.Key words: Diabetic and hypophysectomized dogs, insulin, arterial glycosaminoglycans.There appears to be a concensus of opinion that good management of diabetes requires more than normalization of blood glucose levels if coronary heart disease and late vascular complications in general are to be prevented [1,2]. The artificial B cell has focused attention on physiological 24 h plasma insulin profile...

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The FORMATION OF CHONDROMUCOPROTEIN-FIBRINOGEN AND CHONDROMUCOPROTEIN-β-LIPOPROTEIN COMPLEXES

Cited by 45 publications

References 39 publications

Arterial Glycosaminoglycans in Diabetic Dogs

Arterial Glycosaminoglycans in Diabetic Dogs

Modification of mucin clots by serum.

Intermittent hyperinsulinaemia and arterial glycosaminoglycans in dogs

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