The heat shock response is dependent on the external environment and on rapid ionic balancing by pharmacological agents in Saccharomyces cerevisiae

Abstract

I . V O V O U , A . D E L I T H E O S A N D E . T I L I G A D A . 2004.Aims: To investigate whether non-preconditioned yeast cells survive under heat shock, when placed in growth medium originated from protected cells and to provide insights into the ionic contribution in the response. Methods and Results: The heat shock response was investigated by determining cell viability following exposure of yeast cells to 53°C for 30 min, either in the absence or presence of drugs. Preconditioning was performed by incubating the cultures at 37°C for 2 h. Under heat shock, non-preconditioned cell survival was significantly enhanced by the presence of the cell-free supernatant of resistant cultures. Addition of omeprazole or tetraethylammonium ions during the heat shock resulted in similar increases. Neither amiodarone nor mepivacaine showed any analogous effect. Omeprazole enhanced survival when added before the heat shock, while amiodarone exhibited a cytocidic action. Conclusions: Rapid balancing of ions may contribute to cell survival during heat shock, while survival under mild stress could probably be co-ordinated by additional events. Significance and Impact of the Study: Evidence is provided for the implication of the external environment and ionic homeostasis in the survival of yeast cells under unfavourable environmental conditions. This knowledge may be of importance in controlling both fermentation and therapeutic approaches.

Protective effect of salicylates against hydrogen peroxide stress in yeast

“…The reduction in cell proliferation potential and the formation of size‐variable CFUs following stress with 1·5 mmol l −1 H 2 Ο 2 for 1 h were comparable to the phenotype observed during the well‐established heat shock response induced by thermal preconditioning (Vovou et al. 2004; Papamichael et al.…”

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“…The propagation of signals determines the functional integration of deleterious stimuli and cell survival or death [13]. Moreover, the critical role of the microenvironment in providing cells with survival signals should be taken into consideration [14]. cellular target, while the remarkable plasticity of cancer cells and the course of the disease may alter the pharmacological effect.…”

A subset of histamine receptor ligands improve thermotolerance of the yeast Saccharomyces cerevisiae

“…Beyond imbalance of protein homoeostasis, stress may induce potentially detrimental effects to cells, including damages in the cytoskeleton, disruption of transport processes (Vovou et al . ), alterations in phosphorylation (Tiligada et al . ), intracellular translocation (Tiligada ) and organelle delocalization (Morano et al .…”

“…Among them, heat shock proteins (Hsps) have received great attention to date since many function as molecular chaperones regulating protein structure and function and play critical roles in almost every cellular process under both stress and nonstress conditions (Morimoto 2008;Morano et al 2012;Richter et al 2012). Beyond imbalance of protein homoeostasis, stress may induce potentially detrimental effects to cells, including damages in the cytoskeleton, disruption of transport processes (Vovou et al 2004), alterations in phosphorylation (Tiligada et al 1999), intracellular translocation (Tiligada 2006a) and organelle delocalization (Morano et al 2012).…”