The Genus Helicobacter

Solnick, Jay V.; Vandamme, Peter; Lee, A

doi:10.1007/0-387-30747-8_5

Cited by 14 publications

(15 citation statements)

References 228 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…C. jejuni is responsible for gastroenteritis, the GBS (Guillain-Barré syndrome) [7] and, recently, it has also been found associated with the IPSID (immunoproliferative small intestinal disease) [8], a MALT lymphoma. These two bacteria of known genomic sequences [9,10] are microaerophilic, Gram-negative, flagellate species that are members of the ε-sub-class of the proteobacteria [11,12]. Although chemotherapies for the eradication of these species are currently available, the development of more efficient, inexpensive drugs is needed in order to cope with the drawbacks of the therapies, especially for the treatment of H. pylori [13], and with the continuous emergence of antibiotic resistances.…”

Section: Introductionmentioning

confidence: 99%

Heterologous production in Wolinella succinogenes and characterization of the quinol:fumarate reductase enzymes from Helicobacter pylori and Campylobacter jejuni

Mileni

MacMillan

Tziatzios

et al. 2006

Biochemical Journal

View full text Add to dashboard Cite

The epsilon-proteobacteria Helicobacter pylori and Campylobacter jejuni are both human pathogens. They colonize mucosal surfaces causing severe diseases. The membrane protein complex QFR (quinol:fumarate reductase) from H. pylori has previously been established as a potential drug target, and the same is likely for the QFR from C. jejuni. In the present paper, we describe the cloning of the QFR operons from the two pathogenic bacteria H. pylori and C. jejuni and their expression in Wolinella succinogenes, a non-pathogenic -proteobacterium. To our knowledge, this is the first documentation of heterologous membrane protein production in W. succinogenes. We demonstrate that the replacement of the homologous enzyme from W. succinogenes with the heterologous enzymes yields mutants where fumarate respiration is fully functional. We have isolated and characterized the heterologous QFR enzymes. The high quality of the enzyme preparation enabled us to determine unequivocally by analytical ultracentrifugation the homodimeric state of the three detergent-solubilized heterotrimeric QFR enzymes, to accurately determine the different oxidation-reduction ('redox') midpoint potentials of the six prosthetic groups, the Michaelis constants for the quinol substrate, maximal enzymatic activities and the characterization of three different anti-helminths previously suggested to be inhibitors of the QFR enzymes from H. pylori and C. jejuni. This characterization allows, for the first time, a detailed comparison of the QFR enzymes from C. jejuni and H. pylori with that of W. succinogenes.

show abstract

Section: Introductionmentioning

confidence: 99%

Heterologous production in Wolinella succinogenes and characterization of the quinol:fumarate reductase enzymes from Helicobacter pylori and Campylobacter jejuni

Mileni

MacMillan

Tziatzios

et al. 2006

Biochemical Journal

View full text Add to dashboard Cite

show abstract

“…Though the majority of human gastric infections result from infection with H pylori, a small percentage (0.5-6%) of infections have been attributed to an organism commonly referred to as 'Helicobacter heilmannii ' [3]. This organism is the dominant morphological type of bacterium seen in primates, pigs, and carnivorous mammals [4]. While in humans the resulting gastritis is generally milder than that seen with H pylori, a large study of 202 individuals infected with 'H heilmannii ' showed that this bacterium could also be seen in association with more serious forms of gastric disease including gastric carcinoma [5].…”

Section: Introductionmentioning

confidence: 99%

Gastric B‐cell mucosa‐associated lymphoid tissue (MALT) lymphoma in an animal model of ‘Helicobacter heilmannii’ infection

