The function of a hydrogen peroxide-detecting electroenzymatic glucose electrode is markedly impaired in human sub-cutaneous tissue and plasma

Kerner, W.; Kiwit, Martin; Linke, Burkhard; Keck, F. S.; Zier, H; Pfeiffer, E. F.

doi:10.1016/0956-5663(93)80032-k

Cited by 74 publications

(45 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Kerner 16 first discovered that LMWM of <10 kDa (e.g., ascorbate, urate, cysteine, reduced glutathione and alphatocopherol, tyrosine, and tryptophan) could lead to the rapid degradation of GOx and a dramatically lowered sensitivity. Pinpointing the cause of LMWM degradation of GOx is difficult due to numerous unknown events that occur in the surrounding tissue and within the sensor itself that can lead to gradual failure.…”

Section: Low Molecular Weight Materials Degradation Of Glucose Oxidasementioning

confidence: 99%

“…Suspected causes of GOx degradation include hydrogen peroxide (H 2 O 2 ) generated at the electrode [10][11][12][13][14][15] and intrinsic LMWM from blood and interstitial fluid. 16 However, eliminating these factors is not enough to ensure optimum GOx performance; in addition, we must engineer a more stable environment for GOx and make the GOx enzyme itself more intrinsically stable.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Common Causes of Glucose Oxidase Instability in In Vivo Biosensing: A Brief Review

Harris

Reyes

López

2013

J Diabetes Sci Technol

View full text Add to dashboard Cite

Abbreviations: (CBD) chitin-binding domain, (ELP) elastin-like polypeptide, (GAX) glutaraldehyde cross-linking, (GOx) AbstractClinical management of diabetes must overcome the challenge of in vivo glucose sensors exhibiting lifetimes of only a few days. Limited sensor life originates from compromised enzyme stability of the sensing enzyme. Sensing enzymes degrade in the presence of low molecular weight materials (LMWM) and hydrogen peroxide in vivo. Sensing enzymes could be made to withstand these degradative effects by (1) stabilizing the microenvironment surrounding the sensing enzyme or (2) improving the structural stability of the sensing enzyme genetically. We review the degradative effect of LMWM and hydrogen peroxide on the sensing enzyme glucose oxidase (GOx). In addition, we examine advances in stabilizing GOx against degradation using hybrid silica gels and genetic engineering of GOx. We conclude molecularly engineered GOx combined with silica-based encapsulation provides an avenue for designing long-term in vivo sensor systems.

show abstract

Section: Low Molecular Weight Materials Degradation Of Glucose Oxidasementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Common Causes of Glucose Oxidase Instability in In Vivo Biosensing: A Brief Review

Harris

Reyes

López

2013

J Diabetes Sci Technol

View full text Add to dashboard Cite

show abstract

“…After years of research, progress has finally being made toward inplantable continuous glucose monitors (Pfeiffer et al, 1995;Hurd et al, 1988;Kerner et al, 1993). Two different kinds of devices have been proposed: fully implantable and percutaneous (worn through the skin).…”

Section: Introductionmentioning

confidence: 99%

A microdialysis technique for continuous subcutaneous glucose monitoring in diabetic patients (part 1)

Poscia

Mascini

Moscone

et al. 2003

Biosensors and Bioelectronics

View full text Add to dashboard Cite

The performances and the stability of a novel subcutaneous glucose monitoring system have been evaluated. GlucoDay † (A. Menarini I.F.R. S.r.l, Florence Italy) is a portable instrument provided with a micro-pump and a biosensor coupled to a microdialysis system capable of recording the subcutaneous glucose level every 3 min. Long and short term stability of the biosensor are discussed and the results of some critical in vitro and in vivo (on rabbits) experiments are reported. A linear response up to 30 mM has been found for in vivo glucose concentration. The sensitivity referred to blood glucose is better than 0.1 mM and the zero current is typically below the equivalent of 0.1 mM. In the accuracy study a mean bias of 2.7 mg/dl and a correlation coefficient equal to 0.9697 have been found. At room temperature, an excellent membrane stability assures good performances up to 6 months from the first use. #

show abstract

“…The external component of the current passes from the anode, through the membranes and tissue or blood, to a nearby cathode. The main difficulties associated with this type of sensor include electrochemical interference [15,16], accounting for local oxygen variability [12,13], and peroxide-mediated enzyme inactivation [17].…”

Section: Physical Principles For Continuous Sensorsmentioning

confidence: 99%

Advances and prospects in glucose assay technology

1997

View full text Add to dashboard Cite

Glucose assay has long been central to therapy of diabetes mellitus. In the spirit of this occasion commemorating the 75th Anniversary of the Discovery of Insulin, we review here some of the important advances in glucose assay methodology, give our opinions on the present status of glucose assay technologies, and speculate on potential advances in the near future. Some aspects of this review have recently been published elsewhere [1]. A brief historyGlucose assays have been central to the treatment of diabetes from the early days of treatment. The discovery of the effect of insulin at the biochemical level was made possible by an early glucose assay based on the chromogenic reduction of a copper solution by glucose [2]. This assay and its successive modifications were not completely specific for glucose in the presence of other reducing sugars and required careful preparation of relatively large samples, but were nevertheless useful for laboratory assay of glucose in blood and urine. In the following years, clinical glucose assay methodology evolved rapidly, incorporating enzymes for specificity, requiring less sample preparation, smaller volumes, and becoming fully automated [2].Qualitative tests for glucose were later developed based on glucose oxidase and reagents impregnated in paper strips. This advance facilitated patient involvement in glucose assay and the approach was used widely for urine glucose determinations because of its convenience, even though the assay was originally considered less reliable than blood assays. In the past decade, this technology has advanced dramatically in conjunction with the development of home glucometer instrumentation, to the point of providing quantitative, convenient and inexpensive blood glucose determination. The evolution of this technology represents a revolutionary improvement in the management of diabetes, as widespread, frequent and quantitative blood glucose testing is now possible, leading to more appropriate insulin administration schedules and closer involvement of the patient in therapy [3]. Desired new featuresThe results of the Diabetes Control and Complications Trial [4] suggest the value of close blood glucose control in reducing the complications of Diabetologia (1997) Summary The evolution of glucose assay methods has been central to the development of present therapies for diabetes mellitus. However, new blood glucose assay capabilities, such as convenient and truly continuous monitoring, are now needed to foster a new era of close blood glucose control. The principles and present status of glucose sensors being developed that may fill these requirements are reviewed here.

show abstract

The function of a hydrogen peroxide-detecting electroenzymatic glucose electrode is markedly impaired in human sub-cutaneous tissue and plasma

Cited by 74 publications

References 31 publications

Common Causes of Glucose Oxidase Instability in In Vivo Biosensing: A Brief Review

Common Causes of Glucose Oxidase Instability in In Vivo Biosensing: A Brief Review

A microdialysis technique for continuous subcutaneous glucose monitoring in diabetic patients (part 1)

Advances and prospects in glucose assay technology

Contact Info

Product

Resources

About