The function <i>in vitro</i> of macrophages from the intestinal mucosa of patients with Crohn's disease: An association between chemotactic migration and granulomata

Hermanowicz, A; Pallone, Francesco; Mason, C H

doi:10.1111/j.1440-1746.1988.tb00226.x

Cited by 6 publications

(5 citation statements)

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“…The proportion of IL-2R bearing lamina propria mononuclear cells in Crohn's disease was significantly higher than that of autologous peripheral blood mononuclear cells and that of control lamina propria and peripheral blood cells (21 (6)%, 6 (2)%, 9 (4)%, and 1 (0.5)% respectively, p<00 1).…”

Section: Resultsmentioning

confidence: 81%

Spontaneous release of interferon gamma by intestinal lamina propria lymphocytes in Crohn's disease. Kinetics of in vitro response to interferon gamma inducers.

Fais

Capobianchi

Pallone

et al. 1991

Gut

161

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Section: Resultsmentioning

confidence: 81%

Spontaneous release of interferon gamma by intestinal lamina propria lymphocytes in Crohn's disease. Kinetics of in vitro response to interferon gamma inducers.

Fais

Capobianchi

Pallone

et al. 1991

Gut

161

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“…A mucosal-specific depression of the chemotaxis of intestinal macrophages was demonstrated recently in patients with nongranulomatous Crohn's disease. 73 Macrophages from tissue containing granulomata exhibited normal chemotaxis. This study does not indicate why granulomata form in Crohn's disease but may give clues to why they do not form in some patients.…”

Section: Abnormal Inflammatory Responses To Luminal Antigensmentioning

confidence: 99%

Current concepts in the pathogenesis of Crohn's disease

GIBSON

1990

J of Gastro and Hepatol

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“…I 13] For macrophages to respond to antigenic stimuli, they must first be primed by specific signals such as interferon (lFN)-y released by T cells. [74][75][76] Studies of isolated macrophages from inflamed intestine have to date not revealed any functional deficiencies, although their chemotaxis in vitro was depressed in patients with granulomas.l 77 ] However, the heterogeneity of the populations studied (especially with regard to resident versus recently arrived 'inflammatory' macrophages) do not permit a definitive conclusion that macrophage dysfunction is not present. Unprimed macrophages respond poorly to triggering signals.…”

Section: Antigen Handling By Macrophagesmentioning

confidence: 99%

Inflammatory Bowel Disease

Gibson

1994

Clin. Immunother.

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In the US, H. pylori vacA shows allelic variation in the signal sequence (which may be type sla, slb or s2) and the mid-region (type ml or m2). Previous PCR-based vacA mid-region typing classified most, but not all, Asian and South American strains tested as ml or m2. We now sought to investigate vacA mid-region diversity further.MotlmodL We studied 13 Japanese, 6 Chinese, 9 Thai, and 8 Peruvian H. pylori isolates. cagA was identified by colony hybridisation (CH), vacA signal sequence was typed by PCR, and vacA mid-region was typed proximally by CH and distally by PCR. Sections of the vacA mid-region from 8 strains were PCR-amplified, sequenced, and compared with known sequences from 8 other strains.Rcuts Of the 36 Asian and South American strains studied, 35 were cagA+ (the cagA-was Peruvian) and 35 were vacA sla (1 cagA + Peruvian was slb). vacA mid-regions from the 13 Japanese strains were not PCR-amplified by ml or m2-specific primers, but hybridised weakly with an ml probe. Sequence analysis of vacA from 1 Japanese strain revealed 91% nucleotide identity with the ml probe but only 71% identity with the m2 probe. The previously equivocal Thai and Peruvian strains also had ml-like mid-region sequences. A Chinese strain was ml in the proximal mid-region and m2 distally, showing a clear crossover site. Final mid-region types were: Japanese, all 13 ml; Chinese, 1 ml, 1 ml/m2, 4 m2; Thai, 3 ml, 6 m2; Peruvian 4 ml, 4 m2. Distal mid-region sequences of 16 strains, compared over 294 bp, clustered into 2 groups, ml and m2. Nucleotide identity between ml and m2 strains ranged from 73-78%. Within groups, m2 strains were less diverse than ml strains (m2 range 94-99.7%, ml 88-99.3%, p<0.001). Sequence analysis of 7 ml and 3 m2 strains over 1.1kb proximally showed maintenance of clustering outside the 294bp region C duions These Asian and South American strains are similar in terms of cagA status and vacA sla genotype, but fall into 2 vacA midregion groups. ml sequences are more diverse than m2, and thus may be phylogenetically older. The vacA sequence of 1 Chinese strain suggests recombination in vivo between ml and m2 alleles. Introduction: H.pylori antimicrobial susceptibility is an important determinant ofthe efficacy of eradication therapies'. The prevalence of antimicrobial resistance varies within the UK and may increase given the increased use of eradication therapy. This multicentre study assesses the prevalence and possible associations ofH.pylon antimicrobial resistance. Methods: H.pylon was isolated from antral biopsies of patients undergoing routine endoscopy and cultured according to standard microbiological methods. Antimicrobial resistance was determined using "E-tests" or disc tests (tinidazole only) with breakpoints defined by previous studies. Results: H.pyloni was isolated from 32% (1222/3823) of patients and antimicrobial susceptibility determined in 90% (1077/1222) Previous case-control studies of IBD have used hospital or community derived controls. There are obvious inherent biases in both metho...

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The function in vitro of macrophages from the intestinal mucosa of patients with Crohn's disease: An association between chemotactic migration and granulomata

Cited by 6 publications

References 20 publications

Spontaneous release of interferon gamma by intestinal lamina propria lymphocytes in Crohn's disease. Kinetics of in vitro response to interferon gamma inducers.

Spontaneous release of interferon gamma by intestinal lamina propria lymphocytes in Crohn's disease. Kinetics of in vitro response to interferon gamma inducers.

Current concepts in the pathogenesis of Crohn's disease

Inflammatory Bowel Disease

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