The Function of MoGlk1 in Integration of Glucose and Ammonium Utilization in Magnaporthe oryzae

Zhang, Lisha; Lv, Ruili; Dou, Xiaowei; Qi, Zhongqiang; Hua, Chenlei; Zhang, Haifeng; Wang, Zhengyi; Zheng, Xiaobo

doi:10.1371/journal.pone.0022809

Cited by 16 publications

(13 citation statements)

References 57 publications

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“…Conidia were harvested with double-distilled water and filtered through with one layer Miracloth (Calbiochem, San Diego, CA, USA). Conidial germination and appressorial formation were observed on Gel Bond film (FMC Bioproducts, Rockland, ME, USA) as described previously (Zhang et al, 2011c;Chen et al, 2014). At least 100 conidia per replicate were examined.…”

Section: Analysis Of Conidial Morphology Conidial Germination Apprementioning

confidence: 99%

Threonine deaminase MoIlv1 is important for conidiogenesis and pathogenesis in the rice blast fungus Magnaporthe oryzae

Tang

et al. 2014

Fungal Genetics and Biology

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Section: Analysis Of Conidial Morphology Conidial Germination Apprementioning

confidence: 99%

Threonine deaminase MoIlv1 is important for conidiogenesis and pathogenesis in the rice blast fungus Magnaporthe oryzae

Tang

et al. 2014

Fungal Genetics and Biology

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“…Mycelia were collected from 2-day-old liquid culture for the measurement of biomass, and then washed by distilled water and lyophilized. Fungal biomass was determined by mycelial dry weight (Zhang et al, 2011c). All the experiments were repeated three times, each with three replicates.…”

Section: Phenotype Assaysmentioning

confidence: 99%

Acetolactate synthases MoIlv2 and MoIlv6 are required for infection‐related morphogenesis in Magnaporthe oryzae

Zhang

et al. 2013

Molecular Plant Pathology

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Amino acids are important components in the metabolism of a variety of pathogens, plants and animals. Acetolactate synthase (ALS) catalyses the first common step in leucine, isoleucine and valine biosynthesis, and is the target of several classes of inhibitors. Here, MoIlv2, an orthologue of the Saccharomyces cerevisiae ALS catalytic subunit Ilv2, and MoIlv6, an orthologue of the S. cerevisiae ALS regulatory subunit Ilv6, were identified. To characterize MoILV2 and MoILV6 functions, we generated the deletion mutants ΔMoilv2 and ΔMoilv6. Phenotypic analysis showed that both mutants were auxotrophic for leucine, isoleucine and valine, and were defective in conidial morphogenesis, appressorial penetration and pathogenicity. Further studies suggested that MoIlv2 and MoIlv6 play a critical role in maintaining the balance of intracellular amino acid levels. MoIlv2 and MoIlv6 are both localized to the mitochondria and the signal peptide of MoIlv6 is critical for its localization. In summary, our evidence indicates that MoIlv2 plays a crucial role in isoleucine and valine biosynthesis, whereas MoIlv6 contributes to isoleucine and leucine biosynthesis; both genes are required for fungal pathogenicity. This study indicates the potential of targeting branched-chain amino acid biosynthesis for anti-rice blast management.

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“…However, the Δmohex1 mutant was indistinguishable from the wild‐type in its ability to catabolize GlcNAc. This may be due to the functional redundancy as several hexokinases are known to be present in M. oryzae (Zhang et al ., ) (http://onlinelibrary.wiley.com/doi/10.1111/1462-2920.13215/suppinfo).…”

Section: Resultsmentioning

confidence: 99%

Magnaporthe oryzae aminosugar metabolism is essential for successful host colonization

Kumar

Ghosh

Bhatt

et al. 2016

Environmental Microbiology

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Pathogens encounter and metabolize a range of host-derived metabolites while proliferating inside the host. Our understanding of these metabolites and their metabolic processes has remained largely incomplete. We investigated the role of the Magnaporthe oryzae N-acetylglucosamine (GlcNAc) catabolic pathway during rice infection. The catabolic pathway is composed of a GlcNAc transporter (MoNgt1), hexokinase(s), a GlcNAc-6-phosphate deacetylase (MoDac) and a GlcN-6-phosphate deaminase (MoDeam). A detailed characterization of the Δmongt1, Δmodac and Δmodeam null mutants revealed that a defect in GlcNAc catabolism impairs the pathogenicity of M. oryzae. These mutants showed severely reduced virulence in susceptible rice cultivar due to their inability to neutralize host-derived reactive oxygen species and their failure to develop invasive hyphal growth within the host tissue. Interestingly, during oxidative stress, M. oryzae proliferated efficiently in GlcNAc-containing media compared with other sugars, and the expression of fungal antioxidant genes was upregulated following GlcNAc treatment. However, GlcNAc inhibited the growth of the Δmodac and Δmodeam mutants, and this growth inhibition was enhanced during oxidative stress. These results suggest that GlcNAc helps fungus to overcome oxidative stress inside its host, perhaps by activating an antioxidant defence. In the absence of a functional catabolic pathway, GlcNAc becomes toxic to the cells.

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The Function of MoGlk1 in Integration of Glucose and Ammonium Utilization in Magnaporthe oryzae

Cited by 16 publications

References 57 publications

Threonine deaminase MoIlv1 is important for conidiogenesis and pathogenesis in the rice blast fungus Magnaporthe oryzae

Threonine deaminase MoIlv1 is important for conidiogenesis and pathogenesis in the rice blast fungus Magnaporthe oryzae

Acetolactate synthases MoIlv2 and MoIlv6 are required for infection‐related morphogenesis in Magnaporthe oryzae

Magnaporthe oryzae aminosugar metabolism is essential for successful host colonization

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