Background: Acclimatization is the last stage of tissue culture that aims to adapt plantlets to environmental conditions. Nutrient availability needs to be considered in this critical phase. This research was conducted to determine the right type of organic manure and AMF dose to support the post-acclimatization growth of banana cv. Barangan. Methods: The study using a completely randomized design (CRD) based on factorial with two treatment factors. The first factor is the type of organic manures: goat manure, cow manure, vermicompost and compost. The second factor is AMF doses: 0, 5, 10, 15 g/plant. Observed variables include the plant height, the pseudo-stem diameter, leaf number, leaf length and width and the percentage of mycorrhiza infections at the plant roots. Result: The results showed an interaction between the type of organic manure and AMF dosage at the leaf length and width, but not on the plant height, the pseudo-stem diameter and leaf number. The application of various organic manures types has a significant effect on the plant height, the pseudo-stem diameter, leaf length and width, but no significant effect on the leaf number. The application of various doses of AMF has no significant impact on all of the observed variables.