The Future of Cell Therapy and Tissue Engineering in Cardiovascular Disease: The New Era of Biological Therapeutics

Heydarkhan‐Hagvall, Sepideh; Nsair, Ali; Beygui, Ramin E.; Shemin, Richard J.

doi:10.5772/8589

Cited by 1 publication

(2 citation statements)

References 108 publications

(154 reference statements)

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“…Tissue engineering (TE) is an emerging biomedical discipline which aims to create artificially engineered products of natural or synthetic biomaterials, often coupled with the strategic placement of cells [1], into three-dimensional networks or bioscaffolds. These bioscaffolds should mimic the physiological and geometric architecture of the body's extracellular matrix (ECM), and hence have a potential to replace damaged or defective tissue, or even entire organs [2,3]. In the event of major injury, the physiological response of the body is one of inflammation, followed by cell mediated wound contraction and the generation of scar tissue in repair [4]; however if a suitable ECM analogue can be implemented, the wound contraction is blocked and instead regeneration is induced [4].…”

Section: Introductionmentioning

confidence: 99%

“…There are considerable challenges associated with this; the developed tissue replacement must be durable [5], nonimmunogenic (or autologous) in nature [6,7], and exhibit the same function and morphology as the target tissue [3]. Successful bioscaffolds must also demonstrate desirable structural strength, dimensions, porosity and pore size [8,9], as well as pore interconnectivity and suitable bioactivity which reflect that of the body's natural ECM to guide cell development and proliferation [10].…”

Section: Introductionmentioning

confidence: 99%

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Coaxial additive manufacture of biomaterial composite scaffolds for tissue engineering

et al. 2014

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An inherent difficulty associated with the application of suitable bioscaffolds for tissue engineering is the incorporation of adequate mechanical characteristics into the materials which recapitulate that of the native tissue, whilst maintaining cell proliferation and nutrient transfer qualities. Biomaterial composites fabricated using rapid prototyping techniques can potentially improve the functionality and patient-specific processing of tissue engineering scaffolds. In this work, a technique for the coaxial melt extrusion printing of core-shell scaffold structures was designed, implemented and assessed with respect to the repeatability, cell efficacy and scaffold porosity obtainable. Encapsulated alginate hydrogel/thermoplastic polycaprolactone (Alg-PCL) cofibre scaffolds were fabricated. Selective laser melting was used to produce a high resolution stainless steel 316 L coaxial extrusion nozzle, exhibiting diameters of 300 μm/900 μm for the inner and outer nozzles respectively. We present coaxial melt extrusion printed scaffolds of Alg-PCL cofibres with ~0.4 volume fraction alginate, with total fibre diameter as low as 600 μm and core material offset as low as 10% of the total diameter. Furthermore the tuneability of scaffold porosity, pore size and interconnectivity, as well as the preliminary inclusion, compatibility and survival of an L-929 mouse fibroblast cell-line within the scaffolds were explored. This preliminary cell work highlighted the need for optimal material selection and further design reiteration in future research.

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