O'Rourke

Dixon

Jack

et al. 2004

The Journal of Pathology

View full text Add to dashboard Cite

While Helicobacter pylori is accepted as the dominant human gastric bacterial pathogen, a small percentage of human infections have been associated with another organism, commonly referred to as 'Helicobacter heilmannii', which is more prevalent in a range of animal species. This latter bacterium has been seen in association with the full spectrum of human gastric diseases including gastritis, peptic ulceration, and gastric carcinomas, including gastric B-cell mucosa-associated lymphoid tissue (MALT) lymphoma. This study describes an analysis of the pathogenic potential of a number of 'H heilmannii' isolates in an animal model of gastric MALT lymphoma. BALB/c mice were infected with ten different 'H heilmannii' isolates originating from both human and animal hosts. The animals were examined at various time points for up to 28 months after infection. The infected animals initially developed a chronic inflammatory response within 6 months. This histological response increased in severity with the length of infection, with the development of overt lymphoma in some animals 18 months after infection. MALT lymphomas were detected in up to 25% of the infected animals. The prevalence of lymphoma was dependent on the length of infection and the origin of the infecting isolates. A range of other histological features accompanied the lymphocytic infiltration, including invaginations of the gastric epithelium and associated hyperplastic tissue, mucus metaplasia, and a small number of diffuse large B-cell lymphomas. The ability to manipulate experientially the presence of the bacterium in the animal model will allow further studies examining the role of antigen drive in the development of Helicobacter-associated MALT lymphoma.

show abstract

“…Several studies have shown high prevalence of antibodies against H. pylori in abattoir workers, such as veterinarians, butchers and slaughterers, suggesting that H. pylori might be transmitted from animals to man. Dogs and sheep have also been implicated in the transmission of Helicobacter infection [2].…”

Section: Introductionmentioning

confidence: 99%

Epidemiological Study on H. pylori in Cattle and Its Milk with Special Reference to its Zoonotic Importance

El-Gohary¹,

Yousef²,

Mohamed³

2015

Biol Med (Aligarh)

View full text Add to dashboard Cite

This study was carried out to investigate some epidemiological aspects on the occurrence of Helicobacter pylori in cattle, milk and humans at Dakahlia province, Egypt during the period from February 2014 to June 2015. A total of 304 samples including 117 rectal swabs (53 cows and 64 buffaloes) and 85 milk samples (36 cows and 49 buffaloes) sand 102 humans stools were collected and subjected to bacteriological examination by culturing on Columbia Blood Agar (CBA) and biochemically identified. The results showed that the overall occurrence of H. pylori were 21.7% in collected samples. The occurrence in cattle faeces was 18.8% (11.9% in cows and 6.9% in buffaloes). However, the isolation rate from cattle milk was 28.2% (10.5% in cows and 17.7% in buffaloes). Moreover, the frequency distribution of H. pylori from human stool was 19.6%. Concerning animal breed, native breed of cattle and their milk showed higher occurrence (5.2% in cow's faeces, 11.9% in buffalo's faeces, 7% of cow's milk and 17.7% of buffalo's milk). Regarding animal age, the occurrence of H. pylori was increased with increasing age. On the other hand, the frequency distribution of H. pylori was more prevalent in the samples (faeces and milk) collected from Mansoura center. In relation to human samples, with respect to gender, males showed higher isolation rate (11.7%) than females (7.8%).Whereas, frequency of H. pylori in adults (4.9%) was higher than young (1.96%).On the other hand, H. pylori was more frequently isolated from patients (8.8%) with gastrointestinal disorders. Moreover, the occurrence of H. pylori was higher in human samples collected from persons of occupations related to animals such as veterinarians (6.8%), dairy workers, and farmers (3.92% each) than others. It could be concluded that H. pylori could be isolated from cattle, milk and humans with recognizable percentages, suggesting its zoonotic significance and role played by cattle especially buffaloes and its milk as potential reservoir and source of human infection. The zoonotic significance for H. pylori as well as the recommended preventive measures which should be taken to avoid the risk of contamination of milk and human infection were fully discussed. B io lo g y and M e d ic in e

show abstract

The Genus Helicobacter

Cited by 14 publications

References 228 publications

Heterologous production in Wolinella succinogenes and characterization of the quinol:fumarate reductase enzymes from Helicobacter pylori and Campylobacter jejuni

Heterologous production in Wolinella succinogenes and characterization of the quinol:fumarate reductase enzymes from Helicobacter pylori and Campylobacter jejuni

Gastric B‐cell mucosa‐associated lymphoid tissue (MALT) lymphoma in an animal model of ‘Helicobacter heilmannii’ infection

Epidemiological Study on H. pylori in Cattle and Its Milk with Special Reference to its Zoonotic Importance

Contact Info

Product

Resources

About